TRPV5 and TRPV6 are expressed in placenta and bone tissues during pregnancy in mice

Li, S. H; Yin, Huiyong; Ren, Mingli; Wu, Ming; Huang, Xiao; Li, J. J; Luan, Yunpeng; Wu, Yueyue

doi:10.1080/10520295.2018.1548710

Cited by 12 publications

(6 citation statements)

References 22 publications

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“…The TRPV6 gene encodes a member of a family of multipass membrane proteins that function as calcium channels. TRPV6 is expressed in the human placenta, primarily in trophoblasts 21 , and its expression in the placenta and fetal bone tissue persists throughout mouse pregnancy 22 . The CALCB gene encodes calcitonin-related beta-peptide (CGRP), which is a neurotransmitter.…”

Section: Discussionmentioning

confidence: 99%

Identification of differentially methylated genes in first-trimester placentas with trisomy 16

Tolmacheva

Vasilyev

Nikitina

et al. 2022

Sci Rep

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The presence of an extra chromosome in the embryo karyotype often dramatically affects the fate of pregnancy. Trisomy 16 is the most common aneuploidy in first-trimester miscarriages. The present study identified changes in DNA methylation in chorionic villi of miscarriages with trisomy 16. Ninety-seven differentially methylated sites in 91 genes were identified (false discovery rate (FDR) < 0.05 and Δβ > 0.15) using DNA methylation arrays. Most of the differentially methylated genes encoded secreted proteins, signaling peptides, and receptors with disulfide bonds. Subsequent analysis using targeted bisulfite massive parallel sequencing showed hypermethylation of the promoters of specific genes in miscarriages with trisomy 16 but not miscarriages with other aneuploidies. Some of the genes were responsible for the development of the placenta and embryo (GATA3-AS1, TRPV6, SCL13A4, and CALCB) and the formation of the mitotic spindle (ANKRD53). Hypermethylation of GATA3-AS1 was associated with reduced expression of GATA3 protein in chorionic villi of miscarriages with trisomy 16. Aberrant hypermethylation of genes may lead to a decrease in expression, impaired trophoblast differentiation and invasion, mitotic disorders, chromosomal mosaicism and karyotype self-correction via trisomy rescue mechanisms.

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Section: Discussionmentioning

confidence: 99%

Identification of differentially methylated genes in first-trimester placentas with trisomy 16

Tolmacheva

Vasilyev

Nikitina

et al. 2022

Sci Rep

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“…TRPV6 was suggested to be a marker for implantation in the porcine endometrium [36]. In murine embryos TRPV6 is responsible for calcium ion homeostasis [37], whereas silencing its expression in human trophoblast cells resulted in diminished cell proliferation and induced apoptosis [38]. Interestingly, among the top five up-regulated genes in E2-treated and day 12 of pregnant endometrium were other genes related to calcium binding S100A8, S100A9, and S100A12.…”

Section: Estradiol-17β Affects the Expression Of Genes Involved In Sementioning

confidence: 99%

Estradiol-17β-Induced Changes in the Porcine Endometrial Transcriptome In Vivo

Kaczyński

Bauersachs

Baryla

et al. 2020

IJMS

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Estradiol-17β (E2) is a key hormone regulating reproductive functions in females. In pigs, E2, as the main conceptus signal, initiates processes resulting in prolonged corpus luteum function, embryo development, and implantation. During early pregnancy the endometrium undergoes morphological and physiological transitions that are tightly related to transcriptome changes. Recently, however, the importance of E2 as a primary conceptus signal in the pig has been questionable. Thus, the aim of the present study was to determine the effects of E2 on the porcine endometrial transcriptome in vivo and to compare these effects with transcriptome profiles on day 12 of pregnancy. Microarray analysis revealed differentially expressed genes (DEGs) in response to E2 with overrepresented functional terms related to secretive functions, extracellular vesicles, cell adhesion, proliferation and differentiation, tissue rearrangements, immune response, lipid metabolism, and many others. Numerous common DEGs and processes for the endometrium on day 12 of pregnancy and E2-treated endometrium were identified. In summary, the present study is the first evidence for the effect of E2 on transcriptome profiles in porcine endometrium in vivo in the period corresponding to the maternal recognition of pregnancy. The presented results provide a valuable resource for further targeted studies considering genes and pathways regulated by conceptus-derived estrogens and their role in pregnancy establishment.During early pregnancy in the pig, porcine conceptuses (embryos with associated membranes) secrete E2, which is regarded as the pregnancy recognition signal required for prolonged progesterone synthesis and secretion by corpora lutea (CLs). Porcine conceptuses secrete estrogens in a biphasic manner-the first increase of conceptus-derived estrogens occurs on days 11-13 after fertilization and the second is between days 15 and 25-30 after fertilization (reviewed in [2]). However, the levels of conceptus-derived estrogens in the uterine lumen may also vary depending on the number of conceptuses [1]. The period of elevated E2 synthesis and secretion by porcine conceptuses between days 11-13 of pregnancy is a process defined as the maternal recognition of pregnancy [5]. This is the critical period for establishment and development of pregnancy as the highest mortality rate of embryos in animals including pigs is observed during the peri-implantation period [6]. Embryonic estrogen synthesis and secretion not only prolongs the CL lifespan but also enhances P4-induced endometrial receptivity for implantation. Following the decrease of the progesterone receptor expression in the endometrial luminal and glandular epithelia, the expression of the estrogen receptor (ESR1) is up-regulated in these structures, which in turn is important for the cell-specific responses to conceptus estrogens released on day 12 of pregnancy [7]. Estrogen is involved in stimulation of uterine secretory activity [8], increased blood flow [9], endometrial edema [10], and the regul...

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“…More recently, TRPV5 and TRPV6 were assessed in the placenta by another group, describing expression of TRPV6 in the labyrinth and the junctional zone. In addition, they reported the strongest expression of TRPV6 in the junctional zone, while the decidua lacked TRPV6 [82]. Adding to this discord, Fecher-Trost et al, reported TRPV6 protein expression in the labyrinth and the decidua, but not in the junctional zone [83].…”

Section: A Role For Trp Channels In Placental Transport Of Cationsmentioning

confidence: 99%

Mapping the expression of transient receptor potential channels across murine placental development

Clercq

Pérez-García

Bree

et al. 2021

Cell. Mol. Life Sci.

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Transient receptor potential (TRP) channels play prominent roles in ion homeostasis by their ability to control cation influx. Mouse placentation is governed by the processes of trophoblast proliferation, invasion, differentiation, and fusion, all of which require calcium signaling. Although certain TRP channels have been shown to contribute to maternal–fetal transport of magnesium and calcium, a role for TRP channels in specific trophoblast functions has been disregarded. Using qRT-PCR and in situ hybridisation, the spatio-temporal expression pattern of TRP channels in the mouse placenta across gestation (E10.5–E18.5) was assessed. Prominent expression was observed for Trpv2, Trpm6, and Trpm7. Calcium microfluorimetry in primary trophoblast cells isolated at E14.5 of gestation further revealed the functional activity of TRPV2 and TRPM7. Finally, comparing TRP channels expression in mouse trophoblast stem cells (mTSCs) and mouse embryonic stem cells (mESC) confirmed the specific expression of TRPV2 during placental development. Moreover, TRP channel expression was similar in mTSCs compared to primary trophoblasts and validate mTSC as a model to study TRP channels in placental development. Collectivity, our results identify a specific spatio-temporal TRP channel expression pattern in trophoblasts, suggesting a possible involvement in regulating the process of placentation.

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TRPV5 and TRPV6 are expressed in placenta and bone tissues during pregnancy in mice

Cited by 12 publications

References 22 publications

Identification of differentially methylated genes in first-trimester placentas with trisomy 16

Identification of differentially methylated genes in first-trimester placentas with trisomy 16

Estradiol-17β-Induced Changes in the Porcine Endometrial Transcriptome In Vivo

Mapping the expression of transient receptor potential channels across murine placental development

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