TRPV5 in renal tubular calcium handling and its potential relevance for nephrolithiasis

Wijst, Jenny van der; Goor, M.K. van; Schreuder, Michiel F.; Hoenderop, Joost G. J.

doi:10.1016/j.kint.2019.05.029

Cited by 20 publications

(11 citation statements)

References 97 publications

(153 reference statements)

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“…In an infection-induced urolithiasis rat model, the activities of calcium related ion channels were reported to be influenced by bacterial infection, and correlated with chronic inflammation of the kidney along with rapid aggregation of stones [29]. Moreover, transient receptor potential vanilloid 5 (TRPV5), a member of the transient receptor potential family of ion channels, has also been proved to be closely associated with urinary stone formation [30]. In the present study, our results revealed enriched ion channels pathway in the urine of nephrolithiasis patients, but its specific role in kidney stone formation still remains unclear and needs further investigation.…”

Section: Discussionmentioning

confidence: 99%

Profiling the urinary microbiome in men with calcium-based kidney stones

Xie

Huang

et al. 2020

BMC Microbiol

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Background: The dogma that urine is sterile in healthy individuals has been overturned by recent studies applying molecular-based methods. Mounting evidences indicate that dysbiosis of the urinary microbiota is associated with several urological diseases. In this study, we aimed to investigate the urinary microbiome of male patients with calcium-based kidney stones and compare it with those of healthy individuals. Results: The diversity of the urinary microbiota in kidney stone patients was significantly lower than that of healthy controls based on the Shannon and Simpson index (P < 0.001 for both indices). The urinary microbiota structure also significantly differed between kidney stone patients and healthy controls (ANOSIM, R = 0.11, P < 0.001). Differential representation of inflammation associated bacteria (e.g., Acinetobacter) and several enriched functional pathways were identified in the urine of kidney stones patients. Meanwhile, we found the species diversity, overall composition of microbiota and predicted functional pathways were similar between bladder urine and renal pelvis urine in kidney stone patients. Conclusions:A marked dysbiosis of urinary microbiota in male patients with calcium-based kidney stones was observed, which may be helpful to interpret the association between bacteria and calcium-based kidney stones.

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Section: Discussionmentioning

confidence: 99%

Profiling the urinary microbiome in men with calcium-based kidney stones

Xie

Huang

et al. 2020

BMC Microbiol

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“…The TRPV subfamily has been involved in the pathophysiology of several gastrointestinal diseases such as gastroesophageal reflux disease (GERD), nonesophageal reflux disease (NERD), and irritable bowel syndrome (IBS) [17,18]. Thus, in this study, we determined the gene and protein expression of the other members of the TRPV subfamily, in order to provide the characterization of these receptors in patients with active and remission UC.…”

Section: Discussionmentioning

confidence: 99%

“…They are responsible for limiting the rate of Ca 2+ entry into cells during transcellular Ca 2+ reabsorption [12][13][14]. The functional roles of TRPV5 and TRPV6 are interconnected with each other, given that TRPV5 knockout mice upregulate their intestinal TRPV6 expression to compensate for the negative Ca 2+ balance caused by the loss of TRPV5mediated Ca 2+ reabsorption in the kidney [15][16][17]. Changes in the expression of TRPV5 and TRPV6 could be involved in the development of IBD and bone-related extraintestinal manifestations [18].…”

Section: Introductionmentioning

confidence: 99%

TRPV Subfamily (TRPV2, TRPV3, TRPV4, TRPV5, and TRPV6) Gene and Protein Expression in Patients with Ulcerative Colitis

Mauriño

Fonseca‐Camarillo

Furuzawa‐Carballeda

et al. 2020

Journal of Immunology Research

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Introduction. TRPVs are a group of receptors with a channel activity predominantly permeable to Ca2+. This subfamily is involved in the development of gastrointestinal diseases such as ulcerative colitis (UC). The aim of the study was to characterize the gene and protein expression of the TRPV subfamily in UC patients and controls. Methods. We determined by quantitative PCR the gene expression of TRPV2, TRPV3, TRPV4, TRPV5, and TRPV6 in 45 UC patients (29 active UC and 16 remission UC) and 26 noninflamed controls. Protein expression was evaluated in 5 μm thick sections of formalin-fixed, paraffin-embedded tissue from 5 customized severe active UC patients and 5 control surgical specimens. Results. TRPV2 gene expression was increased in the control group compared with active UC and remission patients (P=0.002 and P=0.05, respectively). TRPV3 gene expression was significantly higher in controls than in active UC patients (P=0.002). The gene expression of TRPV4 was significantly higher in colonic tissue from patients with remission UC compared with active UC patients (P=0.05) and controls (P=0.005). TRPV5 had significantly higher mRNA levels in a control group compared with active UC patients (P=0.02). The gene expression of TRPV6 was significantly higher in the colonic tissue from patients with active UC compared with the control group (P=0.05). The protein expression of TRPV2 was upregulated in the mucosa and submucosa from the controls compared with the UC patients (P≤0.003). The protein expression of TRPV3 and TRPV4 was upregulated in all intestinal layers from the controls compared with the UC patients (P<0.001). TRPV5 was upregulated in the submucosa and serosa from the controls vs. UC patients (P<0.001). TRPV6 was upregulated in all intestinal layers from the UC patients vs. controls (P≤0.001). Conclusion. The TRPV subfamily clearly showed a differential expression in the UC patients compared with the controls, suggesting their role in the pathophysiology of UC.

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“…TRPV5 also undergoes Ca 2+ -induced inactivation, which is mediated by calmodulin (CaM) binding to the channel ( de Groot et al, 2011 ). The precise balance between PI(4,5)P 2 activation and CaM inhibition determines the basal activity of the channel ( Cao et al, 2013 ; van der Wijst et al, 2019 ). Recently, TRPV5 structural studies in the presence of CaM and PI(4,5)P 2 revealed how this channel’s constitutive activity is accomplished.…”

Section: Introductionmentioning

confidence: 99%