True syntopy between chromosomal races of the Cryptocercus punctulatus wood-roach species complex

Garrick, Ryan C.

doi:10.1007/s00040-016-0475-1

Cited by 6 publications

(6 citation statements)

References 11 publications

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“…Divergent mtDNA lineages have been documented within numerous species of forest invertebrates from the southern Appalachian Mountains and surrounding areas, but in most cases, spatial distributions are allopatric. For example, the wood-feeding cockroach, Cryptocercus punctulatus (Blattodea: Cryptocercidae), is comprised of five mtDNA clades [43,44], and, although three of these occur in close proximity in the Great Smoky Mountains, they are locally non-overlapping (but see Garrick [45] for an anomaly). Similarly, the leaf-litter-dwelling flightless weevil, Eurhoptus pyriformis (Coleoptera: Curculionidae), is comprised of at least two lineages that show a spatial-genetic discontinuity in the vicinity of the Smoky Mountains [46].…”

Section: Resultsmentioning

confidence: 99%

Two Divergent Genetic Lineages within the Horned Passalus Beetle, Odontotaenius disjunctus (Coleoptera: Passalidae): An Emerging Model for Insect Behavior, Physiology, and Microbiome Research

Garrick

Dickinson

Reppel

et al. 2019

Insects

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The horned passalus (Odontotaenius disjunctus) is one of the most extensively studied saproxylic beetles in the eastern United States. For several decades this species has been the subject of investigations into the behaviors associated with subsociality as well as physiological responses to stress, and, most recently, the composition of its gut microbiome has been closely examined. However, no published study to date has characterized this beetle’s broad-scale population genetic structure. Here, we conducted intensive geographic sampling throughout the southern Appalachian Mountains and surrounding areas and then assessed mitochondrial DNA (mtDNA) sequence variation among individuals. Unexpectedly, we discovered two divergent, yet broadly sympatric, mtDNA clades. Indeed, the magnitude of divergence between- vs. within-clades ranged from 5.9 to 7.5×, depending on the dataset under consideration, and members of the two lineages were often syntopic (i.e., found in the same rotting log). Given the potential implications for past and future studies on behavior, physiology, and the gut microbiome, we developed a simple cost-efficient molecular assay (i.e., polymerase chain reaction restriction fragment length polymorphism; PCR-RFLP) to rapidly determine mtDNA clade membership of O. disjunctus individuals. We suggest that the evolutionary processes that gave rise to the emergence and persistence of divergent sympatric lineages reported here warrant investigation, as this type of spatial-genetic pattern appears to be rare among southern Appalachian forest invertebrates.

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Section: Resultsmentioning

confidence: 99%

Two Divergent Genetic Lineages within the Horned Passalus Beetle, Odontotaenius disjunctus (Coleoptera: Passalidae): An Emerging Model for Insect Behavior, Physiology, and Microbiome Research

Garrick

Dickinson

Reppel

et al. 2019

Insects

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“…2). Indeed, C. punctulatus from highly divergent genetic lineages—including those that likely differ in chromosome number—may occasionally co-inhabit the same log (Garrick, 2016). Accordingly, depending on the goals of the study, it may be prudent to sample multiple individuals from a log.…”

Section: Discussionmentioning

confidence: 99%

“…Two or three adults per log (mean = 2.78) were used for molecular analyses. DNA extraction and polymerase chain reaction amplification, sequencing, alignment and validation of data from mtDNA cytochrome oxidase subunit I (COI) and subunit II (COII) genes followed Garrick (2016), and characteristics of the molecular dataset are summarized in Table 1. For each individual, COI+COII sequences were concatenated (1,125-bp), and each rotting log was classified as containing cockroaches with the same mtDNA haplotype vs .…”

Section: Methodsmentioning

confidence: 99%

Genetic insights into family group co-occurrence inCryptocercus punctulatus, a sub-social woodroach from the southern Appalachian Mountains

Garrick

2017

PeerJ

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The wood-feeding cockroach Cryptocercus punctulatus Scudder (Blattodea: Cryptocercidae) is an important member of the dead wood (saproxylic) community in montane forests of the southeastern United States. However, its population biology remains poorly understood. Here, aspects of family group co-occurrence were characterized to provide basic information that can be extended by studies on the evolution and maintenance of sub-sociality. Broad sampling across the species’ range was coupled with molecular data (mitochondrial DNA (mtDNA) sequences). The primary questions were: (1) what proportion of rotting logs contain two or more different mtDNA haplotypes and how often can this be attributed to multiple families inhabiting the same log, (2) are multi-family logs spatially clustered, and (3) what levels of genetic differentiation among haplotypes exist within a log, and how genetically similar are matrilines of co-occurring family groups? Multi-family logs were identified on the premise that three different mtDNA haplotypes, or two different haplotypes among adult females, is inconsistent with a single family group founded by one male–female pair. Results showed that of the 88 rotting logs from which multiple adult C. punctulatus were sampled, 41 logs (47%) contained two or more mtDNA haplotypes, and at least 19 of these logs (22% overall) were inferred to be inhabited by multiple families. There was no strong evidence for spatial clustering of the latter class of logs. The frequency distribution of nucleotide differences between co-occurring haplotypes was strongly right-skewed, such that most haplotypes were only one or two mutations apart, but more substantial divergences (up to 18 mutations, or 1.6% uncorrected sequence divergence) do occasionally occur within logs. This work represents the first explicit investigation of family group co-occurrence in C. punctulatus, providing a valuable baseline for follow-up studies.

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“…were used for molecular analyses. DNA extraction and polymerase chain reaction amplification, sequencing, alignment and validation of data from mtDNA cytochrome oxidase subunit I (COI) and subunit II (COII) genes followed Garrick (2016), and characteristics of the molecular dataset are summarized in Table 1. For each individual, COI+COII sequences were concatenated (1125bp), and each rotting log was classified as containing cockroaches with the same mtDNA haplotype vs. two or more different mtDNA haplotypes (i.e., single-haplotype vs. multihaplotype logs).…”

Section: Sampling and Rotting Log Classificationmentioning

confidence: 99%

“…Even though the majority of co-occurring haplotypes have few mutational differences, some divergences are more substantial (Figure 2). Indeed, C. punctulatus from highly divergent genetic lineages-including those that likely differ in chromosome number-may occasionally co-inhabit the same log (Garrick 2016). Accordingly, depending on the goals of the study, it may be prudent to sample multiple individuals from a log.…”

Section: Genetic Differentiationmentioning

confidence: 99%

Peer Review #2 of "Genetic insights into family group co-occurrence in Cryptocercus punctulatus, a sub-social woodroach from the southern Appalachian Mountains (v0.1)"

2017

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The wood-feeding cockroach Cryptocercus punctulatus Scudder (Blattodea: Cryptocercidae) is an important member of the dead wood (saproxylic) community in montane forests of the southeastern United States. However, its population biology remains poorly understood. Here, aspects of family group co-occurrence were characterized in order to provide basic information that can be extended by studies into the evolution and maintenance of sub-sociality. Broad sampling across the species' range was coupled with molecular data [mitochondrial DNA (mtDNA) sequences]. The primary questions were: (1) what proportion of rotting logs contain two or more different mtDNA haplotypes and how often can this be attributed to multiple families inhabiting the same log, (2) are multi-family logs spatially clustered, and (3) what levels of genetic differentiation among haplotypes exist within a log, and how genetically similar are matrilines of co-occurring family groups? Multi-family logs were identified on the premise that three different mtDNA haplotypes, or two different haplotypes among adult females, is inconsistent with a single family group founded by one male-female pair. Results showed that of the 88 rotting logs from which multiple adult C. punctulatus were sampled, 41 logs (47%) contained two or more mtDNA haplotypes, and at least19 of these logs (22% overall) were inferred to be inhabited by multiple families. There was no strong evidence for spatial clustering of the latter class of logs. The frequency distribution of nucleotide differences between co-occurring haplotypes was strongly right-skewed, such that most haplotypes were only one or two mutations apart, but more substantial divergences (up to 18 mutations, or 1.6% uncorrected sequence divergence) do occasionally occur within logs. This work represents the first explicit investigation of family group co-occurrence in C. punctulatus, providing a valuable baseline for follow-up studies.

show abstract

True syntopy between chromosomal races of the Cryptocercus punctulatus wood-roach species complex

Cited by 6 publications

References 11 publications

Two Divergent Genetic Lineages within the Horned Passalus Beetle, Odontotaenius disjunctus (Coleoptera: Passalidae): An Emerging Model for Insect Behavior, Physiology, and Microbiome Research

Two Divergent Genetic Lineages within the Horned Passalus Beetle, Odontotaenius disjunctus (Coleoptera: Passalidae): An Emerging Model for Insect Behavior, Physiology, and Microbiome Research

Genetic insights into family group co-occurrence inCryptocercus punctulatus, a sub-social woodroach from the southern Appalachian Mountains

Peer Review #2 of "Genetic insights into family group co-occurrence in Cryptocercus punctulatus, a sub-social woodroach from the southern Appalachian Mountains (v0.1)"

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