True-to-Typeness Status of Certified Parent and Commercial Citrus Plants

Abstract

In 1987 Indonesia started to produce virus-free (VF) mother plants by using shoot-tip-grafting (STG) technique. To date, the propagation and distribution of VF plants are made through several stages of certified protected parent plants, i.e. Foundation Blocks (FB) and Budwood Multiplication Blocks (BMB). FB, BMB, and commercial farmer plants are derived from propagated of STG, FB, and BMB trees, respectively. The research was done in June -December 2017 at Plant Breeding Laboratory, Indonesian Citrus and Subtropical Fruit Research Institut (ICSFRI). Eighty-three and 66 samples were derived from parent and seedling and production, respectively. The cultivars used were Mandarin Batu 55, SoE, Terigas, and Tangerine Pontianak, and Banjar collected from five provinces. As a control for every cultivar, a single collection mother tree was used. The method used was based on the similarities between DNA band plant samples and control produced both by five ISSR and retrotransposon markers. The purpose of this research was to evaluate genetic deviations of parent virus-free and commercial citrus plants derived from five provinces. The results indicated that 5.1 and 8.2 % were off type plants, with the degree of genetic deviation was among 4.9-36.4% and 3.7-23.1% for parent and commercial samples, respectively. For parent plants of cv. Batu 55 and Pontianak were all on type, but not for others samples. This result indicated that management both at screen house and field needs to be done carefully, so that the quality of the commercial seeds and plants produced remain the same as the parent.KEY WORDS Budwood multiplication block, cultivar, citrus, DNA, foundation block, true to type, virus.

Genetic Variability Studies of Indonesian Garlic (Allium Sativum L.) Accessions Based on Morphological Traits

“…Hence, the dependency of Indonesia on imported garlic has been increasing for decades (Purba et al, 2022). Despite the urgency for developing superior varieties, very limited exploration and characterization of Indonesia's local garlic accessions transpired (Hardiyanto et al, 2007;Wahyuni et al, 2023). Moreover, most previous studies discussed their agronomical aspects, such as, adaptation (Kurniaty et al, 2022;Saidah et al, 2022), cultivation techniques (Aswani et al, 2022;Dianawati et al, 2022), temperature, and photoperiod effect on their vegetative growth (Azmi et al, 2022;Kendarini et al, 2022).…”