2024
DOI: 10.1101/2024.12.11.627906
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Truncated and “double-headed” derivatives of TO1-B dye for Mango-based imaging systems with increased brightness and selectivity and large Stocks shift

Julia I. Svetlova,
Georgy K. Slushko,
Anna S. Fayzieva
et al.

Abstract: Fluorogenic dyes that light up in complexes with genetically encodable RNA aptamers are increasingly used for intracellular RNA imaging. The growing toolbox of dyes and aptamers allows simultaneous or sequential monitoring of different RNAs. However, few orthogonal aptamer-dye pairs meet all the requirements of multiplex imaging in terms of brightness, contrast, etc., thus necessitating their optimization and further diversification. Here, we report new fluorogenic ligands for the Mango II aptamer, one of the … Show more

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