Trypanocidal activity of extracts and fractions of Bertholletia excelsa

Campos, Francinete Ramos; Januário, Ana Helena; Rosas, Lisandra Vieira; Nascimento, Samara K. R.; Pereira, Paulo Sérgio; França, Suzelei de Castro; Cordeiro, M S C; Toldo, Míriam Paula Alonso; Albuquerque, Sérgio de

doi:10.1016/j.fitote.2004.09.008

Cited by 19 publications

(12 citation statements)

References 7 publications

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“…A correlation of restricted knowledge of this could be demonstrated by the experimental demonstration of its high antiplasmodial activity in vitro (IC 50 between 2.5 -7.5 µg/ml). Anti-parasite activity of B. excelsa was also demonstrated previously against Trypanosoma cruzi (Campos et al, 2005).…”

Section: Discussionsupporting

confidence: 76%

Ethnopharmacological evaluation of medicinal plants used against malaria by quilombola communities from Oriximiná, Brazil

Oliveira

Krettli

Aguiar

et al. 2015

Journal of Ethnopharmacology

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A strong correlation between the consensus of the informants from quilombola communities living in a malaria endemic area and the salience index indicating antiplasmodial activity was observed, where the ethnospecies mostly cited to be effective against malaria produced the most active plant extracts in vitro. It was also evident from the data that these groups approached the treatment of malaria with an holistic view, making use of purgative, depurative, emetic and adaptogen plants.

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Section: Discussionsupporting

confidence: 76%

Ethnopharmacological evaluation of medicinal plants used against malaria by quilombola communities from Oriximiná, Brazil

Oliveira

Krettli

Aguiar

et al. 2015

Journal of Ethnopharmacology

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“…MCC is rich in AAA and t-DCTN but also contains other classes of components (sesquiterpenes, terpenes, isoquinolinic alkaloids, phenolic compounds and fatty acids) (Maciel et al 2000(Maciel et al , 2003a(Maciel et al , b, 2007Souza et al 2006). Another possibility for the higher activity seen in MCC is its polarity (Maciel et al 1998), a characteristic that was associated with the trypanocidal activity of other vegetal extracts (Campos et al 2005;Grael et al 2005). Interestingly, it was observed for the replicative forms, i.e.…”

Section: Resultsmentioning

confidence: 99%

Croton cajucara crude extract and isolated terpenes: activity on Trypanosoma cruzi

et al. 2010

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Croton cajucara is a plant found in the Amazon region and is known for its medicinal properties. The effects of the methanolic extract of the stem bark of C. cajucara (MCC) and of the isolated terpenes, trans-dehydrocrotonin (t-DCTN) and acetyl aleuritolic acid (AAA), were investigated using four isolates of Trypanosoma cruzi. In assays with trypomastigotes, the extract was more active than the isolated compounds, presenting IC(50) in the range of 10 to 50 μg/mL. The trypanocidal effect of MCC, AAA and benznidazole was significantly higher in the GLT291 and C45 strains, which were recently isolated from wild animals. MCC and AAA caused a dose-dependent inhibition of epimastigote proliferation. In assays using intracellular amastigotes, AAA and MCC reduced the percent of infection and the endocytic index after 96 h of treatment, at concentrations that were non-toxic to the host cells. MCC inhibited the trypanothione reductase pathway in both epimastigotes and trypomastigotes of all the subpopulations. The absence of AAA activity on the trypanothione reductase pathway in epimastigotes of Dm28c suggests heterogeneity of the biochemical profile between this clone and the three strains. Epimastigotes and trypomastigotes (GLT291) were treated for 24 h with MCC or AAA, and both induced alterations of the plasma membrane, while AAA-treated epimastigotes also displayed mitochondrial damage.

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“…Thus, the use of natural products with recognized low toxicity and potent antioxidative activity may be more appropriate than synthetic antioxidants if their trypanocidal activity against the infective trypomastigote form of T. cruzi proves to be adequate (Campos et al 2005). Therefore, in the present work we have investigated the effect of essential oils and constituents obtained from oregano and thyme on growth in the cell viability and ultrastructure of diverse evolutive forms of T. cruzi.…”

Section: Introductionmentioning

confidence: 99%

Effect of oregano (Origanum vulgare L.) and thyme (Thymus vulgaris L.) essential oils on Trypanosoma cruzi (Protozoa: Kinetoplastida) growth and ultrastructure

et al. 2006

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In the present work, we have investigated the effect of essential oils obtained from Origanum vulgare L. (oregano) and Thymus vulgaris L. (thyme) on growth and ultrastructure of diverse evolutive forms of Trypanosoma cruzi. Culture epimastigotes and bloodstream trypomastigotes were incubated for 24 h with different concentrations of oregano or thyme essential oils and with thymol (the main constituent of thyme), and the inhibitory concentration (IC)(50) was determined by cell counting. Crude extract of oregano essential oil inhibited epimastigote growth (IC(50)/24 h = 175 microg/ml) and also induced trypomastigote lysis (IC(50)/24 h = 115 microg/ml). Thyme essential oil presented IC(50)/24 h values of 77 microg/ml for epimastigotes and 38 mug/ml for trypomastigotes, while treatment with thymol resulted in an IC(50)/24 h of 62 microg/ml for epimastigotes and 53 microg/ml for trypomastigotes. Scanning electron microscopy of treated cells showed few morphological alterations at the plasma membrane. Observation by transmission electron microscopy showed cytoplasmic swelling with occasional morphological alterations in plasma and flagellar membrane. Our data indicate that oregano and thyme essential oils are effective against T. cruzi, with higher activity of thyme, and that thymol may be the main component responsible for the trypanocidal activity.

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Trypanocidal activity of extracts and fractions of Bertholletia excelsa

Cited by 19 publications

References 7 publications

Ethnopharmacological evaluation of medicinal plants used against malaria by quilombola communities from Oriximiná, Brazil

Ethnopharmacological evaluation of medicinal plants used against malaria by quilombola communities from Oriximiná, Brazil

Croton cajucara crude extract and isolated terpenes: activity on Trypanosoma cruzi

Effect of oregano (Origanum vulgare L.) and thyme (Thymus vulgaris L.) essential oils on Trypanosoma cruzi (Protozoa: Kinetoplastida) growth and ultrastructure

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