Trypanocidal Potentials of Azadirachta indica: In Vivo Activity of Leaf Extract against Trypanosoma brucei brucei.

Abstract

SummaryAn aqueous extract of defatted leaves of Azadirachta indica was found to possess trypanocidal activity against Trypanosoma brucei brucei. Column chromatography of the crude extract gave three fractions, I, II, and III, that eluted with ethylacetate/methanol, benzene/ methanol, and acetic acid/methanol, respectively. Of these extracts, only fraction III retained trypanocidal properties and cured mice chronically infected with Trypanosoma brucei brucei. Histopathological studies of the brains, livers, hearts, and spleens of the treated mice showed no cellular infiltrations. These findings are discussed in relation to trypanosome chemotherapy.

In vivo trypanocidal activity of Annona senegalensis Pers. leaf extract against Trypanosoma brucei brucei

“…Inoculation of mice with CSF obtained from the second surviving mouse was done as described by Nok et al (1993). Briefly, the hair on the back of the surviving mouse was shaved and it was positioned such that its head touched the limbs.…”

Toxicity and potential anti-trypanosomal activity of ethanolic extract of Azadirachta indica (Maliacea) stem bark: An in vivo and in vitro approach using Trypanosoma brucei

“…The acclaimed neem tree (Azadirachta indica), for instance, has been found to be effective against tuberculosis and skin infections (Vietmeyer, 2002), pests and nematodes of livestock (Schmutterer, 1989), malaria due to Plasmodia falciparum infection (Khalid et al, 1986), Setaria cervi infection of cattle (Vandna et al, 2005) and coccidiosis of chicken (Biu, 2008). A decade ago, it was reported to have trypanocidal efficacy (Nok et al, 1993). In view of the relative importance of the tree, particularly in its folkloric application in the arid zone of northeastern Nigeria for the treatment of trypanosomosis, this study was therefore, designed to investigate the toxicity and possible anti-trypanosomal effect on Trypanosoma brucei through in vivo and in vitro approach.…”

Antitrypanosomal Potentials of Ethanolic Leaf Extracts of <i>Punica granatum</i> against <i>Trypanosoma brucei brucei</i> Infection

“…Therefore, there is a need to search for cheaper, more effective, easily available and less toxic chemotherapeutic agents for combating trypanosomiasis. The use of herbal preparations for the treatment of the disease still holds a strong potential in that some ethnomedicinal plants have been demonstrated to contain potent trypanocide ( (Nok et al, 1993;.…”