Trypanosoma brucei gambiense Type 1 populations from human patients are clonal and display geographical genetic differentiation

Morrison, Liam J.; Tait, Andy; McCormack, Gillian; Sweeney, Lindsay; Black, Alana; Truc, Philippe; Likeufack, Anne C.L.; Turner, C. Michael R.; MacLeod, Annette

doi:10.1016/j.meegid.2008.08.005

Cited by 59 publications

(55 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The so-called T. brucei brucei 'Kiboko B' (Balmer et al, 2011) appears to be a near-clade too. LD is recorded in T. brucei gambiense (Morrison et al, 2008b) and T. brucei rhodesiense (Duffy et al, 2013). T. brucei gambiense I seems to be strictly clonal (Koffi et al, 2009(Koffi et al, , 2015, a hypothesis that is strongly supported by WGS analysis (Weir et al, 2016).…”

Section: Parasitic Protozoamentioning

confidence: 79%

Is Predominant Clonal Evolution a Common Evolutionary Adaptation to Parasitism in Pathogenic Parasitic Protozoa, Fungi, Bacteria, and Viruses?

Tibayrenc

Ayala

2017

Advances in Parasitology

View full text Add to dashboard Cite

Section: Parasitic Protozoamentioning

confidence: 79%

Is Predominant Clonal Evolution a Common Evolutionary Adaptation to Parasitism in Pathogenic Parasitic Protozoa, Fungi, Bacteria, and Viruses?

Tibayrenc

Ayala

2017

Advances in Parasitology

View full text Add to dashboard Cite

“…One portion was used for DNA extraction using the Qiagen kit, and the other was boiled for supernatant as described previously (34). Tenfold serial dilutions were then prepared from the two DNA stocks, and the supernatant was used to determine the analytical sensitivity of TgsGP LAMP, TgsGP PCR (41), and nested TgsGP PCR (30). The LAMP test was carried out under optimized conditions, and the PCR tests followed the respective published procedures.…”

Section: Methodsmentioning

confidence: 99%

“…The serological test-card agglutination test for trypanosomiasis (CATT)-for T. b. gambiense (24) is widely used; however, the test has varying sensitivities (46) and cannot decisively differentiate between active and cured cases (19). On the molecular side, several PCR tests have been developed (6,30,41), but issues of sensitivity and reproducibility (44) and the requirement for high-precision instrumentation have limited their use.…”

mentioning

confidence: 99%

Detection of Group 1 Trypanosoma brucei gambiense by Loop-Mediated Isothermal Amplification

et al. 2011

View full text Add to dashboard Cite

Trypanosoma brucei gambiense group 1 is the major causative agent of the Gambian human African trypanosomiasis (HAT). Accurate diagnosis of Gambian HAT is still challenged by lack of precise diagnostic methods, low and fluctuating parasitemia, and generally poor services in the areas of endemicity. In this study, we designed a rapid loop-mediated isothermal amplification (LAMP) test for T. b. gambiense based on the 3 end of the T. b. gambiense-specific glycoprotein (TgsGP) gene. The test is specific and amplifies DNA from T. b. gambiense isolates and clinical samples at 62°C within 40 min using a normal water bath. The analytical sensitivity of the TgsGP LAMP was equivalent to 10 trypanosomes/ml using purified DNA and ϳ1 trypanosome/ml using supernatant prepared from boiled blood, while those of classical PCR tests ranged from 10 to 10 3 trypanosomes/ml. There was 100% agreement in the detection of the LAMP product by real-time gel electrophoresis and the DNA-intercalating dye SYBR green I. The LAMP amplicons were unequivocally confirmed through sequencing and analysis of melting curves. The assay was able to amplify parasite DNA from native cerebrospinal fluid (CSF) and double-centrifuged supernatant prepared from boiled buffy coat and bone marrow aspirate. The robustness, superior sensitivity, and ability to inspect results visually through color change indicate the potential of TgsGP LAMP as a future point-of-care test.

show abstract

“…It is clear from the microsatellite studies that T.b. gambiense Type 1 is clonal and very limited, if any, genetic exchange occurs in the field (Morrison et al 2008 ;Koffi et al 2009). …”

Section: P C R-b a S E D M O L E C U L A R E P I D E M I O L O G I C mentioning

confidence: 99%

Molecular epidemiology of African sleeping sickness

Hide

Tait

2009

Parasitology

View full text Add to dashboard Cite

Human sleeping sickness in Africa, caused by Trypanosoma brucei spp. raises a number of questions. Despite the widespread distribution of the tsetse vectors and animal trypanosomiasis, human disease is only found in discrete foci which periodically give rise to epidemics followed by periods of endemicity A key to unravelling this puzzle is a detailed knowledge of the aetiological agents responsible for different patterns of disease--knowledge that is difficult to achieve using traditional microscopy. The science of molecular epidemiology has developed a range of tools which have enabled us to accurately identify taxonomic groups at all levels (species, subspecies, populations, strains and isolates). Using these tools, we can now investigate the genetic interactions within and between populations of Trypanosoma brucei and gain an understanding of the distinction between human- and nonhuman-infective subspecies. In this review, we discuss the development of these tools, their advantages and disadvantages and describe how they have been used to understand parasite genetic diversity, the origin of epidemics, the role of reservoir hosts and the population structure. Using the specific case of T.b. rhodesiense in Uganda, we illustrate how molecular epidemiology has enabled us to construct a more detailed understanding of the origins, generation and dynamics of sleeping sickness epidemics.

show abstract

Trypanosoma brucei gambiense Type 1 populations from human patients are clonal and display geographical genetic differentiation

Cited by 59 publications

References 57 publications

Is Predominant Clonal Evolution a Common Evolutionary Adaptation to Parasitism in Pathogenic Parasitic Protozoa, Fungi, Bacteria, and Viruses?

Is Predominant Clonal Evolution a Common Evolutionary Adaptation to Parasitism in Pathogenic Parasitic Protozoa, Fungi, Bacteria, and Viruses?

Detection of Group 1 Trypanosoma brucei gambiense by Loop-Mediated Isothermal Amplification

Molecular epidemiology of African sleeping sickness

Contact Info

Product

Resources

About