Trypanosoma cruzi: Altered parasites after in vitro treatment with gangliosides, a therapeutic agent in experimental Chagas’ disease

Isasi, S. Cossy; Rodríguez, María Lorena; Pereira, B. M. I.; Díaz-Luján, C.; Fretes, Ricardo; Haüen, D.I.

doi:10.1016/j.exppara.2009.03.017

Cited by 4 publications

(3 citation statements)

References 45 publications

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“…GM1-treated mice survived and recovered with normal frequency. Cardiomyocyte destruction concomitant with the disease was also significantly reduced by a moderate application of GM1 [18,19].…”

Section: Trypanosoma Cruzimentioning

confidence: 81%

“…Cossy-Isasi and collaborators [17] reported that parasite epimastigotes treated with gangliosides from bovine brain presented an altered lipid order that inhibited membrane enzymes and caused morphological alterations. Electron-lucent vacuoles opposite the cytostome, multilamellar bodies and dilated mitochondrion cristae, in addition to a disorganized kinetoplast and altered heterochromatin structure, were found in epimastigote forms [18,19]. Trypomastigotes suffered a loss of cytoplasmic material and organelles when the ganglioside GM1 was present in the culture medium.…”

Section: Trypanosoma Cruzimentioning

confidence: 98%

“…Membrane domains were resistant to treatment with non-ionic detergents at 4 °C. Disruption of the membrane microdomains with methyl--cyclodextrin significantly reduced parasite infectivity suggesting a role of GSLs in macrophage invasion by species of Leishmania [19].…”

Section: Leishmania (L) Amazonensismentioning

confidence: 99%

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Structures of Glycolipids Found in Trypanosomatids: Contribution to Parasite Functions

Barreto‐Bergter¹

2010

TOPARAJ

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Neutral monohexosylceramides (CMHs) globosides (globotriasyl ceramides), other glycosphingolipids (GSLs) and more complex structures such as glycoinositol-phospholipids(GIPLs) and glycosyl phosphatidylinositol (GPI) anchors have been described in several members of the trypanosomatid family. These highly bioactive molecules are not only components of biological structures but also participants in host-parasite interactions such as macrophage invasion, antigenic presentation and signal transduction. Glycolipid structures have been studied using mass spectrometry (MS).This review describes a wide range of glycoconjugates with unique and complex structures that are present in several trypanosomatid species. Their structures are described in the context of their biological significance.

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Section: Trypanosoma Cruzimentioning

confidence: 81%

Section: Trypanosoma Cruzimentioning

confidence: 98%

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Structures of Glycolipids Found in Trypanosomatids: Contribution to Parasite Functions

Barreto‐Bergter¹

2010

TOPARAJ

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New imidazolidine derivatives as anti-Trypanosoma cruzi agents: structure–activity relationships

Gomes¹,

Melo

Peixoto

et al. 2012

Parasitol Res

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Imidazolidine derivatives are key components for the development of bioactive compounds for the treatment of many diseases, especially Chagas. In fact, others studies showed that the imidazolidine-2,4-dione has stood out by presenting a wide spectrum of pharmacological activities including anticonvulsants, antiarrhythmic, and antiparasitic. In the present study, we investigated the morphological alterations induced by imidazolidine derivates LPSF/NN-52 and LPSF/NN-100 on trypomastigotes forms of Trypanosoma cruzi through ultrastructural analysis by electron microscopy. Many concentrations were used to measure the antiparasitic propriety promoted by imidazolidine derivatives, and our study indicates that parasites treated with 13 μg mL(-1) of the imidazolidine derivates for 24 h revealed severe damage to the parasite's mitochondrial complex. Beyond that, also observed in treated parasites were the following: myelin bodies, enlargement of cytoplasm vacuole, fragmentation of endoplasmic reticulum, and some treated samples clearly showed signs of necrosis. To confirm the ultrastructural results, some assays were performed for knowledge cellular death induction promoted by imidazolidine derivates against immune spleen cells. The induction of the necrotic process through derivatives LPSF/NN-52 and LPSF/NN-100 showed similar results in relation to nifurtimox and benznidazole. In the last assays, it was demonstrated that NN-100 was efficient against epimastigotes and trypomastigotes forms and these results reinforce the mechanisms of action of both new imidazolidine derivatives against T. cruzi.

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Immunomodulatory and anti-fibrotic effects of ganglioside therapy on the cardiac chronic form of experimental Trypanosoma cruzi infection

Cutrullis¹,

Poklépovich²,

Postan³

et al. 2011

International Immunopharmacology

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Trypanosoma cruzi: Altered parasites after in vitro treatment with gangliosides, a therapeutic agent in experimental Chagas’ disease

Cited by 4 publications

References 45 publications

Structures of Glycolipids Found in Trypanosomatids: Contribution to Parasite Functions

Structures of Glycolipids Found in Trypanosomatids: Contribution to Parasite Functions

New imidazolidine derivatives as anti-Trypanosoma cruzi agents: structure–activity relationships

Immunomodulatory and anti-fibrotic effects of ganglioside therapy on the cardiac chronic form of experimental Trypanosoma cruzi infection

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