Trypanosoma cruzi: Attachment to perimicrovillar membrane glycoproteins of Rhodnius prolixus

Alves, Carlos Roberto; Albuquerque-Cunha, José M.; Mello, C.B.; Garcia, Elói S.; Nogueira, Nadir F. S.; Bourguingnon, S.C.; Souza, Wanderley de; Azambuja, Patrı́cia; Gonzalez, Marcelo S.

doi:10.1016/j.exppara.2006.11.012

Cited by 70 publications

(90 citation statements)

References 59 publications

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“…This process (epimastigotes change to trypomastigotes forms) is important for vertebrate host infection (Gonzalez et al 2006). Many factors have contributed to the presence of trypanosomes in the gut of the invertebrate host; one of them is the perimicrovillar membrane (PMM), a physical and physiological barrier, which is more evident at 10 days after blood meal and important for T. cruzi development in the insect digestive tract (Nogueira et al 1997;Alves et al 2007).…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo documentation of quantum dots labeled Trypanosoma cruzi–Rhodnius prolixus interaction using confocal microscopy

et al. 2009

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Semiconductor quantum dots (QDs) are highly fluorescent nanocrystals markers that allow long photobleaching and do not destroy the parasites. In this paper, we used fluorescent core shell quantum dots to perform studies of live parasite-vector interaction processes without any observable effect on the vitality of parasites. These nanocrystals were synthesized in aqueous medium and physiological pH, which is very important for monitoring live cells activities, and conjugated with molecules such as lectins to label specific carbohydrates involved on the parasite-vector interaction. These QDs were successfully used for the study of in vitro and in vivo interaction of Trypanosoma cruzi and the triatomine Rhodnius prolixus. These QDs allowed us to acquire real time confocal images sequences of live T. cruzi-R. prolixus interactions for an extended period, causing no damage to the cells. By zooming to the region of interest, we have been able to acquire confocal images at the three to four frames per second rate. Our results show that QDs are physiological fluorescent markers capable to label living parasites and insect vector cells. QDs can be functionalized with lectins to specifically mark surface carbohydrates on perimicrovillar membrane of R. prolixus to follow, visualize, and understand interaction between vectors and its parasites in real-time.

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Section: Introductionmentioning

confidence: 99%

In vitro and in vivo documentation of quantum dots labeled Trypanosoma cruzi–Rhodnius prolixus interaction using confocal microscopy

et al. 2009

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“…The fusion process may be triggered by calcium ionophores [14]. the intestinal cell surface and the perimicrovillar membranes [40]. At the most posterior regions of the intestine and at the rectum many epimastigotes detach from the intestinal surface and transform into trypomastigote forms which are then released together with the feces or with the urine [41].…”

Section: Introductionmentioning

confidence: 99%

Exosomes in the Pathogenic Protozoan Trypanosoma Cruzi

Souza¹

2017

Int J Pathol Clin Res

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Significant advances have recently occurred in the identification and characterization of different types of microvesicles released by eukaryotic cells into the extracellular space. Exosomes are one such type of these vesicles they originate from multivesicular bodies and have received much attention. Here, we review the available data on microvesicles secreted by the pathogenic protozoan Trypanosoma cruzi, which is the causative agent of Chagas disease and that still has a high prevalence in Latin America. The available data show that T. cruzi exosomes contain some key proteins and RNA molecules that contain genetic information and may interfere with transcription. The available information suggests that they play some role in the biology of T. cruzi, including its interaction with host cells, as well as on the pathogenesis of Chagas disease where inflammatory processes and host cell dead occurs as a consequence of parasites, factors released by the parasites, and molecules produced by different host cells that are part of the immunological response.

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“…Also, hydrophobic proteins and carbohydrate residues that bind the Tr. cruzi to peri-microvillar membrane lining mucosal cells have been identified 19 . The study showed significant differences among trypomastigote and amastigote membrane bound molecules such as lipids, carbohydrates and proteins.…”

Section: The Coevolution Of Trypanosomes and Triatominesmentioning

confidence: 99%

“…The full development of peri-microvillar membrane lining mucosal cells of triatomines depends on abdominal distension after a full blood meal, provoking neurosecretion of a prothoracicotrophic hormone 18 . This hormone acts upon prothoracic glands producers of moltinducing ecdisone 19 . Also, hydrophobic proteins and carbohydrate residues that bind the Tr.…”

Section: The Coevolution Of Trypanosomes and Triatominesmentioning

confidence: 99%