1990
DOI: 10.1016/0014-4894(90)90125-v
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Trypanosoma cruzi: Identification of a surface antigen restricted to the flagellar region of the infective form of the parasite

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Cited by 5 publications
(13 citation statements)
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“…The predicted difference in the M r s of the two conceptual TolT proteins cannot account for the observation that MAb 20H1 recognizes four separate protein bands with apparent M r s of 34,000 to 41,000 in Western blots of trypomastigote lysates (33). Since previous studies have shown that these four proteins bind the lectin concanavalin A (33), it seemed possible that the four protein bands detected by MAb 20H1 might represent different glycosylated forms of TolT.…”
Section: Posttranslational Modification Of the Tolt Proteinmentioning
confidence: 93%
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“…The predicted difference in the M r s of the two conceptual TolT proteins cannot account for the observation that MAb 20H1 recognizes four separate protein bands with apparent M r s of 34,000 to 41,000 in Western blots of trypomastigote lysates (33). Since previous studies have shown that these four proteins bind the lectin concanavalin A (33), it seemed possible that the four protein bands detected by MAb 20H1 might represent different glycosylated forms of TolT.…”
Section: Posttranslational Modification Of the Tolt Proteinmentioning
confidence: 93%
“…For analysis of whole-cell lysates, parasites were harvested from culture media by centrifugation, washed twice with phosphate-buffered saline (PBS), and solubilized by the direct addition of boiling 2% sodium dodecyl sulfate (SDS); boiling was continued for 5 min. These and all other samples were separated by one-dimensional polyacrylamide gel electrophoresis (PAGE) and transferred to nitrocellulose filters by previously described methods (33) with a Transblot Cell (Bio-Rad, Richmond, Calif.) overnight at 150 mA. Filters probed with MAb 20H1 were reacted with peroxidase-linked antimouse IgG and detected with an enhanced chemiluminescence system (DuPont, Wilmington, Del.…”
Section: Methodsmentioning
confidence: 99%
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“…In addition, it should be said that the proven success of this 'parental repertoire', along with a shift in R&D priorities converged in curbing the enthusiasm for subsequent large-scale antigen-discovery efforts for Chagas disease. Indeed, most of the additional antigens/biomarkers with moderate-to-excellent diagnostic performance that emerged in the last decades were identified either as by-products of basic hypothesis-driven research (Buchovsky et al, 2001; Di Noia et al, 2002; Martinez et al, 1991; Saborio et al, 1990) or using low-to-medium throughput antigen expression/synthesis approaches (see below). Most importantly, just a few of these novel antigens have been incorporated into the already existing diagnostic application platforms.…”
Section: Diagnostic Applications For Chagas D Isease: Present Knowledgementioning
confidence: 99%
“…Immunofluorescence-Indirect immunofluorescence with preparations of air-dried epimastigotes and trypomastigotes, fixed with acetone at room temperature, was done using FITC-labeled goat anti-mouse as the secondary antibody as described previously (20).…”
Section: Methodsmentioning
confidence: 99%