Trypanosoma cruzi: Two genetic groups in Paraná state, Southern Brazil

Zalloum, Leila; Gomes, Mônica Lúcia; Kinoshita, Andréia Tieko; Toledo, M. J. O.; Prioli, Alberto José; Araújo, Silvana Márques de

doi:10.1016/j.exppara.2005.05.006

Cited by 15 publications

(10 citation statements)

References 17 publications

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“…These analyses indicated the concomitant presence of populations of two principal lineages, T. cruzi I and II, with a predominance of T. cruzi I, as initially suggested by the RAPD data. These data are in agreement with recent findings that T. cruzi I and II circulate in north/northwest Paraná (Zalloum et al 2005). These results also indicate that the strains analyzed here are more closely associated with the sylvatic transmission cycle of T. cruzi in this region, as reported for isolates from the sylvatic cycle in the most diverse endemic areas of Brazil and other countries of southern South America (Fernandes et al 1997, Andrade 1999, Devera et al 2003.…”

Section: Discussionsupporting

confidence: 82%

“…In general, the majority of the strains formed a closely related genetic group and could not be assigned to subgroups, either according to the hosts from which they were isolated, or their geographical origin. This genetic similarity detected by the RAPD is in accord with the results of Zalloum et al (2005), who studied strains isolated from triatomines and sylvatic reservoirs in the same region. Limited genetic variability was also observed by Soccol et al (2002), who analyzed, through isoenzymes, isolates of T. cruzi from two distinct regions of the state of Paraná, and by Carrasco et al (1996), who observed homogeneous patterns of RAPD among strains of T. cruzi isolated from sylvatic reservoirs in different geographical areas of South and Central America.…”

Section: Discussionsupporting

confidence: 76%

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Trypanosoma cruzi-triatomine associations and the presence of mixed infections in single triatomine bugs in Paraná state, Brazil

Spitzner

Freitas

Macedo

et al. 2007

Acta Parasitologica

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Eighteen strains of Trypanosoma cruzi isolated from two species of triatomines in the state of Paraná, Brazil, were characterized molecularly using three strategies: RAPD (randomly amplified polymorphic DNA) with four primers, analysis of the D7 polymorphic region of the 24Sα rDNA, and RFLP (restriction fragment length polymorphism) of region 5' of the mitochondrial gene COII (cytochrome oxidase subunit 2). The phenogram constructed with the RAPD data showed that only three strains isolated from Panstrongylus megistus collected in the Municipality of Arapongas were grouped together in a sub-branch. None of the other 15 strains could be clustered according to triatomine species or geographical origin. The strains were grouped with the T. cruzi I reference sample, indicating closer association with the sylvatic transmission cycle of T. cruzi in the state of Paraná. However, analyses of the rDNA and COII gene polymorphisms revealed the presence of populations from both T. cruzi I and II major lineages. In half of the analyzed triatomines, we found parasites from both lineages coinfecting the same bugs. Of these, most (6/9) were isolated from Triatoma sordida, and 3/9 from Panstrongylus megistus. These results contribute to a better comprehension of the ecoepidemiology of Chagas' disease in Paraná, and raise questions about the role of studies of polyclonal population dynamics for controlling the transmission of T. cruzi to humans in this region.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 76%

Trypanosoma cruzi-triatomine associations and the presence of mixed infections in single triatomine bugs in Paraná state, Brazil

Spitzner

Freitas

Macedo

et al. 2007

Acta Parasitologica

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“…Table 1 shows the genetic groups, hosts, and isolation methods for the 12 strains used. These strains were recently isolated and characterized by Zalloum et al (2005) using randomly amplified polymorphic DNA (RAPD) and simple sequence repeat-anchored PCR (SSR-PCR). They were then divided into two genetic groups: T. cruzi I and II.…”

Section: Introductionmentioning

confidence: 99%

Infectivity for mice of Trypanosoma cruzi I and II strains isolated from different hosts

et al. 2006

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In this paper, the infectivity for mice of Trypanosoma cruzi I and II strains isolated from sylvatic animals, triatomines, and humans is determined using fresh blood examination, hemoculture, culture of macerated organs, and polymerase chain reaction (PCR). Six strains were considered to have low infectivity (9.1-18.2%), five medium (27.3-45.4%), and one high (100.0%). Infectivity of T. cruzi strains isolated from sylvatic animals was significantly higher than that of strains isolated from humans and triatomines (p=0.0141). No significant difference was observed between the infectivity of T. cruzi I and II strains. The parasite was detected by fresh blood examination in one strain, by hemoculture and culture of macerated organs in four strains, and by PCR in all strains. We conclude that the infectivity is related to the host from which the strains were isolated, but the infectivity is not related to the genetic group of the parasite. We also conclude that the majority of the strains studied have low and medium infectivity for mice, and that PCR is an important tool to detect T. cruzi in strains with this biological characteristic.

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“…Although the present consensus is to refer to six DTUs (TcI-VI) for these strains 52 , many studies have investigated the genetic-biological relationship in terms of virulence in mice, transmissibility by triatomines, infectivity to cell cultures, and drug sensitivity in vitro and in vivo, using populations belonging to the two lineages that were formerly distinguished as T. cruzi I and II 23,24,30,39,46 . Populations of T. cruzi from northern and northwestern areas of the state of Paraná, Brazil have been determined to belong to these two major genetic lineages 51 . Strains isolated from triatomines and wild reservoirs belong to T. cruzi I, and isolates from humans belong to T. cruzi II.…”

mentioning

confidence: 99%

Induction of phagocytic activity and nitric-oxide production in natural populations of Trypanosoma Cruzi I and II from the state of Paraná, Brazil

Zalloum

Lala

Moreira

et al. 2011

Rev. Inst. Med. trop. S. Paulo

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SUMMARYTwelve strains of Trypanosoma cruzi isolated from wild reservoirs, triatomines, and chronic chagasic patients in the state of Paraná, southern Brazil, and classified as T. cruzi I and II, were used to test the correlation between genetic and biological diversity. The Phagocytic Index (PI) and nitric-oxide (NO) production in vitro were used as biological parameters. The PI of the T. cruzi I and II strains did not differ significantly, nor did the PI of the T. cruzi strains isolated from humans, triatomines, or wild reservoirs. There was a statistical difference in the inhibition of NO production between T. cruzi I and II and between parasites isolated from humans and the strains isolated from triatomines and wild reservoirs, but there was no correlation between genetics and biology when the strains were analyzed independently of the lineages or hosts from which the strains were isolated. There were significant correlations for Randomly Amplified Polymorphic Deoxyribonucleic acid (RAPD) and biological parameters for T. cruzi I and II, and for humans or wild reservoirs when the lineages or hosts were considered individually.

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Trypanosoma cruzi: Two genetic groups in Paraná state, Southern Brazil

Cited by 15 publications

References 17 publications

Trypanosoma cruzi-triatomine associations and the presence of mixed infections in single triatomine bugs in Paraná state, Brazil

Trypanosoma cruzi-triatomine associations and the presence of mixed infections in single triatomine bugs in Paraná state, Brazil

Infectivity for mice of Trypanosoma cruzi I and II strains isolated from different hosts

Induction of phagocytic activity and nitric-oxide production in natural populations of Trypanosoma Cruzi I and II from the state of Paraná, Brazil

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