Trypanosoma Species (American Trypanosomiasis, Chagas' Disease)

Kirchhoff, Louis V.

doi:10.1016/b978-0-443-06839-3.00277-0

Cited by 14 publications

(37 citation statements)

References 140 publications

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“…The persistence of parasites in heart muscle, as amastigote forms, stimulates a chronic inflammatory process that often results in rhythm disturbances and cardiomyopathy. 6 Heart transplantation for end-stage chronic Chagas' heart disease was first reported in Brazil in 1985 at the Heart Institute (InCor), University of São Paulo Medical School. This disease represents the third leading cause of heart transplantation in the country, following ischemic and dilated cardiomyopathy.…”

mentioning

confidence: 99%

Risk Factors for Chagas' Disease Reactivation After Heart Transplantation

Campos

Strabelli

Neto

et al. 2008

The Journal of Heart and Lung Transplantation

146

122

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mentioning

confidence: 99%

Risk Factors for Chagas' Disease Reactivation After Heart Transplantation

Campos

Strabelli

Neto

et al. 2008

The Journal of Heart and Lung Transplantation

146

122

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“…Der Genus Trypanosoma besteht aus etwa 20 Spezies, von denen 2 oder 3 humanpathogen sind [55]. [82].…”

Section: B 1 Wissensstand üBer Den Erregerunclassified

Arboprotozoen

2009

Bundesgesundheitsbl.

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“…RIPAs were performed by David Leiby at the ARC, Rockville, MD (19), using three negative and three positive control samples. Briefly, the assay detects two T. cruzi-specific glycoproteins of 72 and 90 kDa identified in radioautographs after radioimmunoprecipitation with T. cruzi-positive sera (17,18). Where possible, the radioautographs were graded by their intensity.…”

Section: Methodsmentioning

confidence: 99%

Lateral Flow Immunoassay for Diagnosis of Trypanosoma cruzi Infection with High Correlation to the Radioimmunoprecipitation Assay

Houghton

Stevens

Hjerrild

et al. 2009

Clin Vaccine Immunol

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The incidence of blood donors seropositive for Trypanosoma cruzi in North America has increased with population migration and more rigorous surveillance. The United States, considered nonendemic for T. cruzi, could therefore be at risk to exposure to parasite transmission through blood or organ donations. Current tests show variable reactivity, especially with Central American sera. Here we describe the development of a lateral flow immunoassay for the rapid detection of T. cruzi infection that has a strong correlation to the radioimmunoprecipitation assay (RIPA) "gold standard" in the United States. Such a test could have utility in small blood banks for prescreening donors, as well as in cardiac transplantation evaluation. T. cruzi consensus and/or RIPA-positive sera from Central and South America were evaluated in enzyme immunoassays (EIAs). These included commercial panels from Boston Biomedica, Inc. (BBI) (n ‫؍‬ 14), and HemaBio (n ‫؍‬ 21). Other sources included RIPA-positive sera from the American Red Cross (ARC) (n ‫؍‬ 42), as well as from Chile. Sera were tested with the multiepitope recombinant TcF. All but one of the BBI samples were positive and 7 of 21 HemaBio samples and 6 of 42 ARC samples were low positive or negative. This observation indicated the need for additional antigens. To complement TcF reactivity, we tested the sera with peptides 30, 36, SAPA, and 1.1, 1.2, and 1.3 His fragments of 85-kDa trans-sialidase. We identified a promising combination of the tested antigens and constructed a single recombinant protein, ITC6, that enhanced the relative sensitivity in U.S. blood donor sera compared to that of TcF. The data on its evaluation using RIPA-confirmed positive sera in EIA and lateral flow immunoassay studies are presented, along with an additional recombinant protein, ITC8.2, with two additional sequences for peptide 1 and Kmp-11. The latter, when evaluated in a dipstick assay with consensus positive sera, had a sensitivity of 99.2% and a specificity of 99.1%.

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Trypanosoma Species (American Trypanosomiasis, Chagas' Disease)

Cited by 14 publications

References 140 publications

Risk Factors for Chagas' Disease Reactivation After Heart Transplantation

Risk Factors for Chagas' Disease Reactivation After Heart Transplantation

Arboprotozoen

Lateral Flow Immunoassay for Diagnosis of Trypanosoma cruzi Infection with High Correlation to the Radioimmunoprecipitation Assay

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