Trypanosoma vivax infection in dairy cattle: Parasitological and serological diagnosis and its relationship with the percentage of red blood cells

Silva, Aleksandro S. Da; Molosse, Vitor L.; Deolindo, Guilherme Luiz; Cécere, Bruno Giorgio de Oliveira; Vitt, Maksuel Gatto de; Nascimento, Luiz Flávio Nepomuceno do; Neves, Gabriella Bassi das; Sartor, Josenei; Sartori, Vitor H.; Baldissera, Matheus D.; Miletti, Luiz Cláudio

doi:10.1016/j.micpath.2022.105495

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…Territorial proximity facilitates pathogen dispersion from different locations, which is an epidemiologically important factor because western RS borders Santa Catarina state and Argentina, where T. vivax was detected in cattle ( Paoletta et al, 2018 ; Silva et al, 2022 ). Some serological studies using symptomatic cattle from the western region of Santa Catarina detected 39.0% (57/146) seropositive animals, suggesting T. vivax existence in these areas.…”

Section: Discussionmentioning

confidence: 99%

Detection of anti-Trypanosoma spp. antibodies in cattle from southern Brazil

Samoel,

Fernandes,

Roman

et al. 2024

Rev. Bras. Parasitol. Vet.

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Bovine trypanosomosis, caused by Trypanosoma vivax, is a disease that originated in Africa and currently affects cattle in several South American countries, including almost all Brazilian states. Despite the reports on T. vivax infection in southern Brazil, data on its circulation status is currently unavailable. In this study, we aimed to detect anti-Trypanosoma spp. IgG antibodies in cattle from Rio Grande do Sul and suggest areas with T. vivax transmission risk. A total of 691 serum samples from cattle in the intermediate regions of Rio Grande do Sul were analyzed using indirect immunofluorescence assay (IFA). The overall seroprevalence of anti-Trypanosoma antibodies in cattle was 24.6% (170/691). The detection rate ranged from 0-37.3%, with a high prevalence in the intermediate regions of Ijuí (37.3%), Uruguaiana (30.7%), and Passo Fundo (28.9%). Thus, these regions were suggested as possible bovine trypanosomosis risk areas due to the high seroprevalence. This is the first serological study to determine Trypanosoma spp. infection status in cattle from Rio Grande do Sul, providing data on the epidemiology of trypanosomosis in the state.

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Section: Discussionmentioning

confidence: 99%

Detection of anti-Trypanosoma spp. antibodies in cattle from southern Brazil

Samoel,

Fernandes,

Roman

et al. 2024

Rev. Bras. Parasitol. Vet.

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Examination of Bovine Red Blood Cell Death in Vitro in Response to Pathophysiologic Proapoptotic Stimuli

Kennedy,

Mirza,

Mandiangu

et al. 2023

Front. Biosci. (Landmark Ed)

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Background: Interspecies variations in mammalian red blood cells (RBCs) are observed in circulating RBC lifespan, cell size, fluidity, aggregation, water permeability, metabolism, lipid composition, and the overall proteome. Bovine RBC cell membrane is deficient in phosphatidylcholine and exhibits anomalies in the arrangement of phosphatidylethanolamine within the lipid bilayer. However, like human RBCs, virtually all the aminophospholipid phosphatidylserine (PS) is found within the cytoplasmic side of the cell membrane of intact circulating bovine RBCs. During apoptotic cell death of human and murine RBCs, PS translocates to the outer leaflet of the cell membrane via Ca2+-dependent and -independent signaling mechanisms. However, little is known about this process in bovine RBCs. Methods: Using cytofluorometry analyses, we characterized and compared the cell death responses in bovine and human RBCs in vitro exposed to various pathophysiologic cell stressors. Results: Ionic stress, by ionophore treatment, and oxidative stress enhanced cytoplasmic Ca2+ levels and cell membrane PS expression in both bovine and human RBCs. Fever-grade hyperthermia and energy starvation promoted Ca2+ influx and elevated reactive oxygen species levels in both human and bovine RBCs. However, bovine RBCs displayed minimal increases in PS expression elicited by hyperthermia, energy starvation, and extracellular hypertonicity as compared to human RBCs. In response to decreased extracellular osmolality, bovine RBCs exhibited significantly enhanced fragility as compared to human RBCs. Conclusions: Bovine RBCs display differential cell death patterns as compared to human RBCs, only partly explained by increased Ca2+ influx and oxidative stress. Premature removal of circulating RBCs could potentially contribute to the pathogenesis of anemia in cattle caused by a wide range of factors such as systemic diseases, parasitic infections, and nutritional deficiencies.

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Trypanosoma vivax infection in dairy cattle: Parasitological and serological diagnosis and its relationship with the percentage of red blood cells

Cited by 2 publications

References 15 publications

Detection of anti-Trypanosoma spp. antibodies in cattle from southern Brazil

Detection of anti-Trypanosoma spp. antibodies in cattle from southern Brazil

Examination of Bovine Red Blood Cell Death in Vitro in Response to Pathophysiologic Proapoptotic Stimuli

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