1998
DOI: 10.1006/abbi.1998.0723
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Trypsin Cleavage of Human Cystathionine β-Synthase into an Evolutionarily Conserved Active Core: Structural and Functional Consequences

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Cited by 151 publications
(215 citation statements)
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“…Comparison of spectroscopic properties of the purified wild type and the 1-413 deletion mutant confirmed the previous observation [21] that removal of the C-terminal regulatory region, spanning amino acids 414-551, does not impair heme binding (results not shown). However, the 71-400 deletion mutant, which is missing both the 70 amino acid N-terminal heme-binding domain and the entire C-terminal regulatory region cannot bind heme, which is illustrated by the lack of the Soret peak at 428 nm in its absorption profile [11].…”
Section: Spectroscopic Characterization Of the Wild Type 1-413 And 7supporting
confidence: 87%
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“…Comparison of spectroscopic properties of the purified wild type and the 1-413 deletion mutant confirmed the previous observation [21] that removal of the C-terminal regulatory region, spanning amino acids 414-551, does not impair heme binding (results not shown). However, the 71-400 deletion mutant, which is missing both the 70 amino acid N-terminal heme-binding domain and the entire C-terminal regulatory region cannot bind heme, which is illustrated by the lack of the Soret peak at 428 nm in its absorption profile [11].…”
Section: Spectroscopic Characterization Of the Wild Type 1-413 And 7supporting
confidence: 87%
“…The measured UV maximum at 280 nm for the wild type enzyme was 83.75 mM −1 cm −1 , which is much higher than the calculated theoretical value of 57.08 mM −1 cm −1 . This phenomenon has been observed previously [21] and attributed to the significant contribution of the heme and PLP cofactors to the UV absorbance [21]. Truncation of the wild type CBS by 138 C-terminal amino acids (CBS 1-413) removes five phenylalanine residues and one tyrosine, but has no effect on the heme and PLP content when compared to its wild type counterpart.…”
Section: Spectroscopic Characterization Of the Wild Type 1-413 And 7supporting
confidence: 56%
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“…Mutation C275Y lies within the CPGC motif (residues 272-275) of mammalian CBS enzymes, involved in redox reactions [Meier et al, 2001]. Mutation L456P, which affects a leucine residue conserved among mammalian CBS enzymes, lies in the CBS domain [Bateman, 1997], within the C-terminal regulatory region [Kery et al, 1998], which is involved in AdoMet activation.…”
Section: ………Rcvvilpdsvrnymskflsdk………………………………apvvn-e----sgailgmvtmentioning
confidence: 99%