Trypsin immobilization by direct adsorption on metal ion chelated macroporous chitosan-silica gel beads

Interaction and complex formation between catalase and cationic polyelectrolytes: Chitosan and Eudragit E100

“…to chain repulsion, diffusion of proton and counter ions together with water inside the gel and dissociation of secondary interactions. Due to its positively charged amine groups, chitosan and Eudragit E100 interact with negatively charged molecules through coulombic forces [3,4]. If the negatively charged molecules are proteins, non-soluble complexes are formed which can be separated from the other macromolecules by simple precipitation.…”

Preparing a metal-ion chelated immobilized enzyme reactor based on the polyacrylamide monolith grafted with polyethylenimine for a facile regeneration and high throughput tryptic digestion in proteomics

“…reversibly by the coordination role between amino acids [22] and metal-ions. Secondly, the metal-ion bonded with enzyme could be immobilized reversibly onto support materials.…”

“…Grafting a hydrophilic and functional macromolecule polymer on support [22][23][24][25] is extensively used in the biochemistry field to accomplish enzyme immobilization reversibly. For instance, Wu et al [22] prepared a kind of silica gel bead coated with macroporous chitosan layer.…”

“…For instance, Wu et al [22] prepared a kind of silica gel bead coated with macroporous chitosan layer. As a result, the metal immobilized affinity adsorbents could be obtained by chelating Cu 2+ , and trypsin was immobilized subsequently.…”

Preparation and chelation adsorption property of composite chelating material poly(amidoxime)/SiO2 towards heavy metal ions

“…For preparing these composite chelating materials, two methods can be adopted. One is coating or impregnating the inorganic particles with the solutions of chelating polymers [13][14][15], and this is a conventional and simple method; another method is to try to chemically bond the polymer with chelating function onto the surfaces of the inorganic particles (chemically anchoring or grafting) [16]. This method is more effective due to the combining stability of the polymers and inorganic particles, and should be developed vigorously.…”