Trypsin preservation: CsUGT91C1 regulates Trilobatin Biosynthesis in Cucumis sativus during Storage

Wang, Jie; Jia, Jingyu; Sun, Jiaju; Pang, Xinyue; Li, Bairu; Yuan, Jin-Peng; Chen, Enyan; Li, Xin

doi:10.1007/s10725-023-00962-w

Cited by 4 publications

(2 citation statements)

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“…In this sense, the synthesis of flavonols, flavonoids, and phenylpropanoids has been proposed in D. antarctica as a way to increase the antioxidant capacity of cold-acclimated plants [ 39 ]. Our results confirm that ‘flavonol 4′-sulfotransferase’ and various ‘UDP-glycosyltransferase’ genes (UGT73B3, UGT73C1, UGT73C5, UGT91C1) that encode enzymes related to these antioxidant molecules synthesis pathways [ 53 , 54 , 55 , 56 , 57 ] were co-expressed together in freezing tolerant plants (CA and DW+), while their expression was down-regulated during cold deacclimation as result of nocturnal warming.…”

Section: Discussionsupporting

confidence: 80%

Transcriptome Analysis of Diurnal and Nocturnal-Warmed Plants, the Molecular Mechanism Underlying Cold Deacclimation Response in Deschampsia antarctica

López

Larama

Sáez

et al. 2023

IJMS

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Warming in the Antarctic Peninsula is one of the fastest on earth, and is predicted to become more asymmetric in the near future. Warming has already favored the growth and reproduction of Antarctic plant species, leading to a decrease in their freezing tolerance (deacclimation). Evidence regarding the effects of diurnal and nocturnal warming on freezing tolerance-related gene expression in D. antarctica is negligible. We hypothesized that freezing tolerance-related gene (such as CBF-regulon) expression is reduced mainly by nocturnal warming rather than diurnal temperature changes in D. antarctica. The present work aimed to determine the effects of diurnal and nocturnal warming on cold deacclimation and its associated gene expression in D. antarctica, under laboratory conditions. Fully cold-acclimated plants (8 °C/0 °C), with 16h/8h thermoperiod and photoperiod duration, were assigned to four treatments for 14 days: one control (8 °C/0 °C) and three with different warming conditions (diurnal (14 °C/0 °C), nocturnal (8 °C/6 °C), and diurnal-nocturnal (14 °C/6 °C). RNA-seq was performed and differential gene expression was analyzed. Nocturnal warming significantly down-regulated the CBF transcription factors expression and associated cold stress response genes and up-regulated photosynthetic and growth promotion genes. Consequently, nocturnal warming has a greater effect than diurnal warming on the cold deacclimation process in D. antarctica. The eco-physiological implications are discussed.

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Section: Discussionsupporting

confidence: 80%

Transcriptome Analysis of Diurnal and Nocturnal-Warmed Plants, the Molecular Mechanism Underlying Cold Deacclimation Response in Deschampsia antarctica

López

Larama

Sáez

et al. 2023

IJMS

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“…The primer sequences could be found in Table S1. Concurrently, the weight loss rate was periodically assessed and recorded throughout the experimental duration [34].…”

Section: Rt-qpcr and Determination Of Indexesmentioning

confidence: 99%

CsWRKY33: A Crucial Transcription Factor in the MAPK Pathway Inhibited by Trypsin, Participating in the Suppression of Cucumis sativus Senescence

Sun,

wang,

Chen

et al. 2023

Preprint

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Background The Mitogen-Activated Protein Kinase (MAPK) cascade pathway extensively participates in the physiological regulation processes of plants. Trypsin, as an inducer of resistance, significantly enhanced the storage quality of Cucumis sativus (C. sativus). However, the mechanism by which trypsin regulated the synthesis of phenylpropanoid compounds through the MAPK pathway to influence fruit resistance remained to be elucidated. Methods To investigate the molecular mechanism underlying trypsin-induced C. sativus resistance, we conducted a combined transcriptomic and widely targeted metabolomic analysis, validated through Virus-Induced Gene Silencing (VIGS). Results Transcriptomic results revealed that a total of 83 differentially expressed genes (DEGs)were enriched in the MAPK pathway, with 48 genes significantly downregulated and 35 genes significantly upregulated. GSEA analysis further identified the WRKY33 transcription factor from the leading edge subset. Bioinformatics analysis indicated that CsWRKY33 shared high homology with WRKY22 in Arabidopsis. The combined analysis of transcriptomics and widely targeted metabolomics demonstrated significant upregulation in the synthesis of compounds such as vanillin, dihydrocharcone-4'-O-glucoside, and 2-hydroxy-3-phenylpropanoic acid. Co-expression network analysis showed that these key metabolites were negatively regulated by CsWRKY33. VIGS results showed that silencing CsWRKY33 enhanced fruit resistance and extended storage time. Conclusion This study revealed that trypsin could downregulate the expression of CsWRKY33 and promote the synthesis of compounds with high antioxidant and antibacterial activity, such as vanillin, dihydrocharcone-4'-O-glucoside, and 2-hydroxy-3-phenylpropanoic acid. This enhancement led to increasing fruit disease resistance and delayed senescence and decay.

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Screening Key Genes Related to Nitrogen Use Efficiency in Cucumber Through Weighted Gene Co-Expression Network Analysis

Ma,

Wei,

Liu

et al. 2024

Genes

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Cucumber (Cucumis sativus L.) is a crucial vegetable crop, requiring significant nitrogen fertilizer inputs. However, excessive nitrogen application not only impairs growth but also poses severe environmental risks. Thus, enhancing nitrogen use efficiency (NUE) in cucumber is imperative. For the identification of genes associated with NUE in cucumber, roots of high NUE and low NUE lines were analyzed under high nitrogen conditions. Using transcriptome sequencing through WGCNA, a total of 15,180 genes were categorized into 35 co-expression modules, with 5 modules being highly correlated with NUE. Based on differential expression within the five modules and the results of GO and KEGG enrichment analyses, 25 genes were identified as potentially related to NUE. Among these, CsaV4_1G002492 (GLR22), CsaV4_2G003460 (GLR35), CsaV4_3G000307 (NRT1.1), and CsaV4_7G001709 (UPS2) were homologous to genes in Arabidopsis known to directly participate in NUE related process. These four genes were chosen as key genes for further analysis. qRT-PCR analysis revealed that CsaV4_3G000307 and CsaV4_7G001709 were more active during the early stages of the high nitrogen treatment in the high NUE line. Conversely, CsaV4_1G002492 and CsaV4_2G003460 were more active in the low NUE line. Using transcriptomic analysis, a frameshift INDEL mutation was observed in CsaV4_3G000307 in the low NUE line, which impacted the compactness of the protein structure, potentially altering its function. Analysis of protein interactions of these four key genes predicted some potential interaction networks. This research offers critical insights into the genetic factors influencing NUE in cucumber, presenting potential targets for genetic modification or breeding programs.

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Trypsin preservation: CsUGT91C1 regulates Trilobatin Biosynthesis in Cucumis sativus during Storage

Cited by 4 publications

References 42 publications

Transcriptome Analysis of Diurnal and Nocturnal-Warmed Plants, the Molecular Mechanism Underlying Cold Deacclimation Response in Deschampsia antarctica

Transcriptome Analysis of Diurnal and Nocturnal-Warmed Plants, the Molecular Mechanism Underlying Cold Deacclimation Response in Deschampsia antarctica

CsWRKY33: A Crucial Transcription Factor in the MAPK Pathway Inhibited by Trypsin, Participating in the Suppression of Cucumis sativus Senescence

Screening Key Genes Related to Nitrogen Use Efficiency in Cucumber Through Weighted Gene Co-Expression Network Analysis

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