Tryptase and chymase are angiogenic in vivo in the chorioallantoic membrane assay

Ribatti, Doménico; Ranieri, Girolamo; Benagiano, Vincenzo; Crivellato, Enrico

doi:10.1387/ijdb.103138dr

Cited by 63 publications

(46 citation statements)

References 39 publications

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“…MMP-9, Collagenase, Gelatinase, Elastase (Fang et al, 1999;Bao Dang et al, 2013), MPO (Baldus et al, 2001;Shechter et al, 2011) Elastase (Kitson et al, 1998;Jacobsen et al, 2007) Tryptase Chymase (Weerth et al, 2003;Tchougounova et al, 2005), MMP-2, MMP-9 (Fang et al, 1999;Stirling et al, 2009) (Rahpeymai et al, 2006;Hao et al, 2010), BDNF, NT-3 (Hammarberg, et al 2000;Shinjyo et al, 2009) Sema4A, NT-3 (Bénard et al, 2008;Ishii et al, 2012) NAA (modulation of H 2 O 2 -induced apoptosis) (Warrington et al, 2004;Mantovani et al, 2011) Angiogenesis C5 (Norrby, 2002;Langer et al, 2010) VEGF (Scapini et al, 2004;Ribatti et al, 2011), MMP-9 (Justicia et al, 2003;Riboldi et al, 2005) PDGF, TNF-α and VEGF (Horiuchi and Weller, 1997) VEGF, TNF-α and β, FGF, MMP (Norrby et al, 2002) tryptase and chymase (Ribatti et al, 2011) VEGF (Riboldi et al, 2005), TNF-α, transdifferentiation in ELCs (Fernandez Pujol et al, 2001), trogocytosis of VEGF receptor …”

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confidence: 99%

The role of the immune system in central nervous system plasticity after acute injury

Peruzzotti-Jametti¹,

Donegá²,

Giusto³

et al. 2014

Neuroscience

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Acute brain injuries cause rapid cell death that activates bidirectional crosstalks between the injured brain and the immune system.In the acute phase, the damaged central nervous system (CNS) activates resident and circulating immune cells via the local and systemic release of soluble mediators. This early immune activation is necessary to confine the injured tissue and foster the clearance of cellular debris, which would ultimately bring the inflammatory reaction to a close. In the chronic phase, a sustained immune activation is described in many CNS disorders, and the degree of this prolonged response has variable effects on the spontaneous brain regenerative processes. The challenge for treating acute CNS damages is to understand how to optimally engage and modify these immune responses, thus providing new strategies that will compensate for tissue lost to injury.Here we have reviewed the available information about the role and function of the innate and adaptive immune responses in influencing CNS plasticity during the acute and chronic phases of recovery after injury. We have examined how CNS damage evolves along the activation of main Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Europe PMC Funders Group

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mentioning

confidence: 99%

The role of the immune system in central nervous system plasticity after acute injury

Peruzzotti-Jametti¹,

Donegá²,

Giusto³

et al. 2014

Neuroscience

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“…Using in ovo formalin-fixed specimens and histochemistry followed by microscopic evaluation, we demonstrated a highly angiotropic effect of mast cells early after inoculation. Although several studies have reported the angiogenic effects of mast cells and/or their granules on chick embryo chorioallantoic membrane (6,24,25), the angiogenic mechanism by which mast cells induce the development of new blood vessels in the chick embryo chorioallantoic membrane has not been clearly described. Evidence from our study suggests a complex mechanism of mast cell-induced angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Cultured tumor cells were washed in Dulbecco's PBS (DPBS) and 10 6 living cells in 100 mL PBS were inoculated below the chorioallantoic membrane. Inoculation was performed in a blood vessel-free area of the chick embryo chorioallantoic membrane to avoid hemorrhagic events.…”

Section: Cell Inoculation and Experiments Monitoringmentioning

confidence: 99%

“…Mast cells exert distinct non-immunological functions, being involved in tissue homeostasis, fibrosis and angiogenesis. The involvement of mast cells in angiogenesis has been strongly supported by clinico-pathological (3)(4)(5) and experimental studies (6,7) in different malignant and non-malignant diseases, but their role in the angiogenic process is still not well characterized. The involvement of mast cells in angiogenesis is supported by the ability of these cells to secrete growth factors and molecules that induce vascular hyperpermeability (8).…”

Section: Introductionmentioning

confidence: 99%

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Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryos

Avram¹,

Cimpean²,

Raica³

2013

Braz. J. Med. Biol. Res.

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The P1.HTR cell line includes highly transfectable cells derived from P815 mastocytoma cells originating from mouse breast tissue. Despite its widespread use in immunogenic studies, no data are available about the behavior of P1.HTR cells in the chick embryo chorioallantoic membrane model. The objective of the present investigation was to study the effects of P1.HTR cells implanted on the chorioallantoic membrane of chick embryos. We inoculated P1.HTR cells into the previously prepared chick embryo chorioallantoic membrane and observed the early and late effects of these cells by stereomicroscopy, histochemistry and immunohistochemistry. A highly angiotropic and angiogenic effect occurred early after inoculation and a tumorigenic potential with the development of mastocytoma keeping well mast cells immunophenotype was detected later during the development. The P1.HTR mastocytoma cell line is a good tool for the development of the chick embryo chorioallantoic membrane mastocytoma model and also for other studies concerning the involvement of blood vessels. The chick embryo chorioallantoic membrane model of mastocytoma retains the mast cell immunophenotype under experimental conditions and could be used as an experimental tool for in vivo preliminary testing of antitumor and antivascular drugs.

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“…Notably, experimental studies showed the release of tryptase from mast cells to be a potent angiogenic factor, able to stimulate the neovascolarization both in vitro (16) and in vivo (17), and therefore directly involved in cancer cell proliferation. Consequently, human tryptase induces vascular tube formation either by direct mitogen action on endothelial cells (18)(19)(20)(21) or by indirect proteolytic action on the surrounding matrix (22)(23)(24).…”

Section: Introductionmentioning

confidence: 99%

Tryptase serum levels in patients suffering from hepatocellular carcinoma undergoing intra-arterial chemoembolization: Possible predictive role of response to treatment

Goffredo

Gadaleta

Laterza

et al. 2013

Molecular and Clinical Oncology

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Abstract. Tryptase is a serin protease stored in mast cell granules that has recently been found to be involved in tumor angiogenesis. Data from experimental tumor models have suggested that prior to the onset of angiogenesis mast cells were accumulated near tumor cells and were required for the macroscopic expansion and metastatic spread of primary tumor cells. Hepatocellular cancer (HCC) is a well-established, highly angiogenesis-dependent hypervascular tumor. The aim of this preliminary study was to assess tryptase serum levels in 30 HCC patients prior and subsequent to hepatic transarterial chemoembolization (TACE). In this study, patients with intermediate stage (B) HCC, according to the Barcelona Clinic Liver Cancer (BCLC) staging classification, were enrolled. Additional patient features were adequate liver functional reserve and A or B status, according to the Child-Pugh classification. Tryptase levels were measured using the UniCAP-Tryptase fluoroimmunoassay. TACE was performed by loading doxorubicin on microspheres. The mean ± standard deviation (SD) tryptase level pre-TACE was 7.74±3.62 µg/l, and post-TACE 4.67±2.79 µg/l. A statistically significant difference (P<0.001) was detected, using the Student's t-test, between pre-and post-TACE tryptase level concentrations. No correlations were found between tryptase levels and other important clinicopathological features of patients. This is the first preliminary study analyzing the potential significance of serum tryptase levels in HCC patients. The results demonstrated higher serum tryptase levels in HCC patients, suggesting tryptase release from HCC tissue. As expected, after TACE, serum tryptase levels were decreased. Therefore, we suggested that tryptase was a potential biomarker of response to TACE treatment in HCC patients.

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Tryptase and chymase are angiogenic in vivo in the chorioallantoic membrane assay

Cited by 63 publications

References 39 publications

The role of the immune system in central nervous system plasticity after acute injury

The role of the immune system in central nervous system plasticity after acute injury

Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryos

Tryptase serum levels in patients suffering from hepatocellular carcinoma undergoing intra-arterial chemoembolization: Possible predictive role of response to treatment

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