Tryptase concentrations in bronchoalveolar lavage from patients with chronic eosinophilic pneumonia

Bargagli, Elena; Bigliazzi, Caterina; Leonini, Alessandra; Nikiforakis, Nikolaos; Perari, M. G.; Rottoli, Paola

doi:10.1042/cs20040178

Cited by 17 publications

(16 citation statements)

References 19 publications

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“…In addition, CXCL9, CXCL10, CCL5 (RANTES), CCL11 (eotaxin-1), CCL2, and CCL4 have also been observed to be elevated in CEP (73,75,89,186). Other immune mediators, such as tryptase, prostaglandins, and soluble ADAM8, have been detected to be elevated in the BAL fluid of CEP patients as well (7,109,132). BAL fluid eosinophils in CEP are activated, with expression of HLA-DR, and have longer survival than their counterparts in the peripheral blood, with decreased apoptosis (11,159).…”

Section: Cepmentioning

confidence: 94%

Eosinophilic Pneumonias

2012

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SUMMARY This review starts with discussions of several infectious causes of eosinophilic pneumonia, which are almost exclusively parasitic in nature. Pulmonary infections due specifically to Ascaris , hookworms, Strongyloides , Paragonimus , filariasis, and Toxocara are considered in detail. The discussion then moves to noninfectious causes of eosinophilic pulmonary infiltration, including allergic sensitization to Aspergillus , acute and chronic eosinophilic pneumonias, Churg-Strauss syndrome, hypereosinophilic syndromes, and pulmonary eosinophilia due to exposure to specific medications or toxins.

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Section: Cepmentioning

confidence: 94%

Eosinophilic Pneumonias

2012

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“…Bronchoalveolar lavage was performed as previously described [27,28]. Samples were obtained by instillation of four aliquots (about 60 ml each) of saline solution through a fiberoptic bronchoscope (Olympus IT-10).…”

Section: Bronchoalveolar Lavagementioning

confidence: 99%

Calgranulin B (S100A9/MRP14): A Key Molecule in Idiopathic Pulmonary Fibrosis?

et al. 2010

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Calgranulin B is a small calcium-binding protein with several immunological functions mainly involved in chronic inflammation and cancer. It can participate in recruitment of neutrophils and leukocytes in inflamed tissue, oxidant/antioxidant balance, adhesion of neutrophils to fibronectin, and regulation of apoptosis. In a previous proteomic study, we found that calgranulin B was up-regulated in the bronchoalveolar lavage (BAL) of patients with idiopathic pulmonary fibrosis (IPF) with respect to controls and patients with other interstitial lung diseases. The aims of this study are to compare calgranulin B concentrations in BAL of patients with IPF and sarcoidosis and controls by a quantitative method, to look for correlations with clinical data, and to evaluate calgranulin B expression in lung tissue of IPF patients by immunohistochemistry. A modification of a commercial ELISA was used to determine calgranulin B concentrations in BAL of 16 patients with IPF (a group of patients in which we previously performed proteomic analysis), 17 patients with sarcoidosis, and 7 controls. The immunohistochemistry was done in a subgroup of patients with IPF and a control group of lung transplant donors. Calgranulin B concentrations were significantly higher in patients with IPF than controls (p < 0.01); they were inversely correlated with FVC and DLCO values and directly correlated with neutrophil and eosinophil percentages in BAL. Immunohistochemistry revealed a patchy distribution of calgranulin B, predominantly around areas of fibrotic remodeling. Calgranulin B may be a trigger molecule involved in the evolution and progression of IPF, being overexpressed in BAL of patients with IPF with severe functional deterioration and in the peribronchiolar area bordering zones of honeycombing.

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“…It has immunological functions, regulating expression of several chemokines and cytokines [16]. Elevated concentrations of tryptase have been found in different pathological conditions: in serum of patients with acute anaphylaxis and mastocytosis [17], in nasal lavage fluid of allergic patients, in induced sputum and BAL of patients with asthma [18] and in 2005 we found increased tryptase levels in BAL of patients with chronic eosinophilic pneumonia [19]. Increased tryptase levels in BAL of patients with different ILD including sarcoidosis and IPF were first reported by Walls et al [20] and confirmed by Eklund who postulated involvement of tryptase in lung fibrotic damage [20,21].…”

Section: Introductionmentioning

confidence: 91%

“…Bronchosopy and bronchoalveolar lavage were performed at the time of diagnosis with the written informed consent of the patients as previously described [19]. BAL was obtained by instillation of four 60 ml aliquots of saline solution by fiberoptic bronchoscope (Olympus IT-10).…”

Section: Bronchoalveolar Lavage and Lymphocyte Phenotype Analysismentioning

confidence: 99%

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The Analysis of Tryptase in Serum of Sarcoidosis Patients

et al. 2009

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Sarcoidosis is a systemic granulomatous disease of unknown etiology characterized by activation of macrophages and T lymphocytes. Relatively little is known about the role of mast cells and their mediators in the pathogenesis of sarcoidosis. Tryptase is an enzyme produced by activated mast cells, regarded as a marker of mast cell activation. To analyse tryptase concentrations in serum of sarcoidosis patients in an attempt to define the role of tryptase and mast cells in the pathogenesis of sarcoidosis and to evaluate the potential of tryptase as marker of disease severity. Quantitative analysis of tryptase concentrations was performed in serum of patients with stable sarcoidosis (n = 12), progressive sarcoidosis (n = 23) and controls (n = 13). Patients enrolled in the study had been monitored at Siena Regional Referral Centre for Sarcoidosis from onset for at least 12 months. Significantly higher concentrations of tryptase were found in peripheral blood of sarcoidosis patients (6.08 +/- 3.98 microg/l) than controls (2.96 +/- 1.75microg/l; p = 0.012). Patients with progressive disease showed the highest tryptase concentrations in serum. Tryptase and mast cells may be involved in the immunopathogenesis of sarcoidosis and further studies are required to understand if tryptase may represent a marker of sarcoidosis severity.

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Tryptase concentrations in bronchoalveolar lavage from patients with chronic eosinophilic pneumonia

Cited by 17 publications

References 19 publications

Eosinophilic Pneumonias

Eosinophilic Pneumonias

Calgranulin B (S100A9/MRP14): A Key Molecule in Idiopathic Pulmonary Fibrosis?

The Analysis of Tryptase in Serum of Sarcoidosis Patients

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