Tryptophan degradation enzymes and Angiotensin (1−7) expression in human placenta

Silvano, Angela; Seravalli, Viola; Strambi, Noemi; Tartarotti, Enrico; Tofani, Lorenzo; Calosi, Laura; Parenti, Astrid; Tommaso, Mariarosaria Di

doi:10.1016/j.jri.2022.103692

Cited by 4 publications

(7 citation statements)

References 55 publications

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“…No difference in IDO1 mRNA expression was found between samples from vaginal delivery and cesarean section on either the maternal or fetal side of the placenta. TDO2 was higher in the maternal side compared to the fetal one after cesarean section, although the difference was not statistically significant (p = 0.06), as we also previously observed in placentas from vaginal delivery (Silvano et al, 2022). Interestingly, the mode of delivery seemed to affect TDO2 expression in the maternal side of placentas, as it was more expressed in samples from elective cesarean section than in those from vaginal delivery (p < 0.01).…”

Section: Resultssupporting

confidence: 47%

“…Fresh placental tissue and maternal blood samples were collected immediately after cesarean section. The control group consisted of 20 gestational agematched women with uncomplicated pregnancies who delivered vaginally, previously enrolled in another study (Silvano et al, 2022).…”

Section: Sample Collectionmentioning

confidence: 99%

“…Samples were obtained from the maternal surface (decidua basalis) and fetal surface (chorion and thin layer of villi) of placentas. One μg of total RNA was used for the reverse transcription reaction with Prime Script RT reagent Kit Takara (Otsu, Japan), the cDNAs were amplified with specific primers described below, as previously reported (Silvano et al, 2022). The SYBR Premix Ex Taq (Takara) was used for Quantitative Real-Time PCR (qRT-PCR), according to the instructions on a Rotorgene RG-3000A cycle system (Qiagen) platform.…”

Section: Real-time Pcr For Ido1 and Tdo2 Mrna Expressionmentioning

confidence: 99%

“…The SYBR Premix Ex Taq (Takara) was used for Quantitative Real-Time PCR (qRT-PCR), according to the instructions on a Rotorgene RG-3000A cycle system (Qiagen) platform. The 18 S rRNA housekeeping (Silvano et al, 2022) was used and the difference between CT values of the target genes and 18 S gene was used to calculate the delta CT. IDO1 primers were: IDO1 fw: 5'-AGTTCTGGGATGCAT-CACCA-3' and rev: 5'-CAGTTTCTTGGAGAGTTGGCA-3'. The cycle was set at 95 • C for 5 s, 55 • C or 52 • C for 30 s and 72 • C for 30 s, repeated 35 times.…”

Section: Real-time Pcr For Ido1 and Tdo2 Mrna Expressionmentioning

confidence: 99%

“…One study showed that IDO1 mRNA expression was significantly lower in the maternal side of placentas from pregnancies complicated by preeclampsia compared to healthy pregnancies delivered by cesarean section (Broekhuizen et al, 2020). Recently, TDO mRNA (TDO2) expression has also been studied in the placentas of uncomplicated term pregnancies delivered vaginally, showing that TDO2 was expressed both in the maternal and fetal side of the placenta (Silvano et al, 2022). However, until now, no study has compared IDO1 and TDO2 expression between the placentas of pregnancies delivered vaginally and those of pregnancies delivered by elective cesarean section in the absence of labor.…”

Section: Introductionmentioning

confidence: 99%

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Tryptophan degradation enzymes expression in the placenta and the Kynurenine/Tryptophan ratio in maternal plasma after elective cesarean section

Silvano

Seravalli

Strambi

et al. 2023

Journal of Reproductive Immunology

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Section: Resultssupporting

confidence: 47%

Section: Sample Collectionmentioning

confidence: 99%

Section: Real-time Pcr For Ido1 and Tdo2 Mrna Expressionmentioning

confidence: 99%

Section: Real-time Pcr For Ido1 and Tdo2 Mrna Expressionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Tryptophan degradation enzymes expression in the placenta and the Kynurenine/Tryptophan ratio in maternal plasma after elective cesarean section

Silvano

Seravalli

Strambi

et al. 2023

Journal of Reproductive Immunology

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Epigenetic programming of obesity in early life through modulation of the kynurenine pathway

Gharipour,

Craig,

Stephenson

2024

Int J Obes

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Childhood obesity is a global health concern that has its origins before birth. Although genetics plays a crucial role, increasing evidence suggests that epigenetic modifications during fetal life could also influence its incidence. In this model, during the fetal period, interactions between genetic makeup, intrauterine factors, and environmental conditions, increase the risk of childhood obesity. This is in accordance with the Developmental Origins of Health and Disease (DOHaD) hypothesis, in which specific intrauterine environments can have long-lasting effects on the immune system’s essential functions during crucial stages of fetal growth, resulting in permanent changes to the immune function of the offspring. Consequently, dysfunction can consequently make the offspring more prone to inflammatory and immune-related disorders later in life. In this review, we examine how maternal inflammation could influence the risk of childhood obesity. We propose that during pregnancy, modification of the expression of critical genes in metabolic and signaling pathways, such as the kynurenine (Kyn) pathway, occurs due to increased levels of maternal inflammation. We also propose that such expression differences are mediated by epigenetic changes. Furthermore, we also hypothesize that the Kyn pathway produces metabolites that have immunoregulatory effects and may play a crucial role in regulating inflammation during pregnancy. As a result, interventions aimed at improving maternal inflammation may be able to help alleviate the risk of childhood obesity.

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G-protein-coupled receptor MAS deletion produces a preeclampsia-like phenotype in FVB/N mice

Vieira Pimentel,

Braga,

Velloso

et al. 2023

Clinical Science

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Background - An unbalance in the renin-angiotensin (Ang) system between the Ang II/AT1 and Ang-(1-7)/Mas axis appears to be involved in preeclampsia (PE), in which a reduction of Ang-(1-7) was observed. Here we tested whether the reduction in the activity of the Ang-(1-7)/Mas axis could be a contributing factor for the development of PE, using Mas-deficient (Mas-/-) mice. Methods and Results - Cardiovascular parameters were evaluated by telemetry before, during pregnancy and 4 days post partum in twenty weeks old Mas-/- and wildtype (WT) female mice. Mas-/- mice presented reduced arterial blood pressure at baseline (91.3 ± 0.8 in Mas-/- vs. 94.0 ± 0.9 mmHg in WT, Diastolic, p<0.05). However, after the 13th day of gestation, blood pressure in Mas-/- mice started to increase, time-dependently, and at day 19 of pregnancy, these animals presented a higher blood pressure in comparison to WT group (90.5 ± 0.7 in Mas-/- vs. 80.3 ± 3.5 mmHg in WT, Diastolic D19, p<0.0001). Moreover, pregnant Mas-/- mice presented fetal growth restriction, increase in urinary protein excretion as compared to non-pregnant Mas-/- , oliguria, increase in cytokines, endothelial dysfunction and reduced ACE, AT1R, ACE2, ET-1A and eNOS placental mRNA, similar to some of the clinical manifestations found in the development of preeclampsia. Conclusions- These results show that Mas-deletion produces a preeclampsia-like state in FVB/N mice. Key Words: Mas-deficient mice - preeclampsia - Angiotensin-(1-7) / Mas axis

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Tryptophan degradation enzymes and Angiotensin (1−7) expression in human placenta

Cited by 4 publications

References 55 publications

Tryptophan degradation enzymes expression in the placenta and the Kynurenine/Tryptophan ratio in maternal plasma after elective cesarean section

Tryptophan degradation enzymes expression in the placenta and the Kynurenine/Tryptophan ratio in maternal plasma after elective cesarean section

Epigenetic programming of obesity in early life through modulation of the kynurenine pathway

G-protein-coupled receptor MAS deletion produces a preeclampsia-like phenotype in FVB/N mice

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