Tryptophan tags and de novo designed complementary affinity ligands for the expression and purification of recombinant proteins

Pina, Ana Sofia; Carvalho, Sara; Dias, Ana; Guilherme, Márcia; Caraça, Luciana T.; Coroadinha, Ana S.; Lowe, Christopher R.; Roque, Ana C. A.

doi:10.1016/j.chroma.2016.10.017

Cited by 10 publications

(6 citation statements)

References 26 publications

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“…In the years that thereafter, this Ugi-modification approach opens a new window to the combinatorial synthesis of pseudobiospecific ligands modified sepharose-supports for purification of diverse kinds of proteins, caused to several reports based on the same methodology . The synthetic ligands have been employed efficiently for mimic Protein G for the purification of mammalian immunoglobulins 72 , mimicking the third domain of Protein G (domain III) and isolate highly pure IgG and Fab fragments from mammalian and yeast cell extracts 73 , purification of recombinant human erythropoietin (rHuEPO) 74 , interacting with glycan moieties on glycoproteins 75 , purification of green fluorescent protein (GFP) or GFP fusion proteins 76 , and expression and purification of fusion proteins through “tag–receptor” affinity pair 77 . (Scheme )…”

Section: Covalent Functionalization Through Multicomponent Reactionsmentioning

confidence: 99%

Materials Functionalization with Multicomponent Reactions: State of the Art

Afshari

Shaabani

2018

ACS Comb. Sci.

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The emergence of neoteric synthetic routes for materials functionalization is an interesting phenomenon in materials chemistry. In particular, the union of materials chemistry with multicomponent reactions (MCRs) opens a new avenue leading to the realm of highly innovative functionalized architectures with unique features. MCRs have recently been recognized as considerable part of the synthetic chemist's toolbox due to their great efficiency, inherent molecular diversity, atom and pot economy along with operational simplicity. Also, MCRs can improve E-factor and mass intensity as important green chemistry metrics. By rational tuning of the materials, as well as the MCRs, wide ranges of functionalized materials can be produced with tailorable properties that can play important roles in the plethora of applications. To date, there has not reported any exclusive review of a materials functionalization with MCRs. This critical review highlights the state-of-the-art on the one-pot functionalization of carbonaceous and siliceous materials, polysaccharides, proteins, enzymes, synthetic polymers, etc., via diverse kind of MCRs like Ugi, Passerini, Petasis, Khabachnik-Fields, Biginelli, and MALI reactions through covalent or noncovalent manners. Besides the complementary discussion of synthetic routes, superior properties and detailed applicability of each functionalized material in modern technologies are discussed. Our outlook also emphasizes future strategies for this unprecedented area and their use as materials for industrial implementation. With no doubt, MCRs-functionalization of materials bridges the gap between materials science domain and applied chemistry.

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Section: Covalent Functionalization Through Multicomponent Reactionsmentioning

confidence: 99%

Materials Functionalization with Multicomponent Reactions: State of the Art

Afshari

Shaabani

2018

ACS Comb. Sci.

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“…Recently, new strategies have been developed to purify arginine-rich peptides, namely the Argi system based on the AR aptamer selected to capture Arg-based tags (K D ~ 10 −7 M) ( Table 1 and Table 2 ) [48] . The properties of several tryptophan (Trp)-based tags have also been screened for single-step purification through the development of complementary affinity ligands [49] . Pina et al [49] demonstrated that two new hexapeptide tag variants ( Table 1 ) – (NW)3 and (WF)3 – fulfill the characteristics needed for a good affinity tag and showed that their system based in “tag-receptor” affinity pairs (K D ~ 10 −7 M) works similarly to other commonly used affinity-based purification systems.…”

Section: Protein Purification/immobilization Tagsmentioning

confidence: 99%

“…The properties of several tryptophan (Trp)-based tags have also been screened for single-step purification through the development of complementary affinity ligands [49] . Pina et al [49] demonstrated that two new hexapeptide tag variants ( Table 1 ) – (NW)3 and (WF)3 – fulfill the characteristics needed for a good affinity tag and showed that their system based in “tag-receptor” affinity pairs (K D ~ 10 −7 M) works similarly to other commonly used affinity-based purification systems. Therefore, these pairs can be potentially used for protein immobilization as well.…”

Section: Protein Purification/immobilization Tagsmentioning

confidence: 99%

Tag-mediated single-step purification and immobilization of recombinant proteins toward protein-engineered advanced materials

Freitas

Domingues

Aguiar

2022

Journal of Advanced Research

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“…C-C and C-N bond-forming U-4CR can constitute a scaffold providing enormous molecular diversity. U-4CR protocol has been utilized in various fields of chemistry including separation science where it has been employed mainly in affinity chromatography [26][27][28][29][30][31][32][33][34][35][36][37][38][39]. Surprisingly, up to the present time, its adoption in other chromatographic modes has been rather scarce.…”

Section: Introductionmentioning

confidence: 99%

New multimodal stationary phases prepared by Ugi multicomponent approach

Sýkora

Záruba

Butnariu

et al. 2020

J of Separation Science

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Eight different stationary phases based on two aminopropyl silicas of different brands suitable for multimodal chromatography applications have been prepared by a four-component Ugi reaction. The intention was to synthesize stationary phases significantly differing in their properties hereby demonstrating flexibility of the Ugi synthetic protocol. Diverse functional groups including a nonpolar long aliphatic chain, phenyl moiety, cholic acid scaffold, phenylboronic and monosaccharide units, charged betaine, and arginine moieties were immobilized on a silica surface. The novel sorbents were extensively characterized by elemental analysis, Raman spectroscopy, and chromatography. Considering the anchored chemical structures covalently bonded to the silica surface, reversed-phase, hydrophilic, and ion-exchange separation modes were expected. The chromatographic evaluation was performed directed to map the potential of the individual columns specifically in the mentioned chromatographic modes. The Ugi synthetic protocol has proven to be a simple, feasible, and versatile tool for the synthesis of sorbents of variable properties. The newly prepared stationary phases differed considerably in hydrophobicity and ion-exchange ability. A significant influence of the supporting aminopropyl silica on the final chromatographic behavior was observed. Finally, one practical example confirming applicability of the newly prepared sorbents was demonstrated in separation of cytarabine.

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Tryptophan tags and de novo designed complementary affinity ligands for the expression and purification of recombinant proteins

Cited by 10 publications

References 26 publications

Materials Functionalization with Multicomponent Reactions: State of the Art

Materials Functionalization with Multicomponent Reactions: State of the Art

Tag-mediated single-step purification and immobilization of recombinant proteins toward protein-engineered advanced materials

New multimodal stationary phases prepared by Ugi multicomponent approach

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