Tsetse and trypanosomosis in Africa : the challenges, the opportunities : vector-borne diseases : trypanosomosis

Ilemobade, A. A.

doi:10.4102/ojvr.v76i1.59

Cited by 42 publications

(37 citation statements)

References 3 publications

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“…African trypanosomiases are neglected tropical diseases that perpetuate poverty through their burden on both public health and agriculture [1]. Human African trypanosomiasis (HAT) or sleeping sickness occurs in remote sub-Saharan areas and is caused by two human infective subspecies of the protozoan parasite Trypanosoma brucei (T.b.)…”

Section: Introductionmentioning

confidence: 99%

Recombinant Antigens Expressed in Pichia pastoris for the Diagnosis of Sleeping Sickness Caused by Trypanosoma brucei gambiense

et al. 2014

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BackgroundScreening tests for gambiense sleeping sickness, such as the CATT/T. b. gambiense and a recently developed lateral flow tests, are hitherto based on native variant surface glycoproteins (VSGs), namely LiTat 1.3 and LiTat 1.5, purified from highly virulent trypanosome strains grown in rodents.Methodology/Principal FindingsWe have expressed SUMO (small ubiquitin-like modifier) fusion proteins of the immunogenic N-terminal part of these antigens in the yeast Pichia pastoris. The secreted recombinant proteins were affinity purified with yields up to 10 mg per liter cell culture.Conclusions/SignificanceThe diagnostic potential of each separate antigen and a mixture of both antigens was confirmed in ELISA on sera from 88 HAT patients and 74 endemic non-HAT controls. Replacement of native antigens in the screening tests for sleeping sickness by recombinant proteins will eliminate both the infection risk for the laboratory staff during antigen production and the need for laboratory animals. Upscaling production of recombinant antigens, e.g. in biofermentors, is straightforward thus leading to improved standardisation of antigen production and reduced production costs, which on their turn will increase the availability and affordability of the diagnostic tests needed for the elimination of gambiense HAT.

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Section: Introductionmentioning

confidence: 99%

Recombinant Antigens Expressed in Pichia pastoris for the Diagnosis of Sleeping Sickness Caused by Trypanosoma brucei gambiense

et al. 2014

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“…It influences both public health and agricultural development (Ilemobade 2009). It remains one of the biggest infectious disease constraints to livestock production in the region where estimates of 5-10% increase in GDP and almost $5 billion dollars savings (from annual losses) are projected once the disease is controlled (Auty et al, 2015;Ilemobade 2009).…”

Section: Introductionmentioning

confidence: 99%

Trypanosomiaisis in Non Migratory Cattle in Suburban Kaduna

Dauda¹,

Abubakar²,

Muhammad³

et al. 2017

GJAS

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African trypanosomiasis is a disease complex, prevalent in sub-Saharan Africa, influencing both public health and agricultural development. The disease is also becoming increasingly prevalent beyond its traditionally defined realm. Studies have shown that migration increases the risk of cattle being infected with trypanosomes and that Trypanosoma brucei populations in cattle (and other domestic animals) could serve as reservoirs for the human infection. This study involves an investigation into the prevalence of Trypanosomiasis among cattle herds kept extensively but do not seasonally migrate. It was conducted in Guguwa-Rigasa, Igabi local government area of Kaduna State, North-West Nigeria.A total of 157 cattle, sheep, goats and dogs were sampled from three herds.Parasitological examination was carried out using the Standard Trypanosome Detection Method. Trypanosome species were identified based on their motility using the Buffy coat method and morphological features from Giemsa stained films. A total of 25 (15.92%) animals were found to be positive with Trypanosoma congolense and T. brucei. It can be concluded that animal trypanosomiasis is prevalent in the area. However, further and more sensitive studies need to be carried out to bring more clarity on the parasite species, vectors and overall epidemiology of the disease.

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“…Trypanosomiasis, also known as surra, nagana, Peste-boba, Tahaga, Kaodzera, Baleri, Gambian horse sickness, Cachexial fevers, Dourine, mal de las caderas, etc., is a disease in cattle, buffaloes, sheep, goats, deer, camelids, antelopes, lamas, donkeys, horses, mules, deer, llamas, pigs, and elephants caused by a several flagellated protozoan species of the genus Trypanosoma: Trypanosoma evansi, Trypanosoma equinum, Trypanosoma brucei, Trypanosoma vivax, and Trypanosoma congolense (FAO-WHO, 1969;Takken et al, 1988;Dargie et al, 1993;Cecchi et al, 2009;Ilemobade, 2009;Health, 2012). It is transmitted from animal to animal mechanically by hematophagous flies, including Tabanus spp., Musca spp., Lyperosia spp., Stomoxys, and Atylotus genera.…”

Section: Trypanosomiasismentioning

confidence: 99%

Animal Health: Ectoparasites

Maggi

2014

Encyclopedia of Agriculture and Food Systems

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Tsetse and trypanosomosis in Africa : the challenges, the opportunities : vector-borne diseases : trypanosomosis

Cited by 42 publications

References 3 publications

Recombinant Antigens Expressed in Pichia pastoris for the Diagnosis of Sleeping Sickness Caused by Trypanosoma brucei gambiense

Recombinant Antigens Expressed in Pichia pastoris for the Diagnosis of Sleeping Sickness Caused by Trypanosoma brucei gambiense

Trypanosomiaisis in Non Migratory Cattle in Suburban Kaduna

Animal Health: Ectoparasites

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