Tsga10 Encodes a 65-Kilodalton Protein That Is Processed to the 27-Kilodalton Fibrous Sheath Protein1

Modarressi, Mohammad Hossein; Behnam, Babak; Cheng, Min-Chih; Taylor, Kmg; Wolfe, Jonathan; Hoorn, Frans A. van der

doi:10.1095/biolreprod.103.021170

Cited by 50 publications

(46 citation statements)

References 36 publications

(49 reference statements)

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“…The gene is located on chromosome 2q11.2 and consists of 19 exons extending over 3 kb. TSGA10 is expressed during spermatogenesis and codes for a protein found in the fibrous sheath, a major sperm tail structure in mature spermatozoa in the mouse (19). In this study, we showed that the testis-specific gene TSGA10 is over-expressed in some types of malignancies and induces an IgG response in patients.…”

Section: Discussionmentioning

confidence: 63%

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Over‐Expression of the Testis‐Specific Gene TSGA10 in Cancers and Its Immunogenicity

Tanaka

Ono

Sato

et al. 2004

Microbiology and Immunology

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Human tumor antigens recognized by an autologous host have been identified, and the list of antigens recognized by CD8 T cells (9,25,36,37), CD4 T cells (3,10,24,39), and antibodies (8,21,26) is rapidly growing. They can be categorized into the following groups: differentiation antigens (2,5,14), mutational antigens (27), amplified/over-expressed antigens (23), splice variant antigens (26, 28), viral antigens (16, 33), and cancer/testis (CT) antigens (4, 26). CT antigens have received particular attention because of their unique expression pattern and potential as targets for cancer vaccines (29,41). The characteristics of CT antigens include high levels of expression in male germ cells, lack of expression in other normal tissues, and aberrant expression in a wide range of tumors. There are now more than 20 genes or gene families known to code for CT antigens (29, 41).The identification of CT antigens has been primarily based on the detection of the candidate antigen/gene in tumors followed by their characterization using a panel of normal tissues and various tumors. The testis-specific expression of CT antigens may prompt one to investigate whether previously defined sperm proteins are also expressed in tumors. We have been studying the expression in tumors of proacrosin binding protein sp32 precursor present in spermatozoa (22) and A-kinase anchoring protein 3 (AKAP3) present in the sperm head and flagellum (7). Real-time RT-PCR analysis using a TaqMan probe showed mRNA over-expression associated with those sperm proteins in some tumors compared to corresponding normal tissues. In this study, we investigated TSGA10 mRNA expression in normal tissues and tumors. TSGA10 was originally identified by differential mRNA display as a testis-specific gene (18,19). Quantitative real-time RT-PCR analysis showed over-expres- Editor-Communicated Paper Over-Expression of the Testis-Specific Gene

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Section: Discussionmentioning

confidence: 63%

“…In this study, we investigated TSGA10 mRNA expression in normal tissues and tumors. TSGA10 was originally identified by differential mRNA display as a testis-specific gene (18,19). Quantitative real-time RT-PCR analysis showed over-expres- …”

mentioning

confidence: 99%

Over‐Expression of the Testis‐Specific Gene TSGA10 in Cancers and Its Immunogenicity

Tanaka

Ono

Sato

et al. 2004

Microbiology and Immunology

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“…These controls have previously been employed in the successful optimisation of other cell-based assays used for this purpose. Firstly, transfection efficiency was checked using the well validated method of GFP transfection to produce auto-fluorescent cells (Modarressi et al 2004). This confirmed our method of transfection was robust.…”

Section: Study Limitationsmentioning

confidence: 55%

The role of humoral autoimmunity in gastrointestinal neuromuscular diseases

Hubball

Martin

Lang

et al. 2009

Progress in Neurobiology

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Dysfunction of the gastrointestinal neuromuscular apparatus (including interstitial cells ofCajal) is presumed to underlie a heterogeneous group of disorders collectively termed gastrointestinal neuromuscular diseases (GINMDs). Humoral (antibody)-mediated autoimmunity directed against ligand-or voltage-gated ion channels or associated proteins involved in neuromuscular transmission is associated with several acquired neuromuscular diseases of the periphery and is now implicated in an increasing number of less well-characterised diseases. Anti-channel antibodies have been reported in small numbers of patients with GINMD, particularly in those with GI dysfunction secondary to an underlying disease such as neoplasia or infection. However, little is known of humoral autoimmunity in primary GINMDs.This thesis investigated the association between anti-channel antibodies and GINMD, and the human GI tract as a potential target of anti-channel antibodies. The presence of antivoltage-and ligand-gated antibodies was investigated in the serum of patients with primary achalasia, enteric dysmotility and intestinal pseudo-obstruction, and in those with GINMD secondary to Chagas' disease. The functional effect of sera from some of these patients on colonic smooth muscle contractility was also characterised. Finally, the distribution of six voltage-gated potassium channels (VGKCs), which serve essential roles in the peripheral and central nervous systems and are known targets of pathological autoantibodies, were investigated in all layers of the human GI tract.Our findings suggest that currently recognised anti-channel antibodies are not a common pathogenic factor in primary GINMDs. Anti-channel antibodies, in particular anti-VGKC antibodies, were however found in a significant number of individuals with Chagas' disease. Furthermore, circulating factors in patients with chagasic GI disease altered GI smooth muscle contractility in vitro which may have pathological relevance to GI

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“…Mouse mHIF-1aI.1 and human hHIF1aTe are expressed in the postacrosomal region and the midpiece of spermatozoa [16,17]. Here, we demonstrate that HIF-1a interacts with the filament-building sperm protein TSGA10 [18], which modulates the intracellular localization of HIF-1a. Since TSGA10 expression was recently demonstrated also in embryonic tissue, different adult organs like brain, kidney and heart as well as malignant tissues, this finding may have also implications for tissue-dependent regulation of HIF-1 activity [19].…”

Section: Introductionmentioning

confidence: 67%

“…Fulllength TSGA10 (Accession No. AF530050) was amplified by PCR from pEGFP2mTSGA10 [18]. Fragments of TSGA10 corresponding to the indicated amino acids were amplified by PCR from the fulllength entry vector.…”

Section: Plasmid Constructionsmentioning

confidence: 99%

TSGA10 prevents nuclear localization of the hypoxia‐inducible factor (HIF)‐1α

et al. 2006

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The hypoxia-inducible factor (HIF)-1 is a transcriptional regulator of genes involved in oxygen homeostasis. We previously described testis-specific isoforms of HIF-1a (mHIF1aI.1 and hHIF-1aTe). Using mHIF-1a exon I.1 knock-out mice we confirmed the specific expression of mHIF-1aI.1 in the sperm tail. A protein-protein interaction between HIF-1a and the testis specific gene antigen 10 (TSGA10) was identified by yeast twohybrid screening. TSGA10 is expressed in testis but also in other organs and malignant tissues. Immunofluorescence analysis indicated that the C-terminal part of TSGA10 accumulates in the midpiece of spermatozoa, where it co-localizes with HIF-1a. HIF-1a nuclear localization and HIF-1 transcriptional activity were significantly affected by overexpressed TSGA10.

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Tsga10 Encodes a 65-Kilodalton Protein That Is Processed to the 27-Kilodalton Fibrous Sheath Protein1

Cited by 50 publications

References 36 publications

Over‐Expression of the Testis‐Specific Gene TSGA10 in Cancers and Its Immunogenicity

Over‐Expression of the Testis‐Specific Gene TSGA10 in Cancers and Its Immunogenicity

The role of humoral autoimmunity in gastrointestinal neuromuscular diseases

TSGA10 prevents nuclear localization of the hypoxia‐inducible factor (HIF)‐1α

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