TSGA10 overexpression inhibits angiogenesis of HUVECs: A HIF-2α biased perspective

Amoorahim, Mahtab; Valipour, Elahe; Hoseinkhani, Zohreh; Mahnam, Azadeh; Rezazadeh, Davood; Ansari, Mohabbat; Shahlaei, Mohsen; Gamizgy, Younes Hossainy; Moradi, Sajad; Mansouri, Kamran

doi:10.1016/j.mvr.2019.103952

Cited by 9 publications

(6 citation statements)

References 47 publications

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“…The present study is in agreement with those recognizing TSGA10 as a tumor suppressor gene (3). Our previous study illustrated that TSGA10 overexpression could inhibit angiogenesis and tumor cell proliferation, migration and invasion by inhibiting HIF-1α (53). We demonstrated that in order to exerting antitumor effects of TSGA10 protein on cancer cells, its expression should reach a certain threshold.…”

Section: Defective Function Ofmentioning

confidence: 91%

TSGA10, a new player in regulating autophagy and apoptosis

Rezazadeh

Ranjbarnejad

Mansouri

et al. 2022

Preprint

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Testis specific gene antigen 10 (TSGA10) overexpression could inhibit angiogenesis and tumor cell proliferation, migration and invasion through inhibiting HIF-1α. The exact role of TSGA10 in autophagy and apoptosis is not clear. Present study was conducted to investigate the potential effects of TSGA10 overexpression on regulation of autophagy and apoptosis. To do so, TSGA10-containing vector (pcDNA3.1-TSGA10 vector) was designed for stable and transient transfections in HeLa cells, and clonal selection was applied. Expression of autophagy and apoptosis-related genes was assessed by real-time RT-PCR in TSGA10-overexpressing cells compared with HeLa cells transfected by empty vector. Rate of autophagy and cell viability were assessed by acridine orange and MTT assays, respectively. Our findings showed that overexpression of TSGA10 would induce autophagy under normoxic and hypoxic conditions in the presence or absence of autophagy inducers. Expression levels of autophagy and apoptosis-related genes increased significantly in TSGA10-overexpressing cells compared with control cells. In addition, HeLa cells overexpressing TSGA-10 exhibited lower expression of BCL-2 and survival rates in comparison with control cells. Molecular docking results showed that interaction between TSGA10 and BCL-2 could inhibit anti-apoptotic activity of BCL-2. According to our findings, TSGA10 overexpression has a main role in regulating autophagy and apoptosis through inhibition of BCL-2. Therefore, TSGA10 could be considered as a new regulator of autophagy and apoptosis in future studies.

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Section: Defective Function Ofmentioning

confidence: 91%

TSGA10, a new player in regulating autophagy and apoptosis

Rezazadeh

Ranjbarnejad

Mansouri

et al. 2022

Preprint

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“…One of them is TSGA10. It has been shown that the over expression of the compound is associated with decreased HIF-1α transcriptional activity as well as disruption of HIF-1α axis ( Mansouri et al, 2016 ; Amoorahim et al, 2020 ).…”

Section: Potential Biological Mechanisms and Function Of Nps Against ...mentioning

confidence: 99%

Natural Products from Actinobacteria as a Potential Source of New Therapies Against Colorectal Cancer: A Review

et al. 2022

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Colorectal cancer (CRC) is a common, and deadly disease. Despite the improved knowledge on CRC heterogeneity and advances in the medical sciences, there is still an urgent need to cope with the challenges and side effects of common treatments for the disease. Natural products (NPs) have always been of interest for the development of new medicines. Actinobacteria are known to be prolific producers of a wide range of bioactive NPs, and scientific evidence highlights their important protective role against CRC. This review is a holistic picture on actinobacter-derived cytotoxic compounds against CRC that provides a good perspective for drug development and design in near future. This review also describes the chemical structure of 232 NPs presenting anti-CRC activity with the being majority of quinones, lactones, alkaloids, peptides, and glycosides. The study reveals that most of these NPs are derived from marine actinobacteria followed by terrestrial and endophytic actinobacteria, respectively. They are predominantly produced by Streptomyces, Micromonospors, Saliniospors and Actinomadura, respectively, in which Streptomyces, as the predominant contributor generating over 76% of compounds exclusively. Besides it provides a valuable snapshot of the chemical structure-activity relationship of compounds, highlighting the presence or absence of some specific atoms and chemical units in the structure of compounds can greatly influence their biological activities. To the best of our knowledge, this is the first comprehensive review on natural actinobacterial compounds affecting different types of CRC. Our study reveals that the high diversity of actinobacterial strains and their NPs derivatives, described here provides a new perspective and direction for the production of new anti-CRC drugs and paves the way to innovation for drugs discovery in the future. The knowledge obtain from this review can help us to understand the pivotal application of actinobacteria in future drugs development.

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“…CTAs have divergent functions in the regulation of tumorigenesis. Apart from the oncogenic CTAs mentioned above, several other CTAs have recently been reported as tumour suppressors that can inhibit cancer cell proliferation, angiogenesis and metastasis ( 71 ). For example, testis-specific gene antigen 10 (TSGA10) suppresses cancer development in various types of malignant tumours by inhibiting HIF-1 expression, tumour cell metastatic capability, and metabolic activity in breast cancer ( 72 ).…”

Section: The Role Of Cancer Testis Antigens In Tumorigenesis and Meta...mentioning

confidence: 99%

Cancer/Testis Antigens as Biomarker and Target for the Diagnosis, Prognosis, and Therapy of Lung Cancer

et al. 2022

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Lung cancer is the leading type of malignant tumour among cancer-caused death worldwide, and the 5-year survival rate of lung cancer patients is only 18%. Various oncogenes are abnormally overexpressed in lung cancer, including cancer/testis antigens (CTAs), which are restrictively expressed in the male testis but are hardly expressed in other normal tissues, if at all. CTAs are aberrantly overexpressed in various types of cancer, with more than 60 CTAs abnormally overexpressed in lung cancer. Overexpression of oncogenic CTAs drives the initiation, metastasis and progression of lung cancer, and is closely associated with poor prognosis in cancer patients. Several CTAs, such as XAGE, SPAG9 and AKAP4, have been considered as biomarkers for the diagnosis and prognostic prediction of lung cancer. More interestingly, due to the high immunogenicity and specificity of CTAs in cancer, several CTAs, including CT45, BCAP31 and ACTL8, have been targeted for developing novel therapeutics against cancer. CTA-based vaccines, chimeric antigen receptor-modified T cells (CAR-T) and small molecules have been used in lung cancer treatment in pre-clinical and early clinical trials, with encouraging results being obtained. However, there are still many hurdles to be overcome before these therapeutics can be routinely used in clinical lung cancer therapy. This review summarises the recent rapid progress in oncogenic CTAs, focusing on CTAs as biomarkers for lung cancer diagnosis and prognostic prediction, and as targets for novel anti-cancer drug discovery and lung cancer therapy. We also identify challenges and opportunities in CTA-based cancer diagnosis and treatment. Finally, we provide perspectives on the mechanisms of oncogenic CTAs in lung cancer development, and we also suggest CTAs as a new platform for lung cancer diagnosis, prognostic prediction, and novel anti-cancer drug discovery.

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TSGA10 overexpression inhibits angiogenesis of HUVECs: A HIF-2α biased perspective

Cited by 9 publications

References 47 publications

TSGA10, a new player in regulating autophagy and apoptosis

TSGA10, a new player in regulating autophagy and apoptosis

Natural Products from Actinobacteria as a Potential Source of New Therapies Against Colorectal Cancer: A Review

Cancer/Testis Antigens as Biomarker and Target for the Diagnosis, Prognosis, and Therapy of Lung Cancer

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