Tsukamurella inchonensis sp. nov.

Yassin, A. F.; Rainey, Fred A.; Brzezinka, H.; Burghardt, Jutta; Lee, H. J.; Schaal, K. P.

doi:10.1099/00207713-45-3-522

Cited by 92 publications

(50 citation statements)

References 34 publications

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“…These results suggest that strain IMMIB D-1488 T and IMMIB R-222 belong to a genetically distinct Corynebacterium species showing close relatedness with Corynebacterium minutissimum (98n8 % sequence similarity). It has been demonstrated previously, that high levels of 16S rRNA gene sequence similarity between strains belonging to the same genus do not indicate membership of the same species (Yassin et al, 1995(Yassin et al, , 1996(Yassin et al, , 2000. Here again it is clear that high 16S rRNA sequence similarities can be found between strains that have low DNA-DNA reassociation values since strains IMMIB D-1488 T and IMMIB R-222 share 98n8 % gene sequence similarity but only 42 % reassociation with the type strain of Corynebacterium minutissimum.…”

supporting

confidence: 52%

Corynebacterium aurimucosum sp. nov. and emended description of Corynebacterium minutissimum Collins and Jones (1983).

Yassin¹,

Steiner²,

Ludwig³

2002

International Journal of Systematic and Evolutionary Microbiology

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supporting

confidence: 52%

Corynebacterium aurimucosum sp. nov. and emended description of Corynebacterium minutissimum Collins and Jones (1983).

Yassin¹,

Steiner²,

Ludwig³

2002

International Journal of Systematic and Evolutionary Microbiology

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“…Nevertheless, in our case, the isolate could be differentiated from T. inchonensis because our species does not grow at 45°C and from T. strandjordii on the basis of its sugar utilization profile, since T. strandjordii uses inulin, arbutin, and D-melezitose as carbon sources, whereas our isolate does not (14,15).…”

mentioning

confidence: 90%

Tsukamurella pulmonis Bloodstream Infection Identified by secA1 Gene Sequencing

et al. 2015

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CASE REPORTA 3-year-old female was diagnosed with acute lymphoblastic leukemia (ALL), with intermediate risk (immunophenotype pre-B), when she was 23 months old. In order to start her chemotherapy, a central venous access device (subcutaneous port [SP]) was implanted in September 2009. The patient was treated with SHOP-05 (Spanish Pediatric Hematology Society-2005) protocols for high-risk ALL, and she was in complete remission after the induction phase and completed four consolidation and reinduction phases, as was mandatory. Near the end of the second year of maintenance, when she was receiving only oral mercaptopurine (50 mg/m 2 /daily) and intramuscular methotrexate (20 mg/m 2 / weekly), the patient had a fever peak 5 h after a blood collection from the SP. The patient was treated empirically with oral cefixime (8 mg/kg of body weight/daily) when the fever started because she had had a prior isolation documented as Klebsiella pneumoniae that was sensitive to cephalosporins, and since she was not neutropenic and had no other signs or symptoms of interest, outpatient management was tried first. We obtained one pediatric blood culture, which grew long, slightly curved, thin, nonbranching Gram-positive rods. Culture of blood samples after 48 h of incubation at 35°C Ϯ 2°C in 5% CO 2 on chocolate and blood agar showed small, rough, whitish colonies which did not produce aerial mycelium. The biochemical profile of the isolate obtained with the API Coryne identification system (bioMérieux, Marcy l'Etoile, France) after 48 h of incubation (2150004) corresponded to Rhodococcus spp. at a confidence level of 82.9%. By sequencing the 16S rRNA gene with the universal primers PA (5=-AGAGTTTGATCC TGGCTCAG-3=) and PLO6R (5=-GCGCTCGTTGCGGGACTTA ACC-3=) and the internal primer UP1R (5=-TTACCGCGGCTGC TGGCAC-3=), we obtained a sequence of 1,025 nucleotides that revealed maximal identity with Tsukamurella pulmonis (GenBank accession number AB564289). However, the 16S rRNA gene sequences of different Tsukamurella spp. were shown to be quite similar (99%).Two weeks later, due to the persistence of intermittent fever in spite of cefixime therapy and two subsequent blood culture sets again positive for the same bacterium (T. pulmonis), the patient was admitted to the hospital. Antibiotic susceptibility was determined after 48 to 72 h of incubation at 35°C Ϯ 2°C in ambient air by gradient diffusion (Etest; bioMérieux, Marcy l'Etoile, France) using Mueller-Hinton agar plates. Interpretations were based on CLSI breakpoints for mycobacteria, nocardiae, and other aerobic actinomycetes but not breakpoints for meropenem (MIC Ͼ 32 g/ml) and vancomycin (MIC, 2 g/ml) because they were not assessed in this document. The isolate was susceptible to amikacin (MIC, 1 g/ml), tobramycin (MIC, 4 g/ml), ciprofloxacin (MIC, 0.25 g/ml), clarithromycin (MIC, 0.125 g/ml), and linezolid (MIC, 1 g/ml) and resistant to amoxicillin-clavulanic acid (MIC Ͼ 256 g/ml) and ceftriaxone (MIC Ͼ 32 g/ml).For 3 days, she received ertapenem intravenously (15 mg/kg/12 h), ...

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“…Gram and ZiehlNeelsen stains were applied to hyphae grown on Columbia agar. Growth at different temperatures was determined at 10, 20, 27, 37 and 42 u C and a range of phenotypic characteristics was examined using standard procedures (Gordon, 1966(Gordon, , 1967Gordon & Mihm, 1957;Yassin et al, 1995). Tolerance to NaCl (0-16 %) was tested in GYM broth.…”

mentioning

confidence: 99%

Nocardiopsis potens sp. nov., isolated from household waste

Yassin

Spröer

Hupfer

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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The taxonomic position of an actinomycete, designated strain IMMIB L-21 T , was determined using a polyphasic taxonomic approach. The organism, which had phenotypic properties consistent with its classification in the genus Nocardiopsis, formed a distinct clade in the 16S rRNA gene sequence tree together with the type strain of Nocardiopsis composta, but was readily distinguished from this species using DNA-DNA relatedness and phenotypic data. The genotypic and phenotypic data show that the organism represents a novel species of the genus Nocardiopsis, for which the name Nocardiopsis potens sp. nov. is proposed. The type strain is IMMIB L-21 T (5DSM 45234 T 5CCUG 56587 T ).The genus Nocardiopsis was proposed by Meyer (1976) on the basis of chemotaxonomic and morphological characteristics. At the time of writing, the taxon encompasses 28 recognized species and 4 subspecies, which form a distinct clade within the evolutionary radiation occupied by members of the family Nocardiopsaceae (Rainey et al., 1996). Nocardiopsis strains are frequently isolated from saline soils (Yassin et al., 1993a;Li et al., , 2006, but they have also been recovered from an alkaline slag dump (Schippers et al., 2002), indoor environments (Peltola et al., 2001), the atmosphere of a composting facility (Kämpfer et al., 2002) and clinical material (Bernatchez & Lebreux, 1991;Yassin et al., 1997). In the present polyphasic study, an actinomycete isolated from household waste was shown to represent a novel species of the genus Nocardiopsis.Strain IMMIB L-21 T was isolated from a household dustbin on Brain Heart Infusion (BHI; BD) agar, glucose-yeast extract-malt extract agar (GYM; medium 65; DSMZ) and Columbia agar (BD) supplemented with 5 % sheep blood. The organism was grown on yeast extract-malt extract agar (ISP 2), oatmeal agar (ISP 3) and inorganic salts-starch agar (ISP 4) as described by Shirling & Gottlieb (1966) and examined for pigmentation and colour of aerial and substrate mycelia. Gram and ZiehlNeelsen stains were applied to hyphae grown on Columbia agar. Growth at different temperatures was determined at 10, 20, 27, 37 and 42 u C and a range of phenotypic characteristics was examined using standard procedures (Gordon, 1966(Gordon, , 1967Gordon & Mihm, 1957;Yassin et al., 1995). Tolerance to NaCl (0-16 %) was tested in GYM broth. Biomass for chemotaxonomic studies was derived from a 7-day-old BHI broth shake culture incubated at 37 u C, harvested by centrifugation and washed with distilled water. Standard procedures were used to determine the isomeric form of diaminopimelic acid (Becker et al., 1964), whole-cell sugars (Lechevalier, 1968), nonhydroxylated fatty acids (Minnikin et al., 1980;Yassin et al., 2007), polar lipids (Yassin et al., 1993b) and isoprenoid quinones (Collins et al., 1977;Yassin & Hupfer, 2006). Genomic DNA extraction and PCR-mediated amplification of the 16S rRNA gene sequence were carried out using established procedures (Rainey et al., 1996). The purified PCR products were sequenced using a Taq DyeDeoxy...

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Tsukamurella inchonensis sp. nov.

Cited by 92 publications

References 34 publications

Corynebacterium aurimucosum sp. nov. and emended description of Corynebacterium minutissimum Collins and Jones (1983).

Corynebacterium aurimucosum sp. nov. and emended description of Corynebacterium minutissimum Collins and Jones (1983).

Tsukamurella pulmonis Bloodstream Infection Identified by secA1 Gene Sequencing

Nocardiopsis potens sp. nov., isolated from household waste

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