There are still very few studies of the Torque Tenovirus infection rate between hemodialysis patients in Iraq. Thus, the aim of this study was to assess the frequency of TTV viremia in hemodialysis Iraqi patients. This study has carried out in Baghdad city from October 2019 to February 2020 and included 150 patients with kidney failure disease undergoing hemodialysis and 50 control subjects .Blood samples have been obtained for serological TTV detection. The result shows PCR has been used to detect TTV, isolates have been identified through N22 region sequencing and phylogenetic analysis has also been performed. Of the 150 patients, 2 (1 male and 1 female) were TTV positive.The results shows in ( isolate 14) substitution four Transversion A>C, C>G,A>T, and T>A three Transition T>C,A>G, and T>C of Specific region within TTV(N-22) and showed 94% identified with a standard in Gene Bank while having 99% identified with a standard in Gene Bank with (isolate 17) substitution fiveTransversion A>T,A>C,T>A, A>Tand C>G threeTransition A>G,A>G and T>C . The phylogenetic analysis showed that identical among themselves and the world with 100% compatibility values.