Tu1887 microRNAs Profiles of Mouse Acinar Cells and Its Implications in Acute Pancreatitis

Dixit, Ajay Kumar; Dawra, Rajinder; Barlass, Usman; Sareen, Archana; Yuan, Zubiao; Sarver, Anne E.; Subramanian, Subbaya; Saluja, Ashok

doi:10.1016/s0016-5085(15)33157-7

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“…11 Abnormal expression or dysfunction of miRNA may lead to many diseases such as tumor and pancreatitis. Emerging evidence has revealed that multiple miRNAs were significantly upregulated in the process of acinar cell injury in acute pancreatitis 12 . An increasing number of reports showed that miR-27a-5p was aberrant expressed in tissues of various diseases, such as pancreatitis, gastric cancer, 13 lung cancer, 14 liver cancer, 15 and was involved in the regulation of malignant biological characteristics of cells.…”

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confidence: 99%

Effect of microRNA‐27a‐5p on apoptosis and inflammatory response of pancreatic acinar cells in acute pancreatitis by targeting PTEN

Kong

Zhao

et al. 2019

J of Cellular Biochemistry

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To investigate the apoptosis and inflammatory response of microRNA‐27a‐5p (miR‐27a‐5p) in pancreatic acinar cells of acute pancreatitis (AP) and its related mechanisms. Rat pancreatic acinar cell line AR42J was treated with caerulein (10nmol/L) to construct an acute pancreatitis cell model. Quantitative real‐time polymerase chain reaction was performed to measure the expression of miR‐27a‐5p; The miR‐27a‐5p mimic was transfected into cell, and the apoptosis rate of the cells was detected by flow cytometry; The levels of TNF‐α, IL‐1, and IL‐6 in the culture supernatant were determined by enzyme‐linked immunosorbent assay; TargetScans database predicted and dual luciferase reporter gene assay verified the relationship between miR‐27a‐5p and the phosphatase and tensin homolog deleted on chromosome 10 (PTEN); The recovery experiment explored the apoptosis and the effects of inflammatory responses. The expression of miR‐27a‐5p decreased gradually (P < 0.05) and the expression of PTEN increased gradually (P < 0.05) with the prolongation of acting time. Upregulation of miR‐27a‐5p significantly promoted cell apoptosis (P < 0.05) and inhibited inflammatory response (P < 0.05); The TargetScans database predicted that the 3'UTR of PTEN contains a base complementary to the miR‐27a‐5p seed region. Cotransfection of wild‐type vector (PTEN‐WT) with miR‐27a‐5p mimic or miR‐27a‐5p inhibitor significantly affected the relative activity of luciferase (P < 0.05), and no significant impact was observed in mutant PTEN‐MUT. Compared with miR‐27a‐5p + pcDNA group, transfection of miR‐27a‐5p mimic and pcDNA‐PTEN significantly increased the expression of PTEN (P < 0.05), decreased the apoptotic rate (P < 0.05), and increased the inflammatory response (P < 0.05). miR‐27a‐5p induced apoptosis and inhibited the inflammatory response of pancreatic acinar cells in AP by targeting PTEN.

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