Tubal cytokine changes accompany the epithelial atypia of letrozole-stimulated ovaries

Abdel-Hamid, Ahmed A.M.; Mesbah, Yaser; Farouk, Mona F.M.

doi:10.1016/j.acthis.2016.01.004

Cited by 12 publications

(3 citation statements)

References 44 publications

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“…Ki67 has emerged as a reliable marker for evaluation of cell proliferation in reactive inflammation, allergy, vascular stress and in precancerous conditions . Mouse anti‐Ki67 antibodies (monoclonal, 1/25, Clone MIB‐5, Dako) were used to stain UB sections guided by the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Dysplastic urothelial changes accompany empagliflozin administration in urinary bladder of experimental diabetes

Abdel-Hamid

Firgany

2019

Int J Experimental Path

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Summary Empagliflozin (EMPA) is a promising novel antidiabetic drug; however, doubts have been raised regarding its use and the increased risk of urinary bladder carcinoma. In this study, we evaluated urothelium expression of cytokeratins (CKs) and Ki‐67 proliferative activity in the urinary bladder of diabetic (DM + EMPA) and non‐diabetic rats after EMPA administration. By routine histology, dysplastic changes were detected in the urothelium of diabetic as well as non‐diabetic animals after EMPA administration. Moreover, the expression of CK‐7 and CK‐8 was significantly decreased (P < .05) while that of CK‐20 as well as Ki‐67 was significantly increased (P < .05) in EMPA per se and DM + EMPA urothelium groups compared to that of control and diabetics. The dysplastic changes together with the increased proliferative activity in urothelium after EMPA administration provide a cellular evidence that supports the former clinical concerns.

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Section: Methodsmentioning

confidence: 99%

Dysplastic urothelial changes accompany empagliflozin administration in urinary bladder of experimental diabetes

Abdel-Hamid

Firgany

2019

Int J Experimental Path

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“…) as well as premalignant (Abdel‐Hamid et al . ,b) conditions; therefore, we employed Ki67 (monoclonal MIB‐1, dilution 1:50; DakoCytomation, Carpinteria, CA, USA) as an index for evaluating spermatagonial proliferation. Streptavidin–biotin technique was the selected method for immunohistochemistry, and visualization of the immunoreaction was attained by a horseradish peroxidase‐based chromogen substrate.…”

Section: Methodsmentioning

confidence: 99%

Correlation between testicular mast cell count and spermatogenic epithelium in non‐obstructive azoospermia

Abdel-Hamid

Atef

Zalata

et al. 2018

Int J Experimental Path

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Although there is emerging evidence that mast cells are involved in infertility, their exact role has not been elucidated clearly. Here we carried out a retrospective case-control study to find out whether there is a correlation between mast cell (MC) count and proliferation (Ki67 index) of the spermatogenic epithelium as well as of the Sertoli cells (vimentin-positive) in non-obstructive azoospermia (NOA). We assessed MCs, Ki67 and vimentin expression in Sertoli cells in testicular biopsies of germ cell aplasia (GCA, n = 14) and maturation arrest (MA, n = 14) vs. normal spermatogenesis (n = 14) cases. There was a significant decrease in the spermatogonial Ki67 index (1.25 ± 0.91, 4.21 ± 1.81 vs. 39.57 ± 3.92) and Johnsen score (2.48 ± 0.65, 4.89 ± 1.05 vs. 9.75 ± 0.30) as well as a significant increase (P < 0.001) in MC count (29.00 ± 4.11, 7.57 ± 1.95 vs. 3.00 ± 1.30) in seminiferous tubules of infertile cases with GCA and MA vs. controls. On the other hand, the percentage of vimentin-expressing Sertoli cells was significantly decreased (P < 0.001) in biopsies of cases with MA (35.50 ± 15.62) compared to those of cases with GCA and controls (72.64 ± 10.67 and 98.57 ± 1.45 respectively). Additionally, a significant negative correlation was detected between MC count and Ki67 index as well as Johnsen score in the MA group which became more significant in the GCA group. The significant increase in MC count in the GCA group and to a lesser extent in the MA group indicates their possible role in NOA particularly at the spermatogonial proliferation level and this is supported by the significant negative correlation with the Ki67 index.

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“…The role of local cytokines has been previously demonstrated in placental,[ 18 19 ] endometrial, tubal,[ 20 21 ] tissues, and extra-genital organs[ 22 23 ] both in experimental[ 24 25 ] and clinical studies. We evaluated IL-1β, IL-6 as well as TNF-α level in homogenates of placental bed (100 mL) by ELISA according to instructions of the manufacturer (Quantakine kits).…”

Section: S Ubjects and M Ethodsmentioning

confidence: 99%

Dominance of Pro-Inflammatory Cytokines Over Anti-Inflammatory Ones in Placental Bed of Creta Cases

Abdel-Hamid¹,

Mesbah²,

Soliman³

et al. 2022

Journal of Microscopy and Ultrastructure

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Objective: The objective of the study is to investigate changes occurring in key inflammatory cytokines at molecular level (including genetic and protein) in placental bed of placenta creta compared to that of normal placenta and their correlation to interstitial extravillous trophoblasts (EVT) number. Subjects and Methods: Case–control study including placentas of patients with invasive placentation (creta placentas, n = 19) compared with those of normal placentation (n = 19). Besides routine histology and immunocytochemistry detection (cytokeratin-7 [CK-7]), addition to biochemical evaluation of expression of various cytokines including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), IL6, IL-1RA, IL-8, IL-10, and IL-13 was carried out. Results: Routine histological examination of placentas of creta cases revealed CK-7+ extravillous trophoblasts (EVT) penetrating deeply the myometrium with various histopathological arrangements and trophoblastic vascular invasion of the deep myometrial blood vessels. A significant increase (P < 0.05) in the mRNA expression of TNF-α, IL-1 β, and IL6 with an insignificant decrease in placental bed IL-1RA, IL-8, IL-10, and IL-13 was observed in creta cases compared to the control ones. A corresponding significant increase was detected in the protein levels of TNF-α, IL-1 β, and IL-6 as well as an insignificant decrease in placental bed IL-1RA, IL-8, IL-10, and IL-13 in creta cases compared to the normal ones. Moreover, we displayed a significant positive correlation (P < 0.05) between interstitial EVT number and mRNA expression of almost all pro-inflammatory cytokines with negative but insignificant correlation with anti-inflammatory cytokines in creta cases. Conclusion: The upregulated pro-inflammatory cytokines and the correlation of their expression with the increased interstitial EVT provide a supporting evidence of their potentially more relevant role in the development of placenta creta than the anti-inflammatory ones.

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Tubal cytokine changes accompany the epithelial atypia of letrozole-stimulated ovaries

Cited by 12 publications

References 44 publications

Dysplastic urothelial changes accompany empagliflozin administration in urinary bladder of experimental diabetes

Dysplastic urothelial changes accompany empagliflozin administration in urinary bladder of experimental diabetes

Correlation between testicular mast cell count and spermatogenic epithelium in non‐obstructive azoospermia

Dominance of Pro-Inflammatory Cytokines Over Anti-Inflammatory Ones in Placental Bed of Creta Cases

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