Tubeimoside-1 induces G2/M phase arrest and apoptosis in SKOV-3 cells through increase of intracellular Ca2+ and caspase-dependent signaling pathways

Yang, Zhu

doi:10.3892/ijo.2011.1218

Cited by 16 publications

(6 citation statements)

References 24 publications

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“… 22 , 25 It has been reported that ERK1/2 regulates the expression of Bcl-2, which partly contributes to the presumption that ERK1/2 is the upstream effector of Bcl-2. 29 Consistent with the available studies, our data pointed out that various concentrations of TBMS1 increased c-PARP expression, accompanied by the decrease in total PARP expression after cells were treated with high doses of TBMS1, and TBMS1 markedly inhibited p-ERK1/2 expression, which leads to the Bcl-2 product decline in this study. Therefore, our results revealed that TBMS1 might induce intrinsic apoptosis through the ERK1/2/Bcl-2/caspase-9/caspase-3/PARP pathway, and the extrinsic apoptotic pathway was also involved in cellular apoptosis.…”

Section: Discussionsupporting

confidence: 93%

The effect of tubeimoside-1 on the proliferation, metastasis and apoptosis of oral squamous cell carcinoma in vitro

Cui

et al. 2018

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BackgroundTubeimoside-1 (TBMS1), a triterpenoid saponin extracted from traditional Chinese medicine tubeimoside, exerts a cytotoxic effect on several human cancer cell lines. However, no study has focused on whether TBMS1 works on oral squamous cell carcinoma (OSCC).Materials and methodsWe treated OSCC cells with TBMS1 to detect the effect and relevant molecular basis of TBMS1 for the first time. We chose two oral cancer cell lines, CAL27 and SCC15, for this study. First, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide assay and cell proliferation 5′-bromo-2′-deoxyuridine assay were carried out to detect cell growth. Second, colony formation assay was performed to assess clonogenesis capacity. Next apoptosis was analyzed by flow cytometry. Subsequently, wound healing and transwell assays were applied to explore cell migration. Finally, Western blot was further performed to examine corresponding proteins’ expression change.ResultsOur data showed that TBMS1 significantly suppressed proliferation of OSCC cells in a dose- and time-dependent manner and it inhibited migration of OSCC cells as well. After treatment with TBMS1, OSCC cells underwent cell apoptosis. Furthermore, Western blot demonstrated that TBMS1 downregulated apoptosis-associated proteins such as PARP, p-ERK1/2, Bcl-2, caspase-3, caspase-7 and caspase-8 and upregulated cleaved PARP, cleaved caspase-3 and cleaved caspase-9. It could also reduce expression of c-Myc and MMP-7. Meanwhile, TBMS1 did not change the total ERK1/2 expression.ConclusionThese results revealed that TBMS1 might be a potential chemotherapeutic drug for the management of OSCC.

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Section: Discussionsupporting

confidence: 93%

The effect of tubeimoside-1 on the proliferation, metastasis and apoptosis of oral squamous cell carcinoma in vitro

Cui

et al. 2018

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“… 7 , 8 TBMS1 induces G2/M phase arrest and apoptosis in ovarian cancer cells through an increase in intracellular Ca 2+ levels and caspase-dependent signaling. 9 TBMS1 inhibited human choriocarcinoma cancer cell proliferation by inducing Cytochrome C release and apoptosis via the mitochondrial-related signaling pathway. 10 TBMS1 inhibits proliferation and induces apoptosis by increasing the Bax to Bcl-2 ratio and decreasing COX-2 expression in lung cancer cells.…”

Section: Introductionmentioning

confidence: 94%

Tubeimoside-1 induces glioma apoptosis through regulation of Bax/Bcl-2 and the ROS/Cytochrome C/Caspase-3 pathway

Geng

Wang

et al. 2015

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BackgroundTubeimoside-1 (TBMS1) is a natural compound isolated from tubeimoside, which has been widely used as a traditional Chinese herbal medicine. The purpose of the present study is to investigate the anti-tumor effect and the underling mechanism of TBMS1 on glioma cancer cells.MethodsThe MTT assay was performed to evaluate the effect of TBMS1 on glioma cell proliferation. The fluorescent microscopy and flow cytometry analysis were performed to evaluate the effect of TBMS1 on glioma cell apoptosis. The Western blot analysis was used to evaluate the protein change.ResultsTBMS1 inhibited glioma cancer cell proliferation in a dose- and time-dependent manner. Fluorescent microscopy and flow cytometry analysis demonstrated that TBMS1 induced glioma cell apoptosis in a concentration-dependent manner. Western blotting showed that TBMS1 induced apoptosis by increasing the expression of Bax and downregulating the level of Bcl-2. Furthermore, we found that TBMS1 induced apoptosis by increasing the concentration of reactive oxygen species through the release of Cytochrome C and activation of Caspase-3.ConclusionThese findings indicate that TBMS1 may be developed as a possible therapeutic agent for the management of glioma.

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“…Tubeimoside-1 can also sensitize cell response to cisplatin in cisplatin-resistant human ovarian cancer cells (A2780/DDP) through down-regulation of ERK and up-regulation of p38 (Liu H. Z. et al, 2011). Tubeimoside-1 increased the expression of CHOP and phosphorylated p38, resulting in G 2 /M phase arrest and apoptosis in SKOV-3 human ovarian carcinoma cells (Chen W. J. et al, 2012). In addition, tubeimoside-1 can induce oxidative stress-mediated apoptosis and G 2 /M phase arrest in HepG2 liver cancer cells via NF-κB, JNK, and p53 pathways (Yin et al, 2011).…”

Section: Components Isolated From Tcms That Inhibit Mapkmentioning

confidence: 99%

Development of Certain Protein Kinase Inhibitors with the Components from Traditional Chinese Medicine

et al. 2017

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Traditional Chinese medicines (TCMs) have been used in China for more than two thousand years, and some of them have been confirmed to be effective in cancer treatment. Protein kinases play critical roles in control of cell growth, proliferation, migration, survival, and angiogenesis and mediate their biological effects through their catalytic activity. In recent years, numerous protein kinase inhibitors have been developed and are being used clinically. Anticancer TCMs represent a large class of bioactive substances, and some of them display anticancer activity via inhibiting protein kinases to affect the phosphoinositide 3-kinase, serine/threonine-specific protein kinases, pechanistic target of rapamycin (PI3K/AKT/mTOR), P38, mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinases (ERK) pathways. In the present article, we comprehensively reviewed several components isolated from anticancer TCMs that exhibited significantly inhibitory activity toward a range of protein kinases. These components, which belong to diverse structural classes, are reviewed herein, based upon the kinases that they inhibit. The prospects and problems in development of the anticancer TCMs are also discussed.

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Tubeimoside-1 induces G2/M phase arrest and apoptosis in SKOV-3 cells through increase of intracellular Ca2+ and caspase-dependent signaling pathways

Cited by 16 publications

References 24 publications

The effect of tubeimoside-1 on the proliferation, metastasis and apoptosis of oral squamous cell carcinoma in vitro

The effect of tubeimoside-1 on the proliferation, metastasis and apoptosis of oral squamous cell carcinoma in vitro

Tubeimoside-1 induces glioma apoptosis through regulation of Bax/Bcl-2 and the ROS/Cytochrome C/Caspase-3 pathway

Development of Certain Protein Kinase Inhibitors with the Components from Traditional Chinese Medicine

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