Tuber Extract Mediated Biosynthesis of Silver Nanoparticles and its Antioxidant, Antibacterial Activity

Abstract

A B S T R A C TThe biosynthesis of silver nanoparticles by the reduction of aqueous silver metal ions during revelation to both fresh, dry tuber extract and in vitro antioxidant, antibacterial activity of sweet potato were studied. Tuber extract of sweet potato was prepared for the synthesis of silver nanoparticles under different reaction time. The prepared materials were characterized by UV-visible spectroscopy, X-Ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FT-IR). During incubation period, the intensity of colour increased and its characteristic absorption was recorded at 428 and 439 nm. X-ray diffraction showed that the average particles size 4.77 and 5.6 nm. The FT-IR spectra of the tuber extract and synthesized silver nanoparticles revealed the reducing and capping actions of biomolecules such as amine, peptide, amide, lactone, polyphenol groups in protein linkages and it was found to be involved in the stabilization and biosynthesis of AgNPs. Scanning Electron Microscopy (SEM) showed silver nanoparticles was pure and the sizes were ranging from 1-10 µm. Green synthesized silver nanoparticles exhibited strong antioxidant and effective antibacterial activity against human pathogenic bacteria. Thus, green synthesis seems to be cost effective and alternate to conventional methods of silver nanoparticles synthesis. No synthetic reagent were used in this investigation and thus it is an environmentally safe method with potential for biomedical and agriculture application.

Green synthesis of silver nanoparticles using Eulophia herbacea (Lindl.) tuber extract and evaluation of its biological and catalytic activity

“…1). This method requires lesser time as compared to earlier reports on AgNPs synthesis using plant extracts [4,7,8,[25][26][27]. Different phytoconstituents were found in the tuber extract (Table 1).…”

“…The synthesized AgNPs by E. Herbacea tuber extract acted as a catalyst, increasing the rate of reduction of methylene blue and Congo red. The earlier methods of AgNPs synthesis by tubers were limited to biological activities and does not explored dye reduction studies [4,7,8]. The maximum absorption of MB (a thiazine dye) in the UV-visible region was at a wavelength of 664 nm and that of CR (an azo dye) was at 338 mm and 498 nm.…”

“…The tubers of Curcuma longa [4,7], sweet potato [8], Dioscorea bulbifera, Dioscorea batatas, Dioscorea oppositifolia, etc. are rich with different reducing and capping agents that generate stable metal nanoparticles [9][10][11].…”

Phytochemical Analysis and Evaluation of Antimicrobial Activity in the Whole Plant Extracts of Gloriosa Superba

“…The organic solvent of the plant extract (tuber) renders greater antibacterial ability than the Phenolic compounds shoot and flower extract. This result can be attributed to the polarity of the solvent used for extraction, essential bioactivity, and capability to diffuse in media utilized in assay [31,32]. Therefore, in many elements of the plant for herbal safety against microbial infection are packed with phytochemical substances.…”

Evaluation of Antimicrobial Activity and Phytochemicals Analysis of Whole Plant Extract of Vinca Rosea

“…The organic solvent of the whole plant extract (shoot) render greater antibacterial ability than the shoot and flower extract. This result can be recognized to the polarity of the solvent used for extraction, essential bioactivity, and ability to diffuse in media utilized in assay [36,37].…”