Tuber pulchrosporum sp. nov., a black truffle of the Aestivum clade (Tuberaceae, Pezizales) from the Balkan peninsula

Polemis, Elias; Konstantinidis, Georgios; Fryssouli, Vassiliki; Slavova, Monica; Tsampazis, Triantafyllos; Nakas, Vasileios; Assyov, Boris; Kaounas, Vasileios; Zervakis, Georgios I.

doi:10.3897/mycokeys.47.32085

Cited by 8 publications

(2 citation statements)

References 48 publications

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“…Therefore, we have only presented the phylogram obtained from the ML analysis ( Figure 5 ). Six main clades, namely Gibbosum, Puberulum, Latisporum, Maculatum, Rufum, and Melanosporum, were assigned according to the findings of previous phylogenetic studies [ 3 , 61 , 62 ]. Our phylogenetic results place all the specimens obtained from each location within the monophyletic clade of T. thailandicum in the Gibbosum clade with high support values (100% BS and 1.0 PP).…”

Section: Resultsmentioning

confidence: 99%

Soil Metabarcoding Offers a New Tool for the Investigation and Hunting of Truffles in Northern Thailand

Suwannarach

Kumla

In-On

et al. 2021

JoF

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Truffles (Tuber spp.) are well-known as edible ectomycorrhizal mushrooms, and some species are one of the most expensive foods in the world. During the fruiting process, truffles produce hypogeous ascocarps; a trained pig or dog is needed to locate the ascocarps under the ground. Truffles in northern Thailand have been recorded in association with Betulaalnoides and Carpinus poilanei. In this study, we investigated the soil mycobiota diversity of soil samples from both of these truffle host plants in native forests using environmental DNA metabarcoding to target the internal transcribed spacer 1 (ITS1) region of the rDNA gene for the purposes of investigation of truffle diversity and locating truffles during the non-fruiting phase. In this study, a total of 38 soil samples were collected from different locations. Of these, truffles had been found at three of these locations. Subsequently, a total of 1341 putative taxonomic units (OTUs) were obtained. The overall fungal community was dominated by phylum-level sequences assigned to Ascomycota (57.63%), Basidiomycota (37.26%), Blastocladiomycota (0.007%), Chytridiomycota (0.21%), Glomeromycota (0.01%), Kickxellomycota (0.01%), Mortierellomycota (2.08%), Mucoromycota (0.24%), Rozellomycota (0.01%), Zoopagomycota (0.003%), and unidentified (2.54%). The results revealed that six OTUs were determined to be representative and belonged to the genus Tuber. OTU162, OTU187, OTU447, and OTU530 belonged to T. thailandicum, T. lannaense, T. bomiense, and T. magnatum, whereas OTU105 and OTU720 were acknowledged as unrecognized Tuber species. From 38 locations, OTUs of truffles were found in 33 locations (including three previously known truffle locations). Thus, 30 collection sites were considered new locations for T. thailandicum, T. bomiense, and other unrecognized Tuber species. Interestingly, at 16 new locations, mature ascocarps of truffles that were undergoing the fruiting phase were located underground. All 16 truffle samples were identified as T. thailandicum based on morphological characteristics and molecular phylogenetic analysis. However, ascocarps of other truffle species were not found at the new OTUs representative locations. The knowledge gained from this study can be used to lead researchers to a better understanding of the occurrence of truffles using soil mycobiota diversity investigation. The outcomes of this study will be particularly beneficial with respect to the search and hunt for truffles without the need for trained animals. In addition, the findings of this study will be useful for the management and conservation of truffle habitats in northern Thailand.

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Section: Resultsmentioning

confidence: 99%

Soil Metabarcoding Offers a New Tool for the Investigation and Hunting of Truffles in Northern Thailand

Suwannarach

Kumla

In-On

et al. 2021

JoF

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“…The Tuber genus is a group of important ectomycorrhizal fungi, which comprises numerous recognized species ( Wan et al, 2016 ; Guevara-Guerrero et al, 2018 ; Polemis et al, 2019 ). Limited morphological features and overlapping of some features render it difficult to differentiate some Tuber species accurately.…”

Section: Discussionmentioning

confidence: 99%

The 287,403 bp Mitochondrial Genome of Ectomycorrhizal Fungus Tuber calosporum Reveals Intron Expansion, tRNA Loss, and Gene Rearrangement

Bao

et al. 2020

Front. Microbiol.

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In the present study, the mitogenome of Tuber calosporum was assembled and analyzed. The mitogenome of T. calosporum comprises 15 conserved protein-coding genes, two rRNA genes, and 14 tRNAs, with a total size of 287,403 bp. Fifty-eight introns with 170 intronic open reading frames were detected in the T. calosporum mitogenome. The intronic region occupied 69.41% of the T. calosporum mitogenome, which contributed to the T. calosporum mitogenome significantly expand relative to most fungal species. Comparative mitogenomic analysis revealed large-scale gene rearrangements occurred in the mitogenome of T. calosporum, involving gene relocations and position exchanges. The mitogenome of T. calosporum was found to have lost several tRNA genes encoding for cysteine, aspartate, histidine, etc. In addition, a pair of fragments with a total length of 32.91 kb in both the nuclear and mitochondrial genomes of T. calosporum was detected, indicating possible gene transfer events. A total of 12.83% intragenomic duplications were detected in the T. calosporum mitogenome. Phylogenetic analysis based on mitochondrial gene datasets obtained well-supported tree topologies, indicating that mitochondrial genes could be reliable molecular markers for phylogenetic analyses of Ascomycota. This study served as the first report on mitogenome in the family Tuberaceae, thereby laying the groundwork for our understanding of the evolution, phylogeny, and population genetics of these important ectomycorrhizal fungi.

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Truffles: Biodiversity, Ecological Significances, and Biotechnological Applications

et al. 2021

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Tuber pulchrosporum sp. nov., a black truffle of the Aestivum clade (Tuberaceae, Pezizales) from the Balkan peninsula

Cited by 8 publications

References 48 publications

Soil Metabarcoding Offers a New Tool for the Investigation and Hunting of Truffles in Northern Thailand

Soil Metabarcoding Offers a New Tool for the Investigation and Hunting of Truffles in Northern Thailand

The 287,403 bp Mitochondrial Genome of Ectomycorrhizal Fungus Tuber calosporum Reveals Intron Expansion, tRNA Loss, and Gene Rearrangement

Truffles: Biodiversity, Ecological Significances, and Biotechnological Applications

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