Tuber spp. biodiversity in one of the southernmost European distribution areas

Pomarico, Maria; Figliuolo, Giovanni; Rana, Gian Luigi

doi:10.1007/978-1-4020-6865-2_15

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…Tuber aestivum (summer truffle) has been frequently overlooked in most European countries. Nowadays, it has been rediscovered in a number of habitats all over Europe (Chevalier & Frochot, 1997;Montecchi & Sarasini, 2000, Ga&o et al, 2005, Pomarico et al, 2007 and is considered the most common European truffle. Soil and climatic requirements of the summer truffle can be met in many natural localities in Europe and this fungus is thus probably the easiest of all truffles to cultivate commercially.…”

Section: Introductionmentioning

confidence: 99%

Detection of summer truffle (Tuber aestivum Vittad.) in ectomycorrhizae and in soil using specific primers

Gryndler

Hršelová

Soukupová

et al. 2011

FEMS Microbiology Letters

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Tuber aestivum is becoming an important commodity of great economical value in some European countries. At the same time, it is a highly protected organism in other countries, where it needs careful treatment. A reliable method of detection in roots and soil is thus needed for assessment of geographic distribution, ecological studies and inoculation efficiency testing in man-made experiments. A PCR-based method of detection of T. aestivum using specific primers was therefore developed. A pair of PCR primers Tu1sekvF/Tu2sekvR selective for T. aestivum and some genotypes of Tuber mesentericum was designed on the basis of the known internal transcribed spacer T. aestivum sequences. TaiI restriction cleavage was then used to distinguish the two species. The selectivity of the designed primer pair was evaluated using DNA extracted from specimens of a further 13 Tuber spp. Subsequently, the selectivity and robustness to false-positive results with nontarget DNA of the designed primers was compared with two other primer pairs (UncI/UncII and BTAE-F/BTAEMB-R). The occurrence of T. aestivum in soil and ectomycorrhizae collected in its native habitat has been successfully detected using the designed primers and nested PCR. The method is reliable and thus suitable for detection of T. aestivum in the field.

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Section: Introductionmentioning

confidence: 99%

Detection of summer truffle (Tuber aestivum Vittad.) in ectomycorrhizae and in soil using specific primers

Gryndler

Hršelová

Soukupová

et al. 2011

FEMS Microbiology Letters

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“…The current lack of such information is mainly due to a paucity of dedicated, large-scale studies employing molecular detection to address truffle communities in soils (Leonardi et al, 2013;Taschen et al, 2016). This means most of the current knowledge is reliant on information from fruiting body collections or spatially restricted molecular studies (e.g., Berch & Bonito, 2016;Marjanovic, Grebenc, Markovic, Glisic, & Milenkovic, 2010;Pomarico, Figliuolo, & Rana, 2007).…”

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confidence: 99%

Truffle biogeography—A case study revealing ecological niche separation of different Tuber species

Gryndler

Šmilauer

Šťovíček

et al. 2017

Ecology and Evolution

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Ecology of hypogeic mycorrhizal fungi, such as truffles, remains largely unknown, both in terms of their geographical distribution and their environmental niches. Occurrence of true truffles (Tuber spp.) was therefore screened using specific polymerase chain reaction (PCR) assays and subsequent PCR amplicon sequencing in tree roots collected at 322 field sites across the Czech Republic. These sites spanned a wide range of climatic and soil conditions. The sampling was a priori restricted to areas thought to be suitable for Tuber spp. inasmuch as they were characterized by weakly acidic to alkaline soils, warmer climate, and with tree species previously known to host true truffles. Eight operational taxonomic units (OTUs) corresponding to Tuber aestivum, T. borchii, T. foetidum, T. rufum, T. indicum, T. huidongense, T. dryophilum, and T. oligospermum were detected. Among these, T. borchii was the OTU encountered most frequently. It was detected at nearly 19% of the sites. Soil pH was the most important predictor of Tuber spp. distribution. Tuber borchii preferred weakly acidic soils, T. foetidum and T. rufum were most abundant in neutral soils, and T. huidongense was restricted to alkaline soils. Distribution of T. aestivum was mainly dictated by climate, with its range restricted to the warmest sites. Host preferences of the individual Tuber spp. were weak compared to soil and climatic predictors, with the notable exception that T. foetidum appeared to avoid oak trees. Our results open the way to better understanding truffle ecology and, through this new knowledge, also to better‐informed trufficulture.

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