Tuberculosis treatment effect on T-cell interferon-γ responses to<i>Mycobacterium tuberculosis-</i>specific antigens

Chee, C. B. E.; KhinMar, Kyi Win; Gan, Suay Hong; Barkham, Timothy; Koh, Chwee Kim; Shen, L; Wang, Y T

doi:10.1183/09031936.00151309

Cited by 89 publications

(60 citation statements)

References 25 publications

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“…This would tend to underestimate the true burden of disease caused by infection with M. tuberculosis. Also there could be an underestimation of the risk of infection due to prior prophylactic treatment given during earlier TB screening surveys as prior treatment can cause children to revert to a negative TST or a negative QFN [22].…”

Section: Discussionmentioning

confidence: 99%

Ongoing tuberculosis transmission to children in Greenland

Søborg¹,

Koch²,

Thomsen³

et al. 2010

Eur Respir J

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Inuit in the Arctic are experiencing an increase in tuberculosis cases, reaching levels in Greenland comparable to high-incidence countries. This prompted us to study the level of tuberculosis transmission to Greenlandic children. Specifically, we estimated the current prevalence of Mycobacterium tuberculosis infection (MTI) and the underlying annual risk of MTI.2,231 Greenlandic school children aged 5-17 yrs (,25% of the Greenlandic population in the relevant age group) were tested for MTI using the tuberculin skin test and the QuantiFERON1-TB Gold in-tube test. Subjects with dual-positive results were considered infected and subjects with dual-negative results uninfected. The children with discordant test results were classified as probably having MTI and analysed separately.8.1% of the children had dual-positive test results. The annual risk of MTI was estimated as 0.80% (95% CI 0.67-0.92%) giving a cumulative risk at the 18th birthday of 13.4%. The annual risk of MTI varied substantially by ethnicity (0.87% in Inuit children, 0.02% in non-Inuit children; p,0.001) and by location (0.13% on the west coast, 1.68% on the south coast; p,0.001).M. tuberculosis transmission occurs at a very high level in Inuit children with pronounced geographic differences emphasising the need for immediate public health interventions.

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Section: Discussionmentioning

confidence: 99%

Ongoing tuberculosis transmission to children in Greenland

Søborg¹,

Koch²,

Thomsen³

et al. 2010

Eur Respir J

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“…The sensitivity, specificity, PPV, and NPV of the T-SPOT.TB test for the diagnosis of ATB are 84.95%, 85.12%, 82.94%, and 86.93%, respectively, according to recent research (4,8,12,15,16). When comparing the SFCs of the ATB and NATB groups, the median number of SFCs in the ATB group was significantly higher than that in the NATB group (P Ͻ 0.0001); in ATB patients, EPTB patients made up a significantly higher proportion than PTB patients (73.58% versus 26.42%; P Ͻ 0.05).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Characteristics of the Enzyme-Linked Immunospot Assay for Diagnosis of Active Tuberculosis in China

Wang¹,

Yu²,

Chen³

et al. 2015

Clin. Vaccine Immunol.

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bThe purpose of this study was to evaluate the characteristics of the T-SPOT.TB test for the diagnosis of active tuberculosis (ATB) and to distinguish ATB from other diseases using a receiver operating characteristic (ROC) curve. A total of 535 patients with suspected active tuberculosis were enrolled in the study and divided into ATB and nonactive tuberculosis (NATB) groups, as well as pulmonary tuberculosis (PTB) and extrapulmonary tuberculosis (EPTB) subgroups. The sensitivity, specificity, positive predictive value, negative predictive value, positive likelihood ratio, and negative likelihood ratio of the T-SPOT.TB test for the diagnosis of ATB were 84.95%, 85.12%, 82.94%, 86.93%, 5.71, and 0.18, respectively. The median number of spot-forming cells (SFCs) in the ATB group was higher than that in the NATB group (71 versus 1; P < 0.0001). The sensitivities in the PTB and EPTB subgroups were 92.31% and 81.77%. The areas under the curve (AUC) for the diagnosis of ATB using the T-SPOT.TB, early secreted antigenic target 6 (ESAT-6), and culture filtrate protein 10 (CFP-10) were 0.906, 0.884, and 0.877, respectively. A cutoff of 42.5 SFCs for ATB yielded a positive predictive value of 100%. Our study shows that the T-SPOT.TB test is useful for the diagnosis of ATB. Utilizing an ROC curve to select an appropriate cutoff made it possible to discriminate ATB from NATB.T uberculosis is a serious public health issue. Data from 202 countries and territories have shown that approximately 9 million people developed tuberculosis and 1.5 million people died from the disease in 2013 (1). China has the second heaviest burden of tuberculosis in the world, with 1 million new cases of active tuberculosis (ATB) each year and a reported Mycobacterium tuberculosis infection rate of 44.5% (2). The fifth national tuberculosis epidemiological survey in 2010 in China showed that the prevalence of active pulmonary tuberculosis (PTB) was 459/ 100,000 and the prevalence of smear-positive PTB was 66/100,000 in people over 15 years of age (3). At present, routine diagnostic methods for ATB in China include acid-fast bacillus smear, culture, pathology, erythrocyte sedimentation rate (ESR), and the tuberculin skin test (TST). However, as these methods have either low sensitivity or specificity or require a long time to provide a result, their clinical application has been limited.After M. tuberculosis infection, effector T cells targeting M. tuberculosis are generated in peripheral blood mononuclear cells (PBMCs) or body fluid mononuclear cells (MCs). These effector T cells secrete interferon gamma (IFN-␥) when stimulated by specific RD1 antigens, such as early secreted antigenic target 6 (ESAT-6) and culture filtrate protein 10 (CFP-10) (4-8), but these RD1 antigens are not present in Mycobacterium bovis bacillus Calmette-Guérin or in most environmental mycobacteria. The T-SPOT.TB test uses ESAT-6 and CFP-10 to stimulate effector T cells in the sample, which then secrete IFN-␥. The cytokine is then captured by specific antibodies in microtit...

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“…[8][9][10] . Interferon (IFN)-γ is a potent cytokine that indicates antimicrobial effect and also 40. modulates the production or activities of several cytokines and chemokines [11][12][13] …”

Section: Mycobacterium Tuberculosis (M Tuberculosis) Infection or Knmentioning

confidence: 99%

“…Predominantly IFN-γ is also contributed to less 134. severe forms of pulmonary TB 23 . Moreover, IFN-γ also enhances the antigen pre 135. sentation through the induction of the expression of molecules from the major 136. histocompatibility complex (MHC) class I and class II and promoting the differentiation 137. of CD4 T lymphocytes to the Th1 subpopulation 11,22 . Furthermore as conclusion in 138. this study MET associated to inflammation regulatory in DM-TB coinfection patients.…”

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confidence: 99%