Tuberostemonine N, an active compound isolated from Stemona tuberosa, suppresses cigarette smoke-induced sub-acute lung inflammation in mice

Jung, Kyung Hwa; Kil, Yun Seo; Jung, Jae Hyun; Park, Soojin; Shin, Dasom; Lee, Kyeseok; Seo, Eun Kyoung; Bae, Hyunsu

doi:10.1016/j.phymed.2015.11.015

Cited by 29 publications

(17 citation statements)

References 44 publications

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“…First, we wanted to examine whether CS exposure could induce Crohn’s disease-like illness. Therefore, we exposed the mice to CS as described in a previous study ( 18 ) and evaluated the impact of CS exposure in the bowel. The colon length was significantly shorter (air group: 77.1 ± 2.3 mm vs. CS group: 56.8 ± 0.8 mm, Figure 1 A) and the body weight was markedly reduced (Figure 1 B) by CS exposure after 4 weeks of CS exposure.…”

Section: Resultsmentioning

confidence: 99%

“…This study was performed using previously described experimental procedures, which expose mice to mainstream of CS ( 17 , 18 ). The mice were exposed to the smoke from five consecutive non-filtered cigarettes (Reference 3R4F without filter; University of Kentucky, Lexington, KY, USA) four times a day, 6 days per week, using a smoking apparatus described by previously ( 18 ). Female C57BL/6J WT, T-bet −/− , IFN-γ −/− , and WT EGFP mice were exposed to CS for 4 weeks, and CD4 + and CD8 + depletion, immune cell profiling in lamina propria (LP), hemoccult test and A4B7 + cell analysis studies were performed under 2 week CS exposure.…”

Section: Methodsmentioning

confidence: 99%

“…A section of the lung (4-µm thick) and transverse colons (7-µm thick) from each mouse were stained with hematoxylin and eosin and visualized on an Olympus BX51 microscope (Olympus, Tokyo, Japan) equipped with a DP71 digital camera (Olympus). A total histological score of the lung was calculated for each mouse as described previously ( 18 ). Briefly, the lung sections were scored from 0 to 5 by readers according to the following criteria: 0 = normal; 1 = very mild; 2 = mild; 3 = moderate; 4 = marked; 5 = severe inflammation.…”

Section: Methodsmentioning

confidence: 99%

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Cigarette Smoking Triggers Colitis by IFN-γ+ CD4+ T Cells

et al. 2017

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The increased incidence of Crohn’s disease in smokers has been recently reported, suggesting a strong association of cigarette smoke (CS) with colitis. However, the mechanism of the action of CS on colitis has not yet been explored. Here, we demonstrate that CS exposure is sufficient to induce colitis in mice. Interestingly, the colitis is mainly mediated by Th1, but not Th17, responses. CD4+ T-cell depletion or T-bet/IFN-γ deficiency protects against the development of colitis induced by CS. Additionally, IFN-γ-producing CD4+ T cells play a substantial role in CS-induced colitis. The adoptive transfer (AT) of effector T cells from CS-exposed WT mice into colitis-prone mice caused these mice to develop colitis, while the AT of effector T cells from IFN-γ knock-out mice did not. These findings have implications for broadening our understanding of CS-induced pathology and for the development of novel therapeutic strategies to treat Crohn’s disease.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Cigarette Smoking Triggers Colitis by IFN-γ+ CD4+ T Cells

et al. 2017

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“…Preliminary HPLC-ESI-MS analysis let expect that stichoneurine derivatives with stemoninine (21) as major component together with croomine (1) were responsible for the anti-inflammatory effect (Lim et al 2015). In a following study it was shown that tuberostemonine N (56) significantly inhibited the secretion of proinflammatory cytokines and chemokines in bronchoalveolar lavage fluid and decreased alveoli size in lung tissue (Jung et al 2016). A reduction of LPS-induced NO was also observed with a methanolic root extract of S. tuberosa in murine BV2 microglia cells.…”

Section: Anti-inflammatory Activitiesmentioning

confidence: 99%

“…Following the first report on multidrug resistance properties of an ethanolic extract of Stemona aphylla (Limtrakul et al 2007), a series of studies evaluated the synergistic growth inhibitory effect of Stemona alkaloids with cancer chemotherapeutic agents (Chanmahasathien et al 2011a, b;Umsumarng et al 2013Umsumarng et al , 2015Umsumarng et al , 2017Umsumarng et al , 2018. Furthermore, their anti- inflammatory activity was investigated and regarded as an inhibition of the expression of diverse inflammatory mediators (Hosoya et al 2011;Lim et al 2015;Jung et al 2016;Lee et al 2016;Wu et al 2018). The present review also reports on the nematicidal activity (Huang et al 2016;Chen et al 2018) and other miscellaneous properties as well as on the pharmacokinetics of that class of alkaloids (Dong et al 2012;Sun et al 2012;Han et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Structural classification and biological activities of Stemona alkaloids

Greger

2019

Phytochem Rev

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Stemona alkaloids represent a unique class of natural products exclusively known from the three genera Stemona, Stichoneuron, and Croomia of the monocotyledonous family Stemonaceae. Structurally they are characterized by a central pyrroloazepine core usually linked with two butyrolactone rings. The great diversity, comprising 215 derivatives, is created by the formation of additional CC linkages and oxygen bridges together with ring cleavages and eliminations of the lactone rings. Based on biosynthetic considerations they can be grouped into three structural types represented by the croomine, stichoneurine, and protostemonine skeleton. Due to the formation of characteristic terminal lactone rings and the central pyrrolidine ring pandanamine and pandamarilactonine, isolated from Stichoneuron calcicola, were suggested to represent biogenetic precursors. The taxonomically complex S. tuberosa group can be clearly segregated by the formation of stichoneurines, while the other Stemona species are characterized by protostemonine derivatives. Croomine derivatives are more widespread found in both groups and in the genus Croomia. Of chemotaxonomic significance are the different transformations of protostemonine into stemofolines with a cage-type structure or into pyridoazepines characterized by a six-membered piperidine ring. Stimulated by the great interest in the antitussive activity of Stemona alkaloids, differences in transport mechanisms and potencies via different administrative routes were investigated. Follow-up studies on the significant insecticidal activities focused on the mode of action by using biochemical and electrophysiological approaches. Screening for acetylcholinesterase inhibitory properties revealed a much higher potency for stemofoline derivatives compared to pyridoazepines, but showed also significant activity for isomers of stenine with a stichoneurine skeleton. Evaluating synergistic growth inhibitory effects with cancer chemotherapeutic agents stemofoline exhibited the most potent effect in the reversal of permeability glycoprotein-mediated multidrug resistance. The anti-inflammatory activity of some derivatives was identified as an inhibition of the expression of inflammatory mediators. Comparing the diverse bioactivities of Stemona alkaloids stemofoline-type derivatives are the most versatile compounds representing promising lead structures for further development as commercial agents in agriculture and medicine.

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HPLC/ESI‐QTOF‐MS/MS based untargeted metabolomics authentication of Taxus × media six tissues

Bai,

2024

Phytochemical Analysis

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IntroductionTaxus media (Taxus × media Rehder) is renowned for its high paclitaxel content, serving as a major source for industrial paclitaxel production. In addition to paclitaxel, T. media contains a diverse range of metabolites, including flavonoids, alkaloids, and terpenoids, which have been shown to possess antioxidant, antibacterial, anti‐inflammatory, and immunomodulatory effects. However, these compounds have not been thoroughly studied as key metabolites in T. media.ObjectiveThe untargeted metabolomics analysis of six T. media tissues provides new insights into the development and utilization of T. media metabolites.MethodThe extracts from six tissues of T. media were analyzed and subjected to analysis using high‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry (HPLC‐Q‐TOF‐MS/MS) and chemometric techniques.ResultsUsing a reliable HPLC‐Q‐TOF‐MS/MS method, we identified 312 compounds in six T. media tissues, including 214 previously unreported in T. media. To identify characteristic compounds across different tissues, 34 metabolites were further screened. KEGG metabolic pathway analysis revealed that these compounds primarily occur in the metabolic pathways of terpene glycosides, flavans, and O‐methylated flavonoids.ConclusionThis study initially utilized an HPLC‐QTOF‐MS/MS‐based metabolomics approach to assess the metabolites in different tissues of T. media, providing a basis for their utilization and management.

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Tuberostemonine N, an active compound isolated from Stemona tuberosa, suppresses cigarette smoke-induced sub-acute lung inflammation in mice

Cited by 29 publications

References 44 publications

Cigarette Smoking Triggers Colitis by IFN-γ+ CD4+ T Cells

Cigarette Smoking Triggers Colitis by IFN-γ+ CD4+ T Cells

Structural classification and biological activities of Stemona alkaloids

HPLC/ESI‐QTOF‐MS/MS based untargeted metabolomics authentication of Taxus × media six tissues

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