Tuberous sclerosis complex 1 (PvTSC1) participates in ammonia nitrogen induced oxidative stress in Penaeus vannamei by regulating autophagy

Wang, Feifei; Liang, QingJian; Liu, Can; Dong, Wenna; Ou, MuFei; Li, Zhonghua; Liu, Yuan; Wang, Weina

doi:10.1016/j.aquaculture.2020.736107

Cited by 9 publications

(3 citation statements)

References 41 publications

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“…Rapamycin is an inhibitor of the mTOR signaling pathway and is usually used as an inducer of autophagy (Sotthibundhu, et al 2016). Our previous studies have shown that autophagy plays an important role in the environmental stress of shrimp (Liang, et al 2021a;Liang, et al 2021b;Wang, et al 2020;Wang, et al 2021). In this study, we aimed to explore the role of autophagy in shrimp response to the environment by administering rapamycin intramuscularly to shrimp and to understand the molecular mechanisms of autophagy to expand our understanding of shrimp adaptation to environmental challenges.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome revealed the role of autophagy in nitrite oxidative stress of Penaeus vannamei

Lin,

Liu,

Chen

et al. 2024

Preprint

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Nitrite constitutes a significant pollutant that impedes the growth of P. vannamei. Conventional treatments are primarily geared toward decreasing nitrite concentrations from an environmental standpoint. Nevertheless, investigating methodologies to enhance the ability of shrimp to withstand nitrite-induced stresses on an individual basis remains an unexplored topic. The present study examines the impact of autophagy on shrimp’s response to high nitrite environments through rapamycin injection. The research findings indicate that activating autophagy can effectively enhance the survival rate of shrimp under high nitrite conditions. Additionally, total hemocyte count (THC) results in the hemolymph demonstrate that autophagy can alleviate the reduction in blood cell count caused by nitrite stress. The transcriptome results show that up-regulation of 718 genes and down-regulation of 1,071 genes after nitrite stress. Most of the differentially expressed genes (DEGs) after nitrite stress were related to physiological processes, including oxidative stress, energy metabolism, and lysosomes. Compared with nitrite stress, there are 911 up-regulated genes and 713 down-regulated genes activated by autophagy, which are related to immune response, specifically involving glutathione metabolism, drug metabolism-cytochrome P450, and metabolism of xenobiotics by cytochrome P450, lysosomes, autophagy, phagosomes, and MAPK signaling pathways. These pathways can reduce oxidative stress and promote the metabolism of harmful substances in the body, in shrimp, thereby improving their survival rates. These findings provide new insights for research aimed at enhancing nitrite tolerance in shrimp.

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Section: Introductionmentioning

confidence: 99%

Transcriptome revealed the role of autophagy in nitrite oxidative stress of Penaeus vannamei

Lin,

Liu,

Chen

et al. 2024

Preprint

Self Cite

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“…vannamei is reared in semi‐intensive and intensive culture systems, which often suffer from a variety of environmental stresses. These stresses, such as ammonia nitrogen (Liang et al, 2016; Liu & Chen, 2004; Wang et al, 2020), nitrite (Tseng & Chen, 2004), hypoxia (Lehmann et al, 2016; Liu et al, 2015), abnormal salinity (Gao et al, 2016; Wang & Chen, 2005) and abnormal pH (Li & Chen, 2008), can trigger oxidative stress, affect the antioxidant capacity and the immune capacity, and lead to disease outbreaks of L . vannamei , restricting the healthy development of shrimp aquaculture.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of sulphoraphane on oxidative damage caused by ammonia in Litopenaeus vannamei

Yang

Huang

Chen

et al. 2021

Aquaculture Research

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To study the effects of supplemental sulphoraphane (SFN) on oxidative damage caused by ammonia in Litopenaeus vannamei, a feeding trial and an ammonia‐N stress trial (20 mg L−1) were performed in succession using a commercial formulation containing four SFN concentrations (0, 10, 30 and 50 mg kg−1). At the end of the feeding trial, the malondialdehyde (MDA) level in the control group was the highest. The activities of superoxide dismutase (SOD) in the muscles in the control group were significantly lower than those of shrimps supplemented with SFN (p < 0.05). The activities of glutathione peroxidase (GPX) in all tissues and the activities of catalase (CAT) in the serum in shrimps supplemented with 30 and 50 mg kg−1 SFN were higher than those in the control group (p < 0.05). The gene expression of SOD and CAT in shrimps supplemented with 10 and 30 mg kg−1 SFN was significantly higher than those in the control group (p < 0.05). After the ammonia‐N stress trial, the highest MDA level was observed in the control group. However, the expression of SOD, GPX, CAT and TRX in the control group was higher than those in shrimps supplemented with 30 and 50 mg kg−1 SFN (p < 0.05). Overall, the results indicated that SFN significantly decreases oxidative damage and improves the antioxidant capacity of L. vannamei and has promising application potential in protecting shrimps against environmental stress.

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“…However, with the expansion of demand for shrimp production, the cultivation scale and the cultivation density are increasing. Shrimp subjects to serious environmental stress, such as ammonia nitrogen, nitrite and unnormal pH, which could increase oxidative stress and the susceptibility of shrimp to pathogens, and lead to the outbreak and epidemic of diseases (Tseng et al, 2004;Li et al, 2008;Liu et al, 2015a;Liang et al, 2016;Wang et al, 2020a). Ammonia nitrogen (ammonia), a common environmental stressor for aquatic animals, originates from excretion of the animals and decomposing organic wastes such as residual feed and feces in the water.…”

Section: Introductionmentioning

confidence: 99%

Dietary resveratrol attenuates oxidative stress in Pacific whiteleg shrimp, Litopenaeus vannamei, in response to ammonia stress

Liu

Zheng

Huang

et al. 2021

Israeli Journal of Aquaculture - Bamidgeh

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To study the effect of resveratrol on the antioxidant system of Litopenaeus vannamei, resveratrol (0, 40, 80, 120 and 180 mg/kg) was added to the feed. After 7 days of culture, 10 tails of L. vannamei were randomly selected and exposure to ammonia stress at the sublethal concentration of ammonia (20 mg/L) for 24h. The results showed that the malondialdehyde (MDA) level in serum of shrimp fed with resveratrol decreased significantly compare to the control group (p< 0.05). After the ammonia stress, the MDA levels in serum in the Res 120 group and the Res 160 group was lower significantly than that in the control group (p< 0.05). The activities of superoxide dismutase (SOD) in the hepatopancreas and the serum, and glutathione peroxidase (GPX) in the serum of shrimps fed with resveratrol showed a decrease trend before the ammonia stress. Conversely, the activities of SOD and the total antioxidant competence (T-AOC) levels in the hepatopancreas in the Res 80 group and Res 120 group were significantly higher than that in the control group after the ammonia stress (p<0.05). Dietary resveratrol decreased oxidative damage, increased antioxidant enzyme activities under the ammonia stress. Our results demonstrated that resveratrol attenuated oxidative stress and improved the resistance to the ammonia stress.

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Tuberous sclerosis complex 1 (PvTSC1) participates in ammonia nitrogen induced oxidative stress in Penaeus vannamei by regulating autophagy

Cited by 9 publications

References 41 publications

Transcriptome revealed the role of autophagy in nitrite oxidative stress of Penaeus vannamei

Transcriptome revealed the role of autophagy in nitrite oxidative stress of Penaeus vannamei

Protective effects of sulphoraphane on oxidative damage caused by ammonia in Litopenaeus vannamei

Dietary resveratrol attenuates oxidative stress in Pacific whiteleg shrimp, Litopenaeus vannamei, in response to ammonia stress

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