TubeSpin satellites: a fast track approach for process development with animal cells using shaking technology

Jesus, Maria J. De; Girard, Philippe; Bourgeois, M.; Baumgartner, G.; Jacko, Barbara; Amstutz, Hanspeter; Wurm, Florian Μ.

doi:10.1016/s1369-703x(03)00180-3

Cited by 149 publications

(83 citation statements)

References 6 publications

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“…These reasons kindled the interest in the use of large scale orbital shaken devices up to thousand liters. [7][8][9] Thus, a large number of researches have been performed on the gas exchange, [10][11][12][13] mixing, [14][15][16][17] and volumetric power consumption 18 of the bioreactors. In some cases, correlations have been proposed in order to quantify the variation of those quantities during scale-up of the culture size.…”

Section: Introductionmentioning

confidence: 99%

Surface wave dynamics in orbital shaken cylindrical containers

et al. 2014

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Be it to aerate a glass of wine before tasting, to accelerate a chemical reaction, or to cultivate cells in suspension, the "swirling" (or orbital shaking) of a container ensures good mixing and gas exchange in an efficient and simple way. Despite being used in a large range of applications this intuitive motion is far from being understood and presents a richness of patterns and behaviors which has not yet been reported. The present research charts the evolution of the waves with the operating parameters identifying a large variety of patterns, ranging from single and multiple crested waves to breaking waves. Free surface and velocity fields measurements are compared to a potential sloshing model, highlighting the existence of various flow regimes. Our research assesses the importance of the modal response of the shaken liquids, laying the foundations for a rigorous mixing optimization of the orbital agitation in its applications. C 2014 AIP Publishing LLC. [http://dx

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Section: Introductionmentioning

confidence: 99%

Surface wave dynamics in orbital shaken cylindrical containers

et al. 2014

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Section: Introductionsupporting

confidence: 58%

“…The tube culture system was developed by De Jesus et al (2004), and this work confirmed the usefulness of this system for CHO cells. The main advantage of 50-mL tube bioreactors is the scaling down of the system, while still providing enough sample volume for further analysis and minimizing evaporation problems (Betts and Baganz, 2006;de Jesus et al, 2004;Jordan and Jenkins, 2007). Tube cultures are able to mimic large scale vessel behaviour, as the results discovered from 50ml tube bioreactors can be fitted into the "real" condition found The aim of this work is to optimize culture conditions for insect cells using high throughput 50-mL tube bioreactors.…”

Section: Introductionsupporting

confidence: 58%

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Metabolomic study of baculovirus infected insect cells to identify metabolite based strategies for improving virus yields

Tran¹

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The baculovirus insect cell system has been used widely for biopesticide applications and for the production of recombinant proteins, vaccines and vectors for gene delivery. To be commercially viable, especially for the low margin applications of biopesticides and veterinary vaccines, the yield of this production system needs to be improved. One of the important approaches is the infection of insect cell cultures at high cell densities in order to maximize volumetric production. However, the cell specific yield gradually reduces with increasing infection cell density (ICD). As a result, the volumetric production can not improve further regardless of efforts to increase the uninfected cell density in batch or fedbatch cultures. The phenomenon known as "the cell density effect" appears not only during the late stage of protein production, but also during the early stage of virus replication and transcription. Discovering the changes in intra and extracellular metabolites between low and high ICDs may reveal evidence for improving yields. This PhD thesis focuses on the development of an intracellular metabolite extraction protocol for infected insect cell cultures and for measuring intra and extracellular metabolites of infected cells of different baculovirus infected insect cell systems.Prior to analyzing the intracellular metabolites of low and high ICDs, an extraction protocol was developed and validated for insect cell cultures, especially infected insect cells. The ice-cold saline quenching solution developed for mammalian cells did not work well for insect cells, especially for infected cultures. Therefore, a modification of the quenching solution was made by an addition of 0.2% Pluronic® F-68 that successfully protected the cell membrane during quenching and washing procedures. In addition, one wash was found to be enough for removal of medium originated metabolites adhering on the surface of cell membranes. The ATP recovery (over the direct extract) of the extraction protocol using ice-cold saline quenching solution plus 0.2% Pluronic® F-68 after one wash was almost 90% for Spodoptera frugiperda (Sf9) cells infected with a recombinant Autographa californica multiple nucleopolyhedrovirus (rAcMNPV) and Helicoverpa zea (HzAM1) cells infected with a wild-type Helicoverpa armigera single nucleopolyhedrovirus (HearNPV) at 24 hours post infection (hpi).The intracellular metabolite patterns and substrate consumption rates of low versus high ICDs was first investigated for the Sf9/rAcMNPV system in Sf900™III medium. A preliminary study was conducted to identify how late post-infection the cell membrane integrity was maintained during the quenching and washing processes. The result showed that the extraction protocol is efficient up to 48 hpi for infected Sf9 cells. Levels of intracellular nucleotides (tri-phosphates), amino acids (AA), and TCA cycle intermediates iii (α-ketoglutarate, pyruvate) during an infection at a low ICD (2×10 6 cells/mL) were shown to be higher than those during an infection at a high...

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“…Suspension-adapted Chinese hamster ovary cells (CHO DG44) were cultivated in ProCHO5 medium (Lonza Verviers SPRL) as previously described (De Jesus et al 2003;Muller et al 2005). …”

Section: Cell Culturementioning

confidence: 99%

Suspension-adapted Chinese hamster ovary-derived cells expressing green fluorescent protein as a screening tool for biomaterials

et al. 2009

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Synthetic biomaterials play an important role in regenerative medicine. To be effective they must support cell attachment and proliferation in addition to being non-toxic and non-immunogenic. We used a suspension-adapted Chinese hamster ovary-derived cell line expressing green fluorescent protein (GFP) to assess cell attachment and growth on synthetic biomaterials by direct measurement of GFP-specific fluorescence. To simplify operations, all cell cultivation steps were performed in orbitallyshaken, disposable containers. Comparative studies between this GFP assay and previously established cell quantification assays demonstrated that this novel approach is suitable for rapid screening of a large number of samples. Furthermore the utility of our assay system was confirmed by evaluation of cell growth on three polyvinylidene fluoride polymer scaffolds that differed in pore diameter and drawing conditions. The data presented here prove the general utility of GFP-expressing cell lines and orbital shaking technology for the screening of biomaterials for tissue engineering applications.

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TubeSpin satellites: a fast track approach for process development with animal cells using shaking technology

Cited by 149 publications

References 6 publications

Surface wave dynamics in orbital shaken cylindrical containers

Surface wave dynamics in orbital shaken cylindrical containers

Metabolomic study of baculovirus infected insect cells to identify metabolite based strategies for improving virus yields

Suspension-adapted Chinese hamster ovary-derived cells expressing green fluorescent protein as a screening tool for biomaterials

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