Tubgcp3 Is Required for Retinal Progenitor Cell Proliferation During Zebrafish Development

Li, Guobao; Jin, Da-Qing; Zhong, Tao

doi:10.3389/fnmol.2019.00126

Cited by 8 publications

(11 citation statements)

References 49 publications

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“…The TUBGCP3 (Tubulin Gamma Complex Associated Protein 3) is an important part of Gamma-tubulin complex, which is necessary for microtubule nucleation at the centrosome. 26 Previous studies have reported that TUBGCP3 plays a vital part in forming the structural constituent of cytoskeleton and microtubule nucleation by interphase microtubule organizing center. 27 It is well established that circRNAs originate from a series of exons.…”

Section: Discussionmentioning

confidence: 99%

<p>Circular RNA circTUBGCP3 Is Up-Regulated and Promotes Cell Proliferation, Migration and Survivability via Sponge mir-30b in Osteosarcoma</p>

Teng

Huang

et al. 2020

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Prevailing evidences have demonstrated that circular RNAs (circRNAs) are closely associated with various stages of carcinogenesis. However, very few studies have delineated the specific mechanism of association between circRNAs and osteosarcoma (OS). It offers a novel insight that circRNAs can be explored as a potential therapeutic strategy for OS. Materials and Methods: In this study, circTUBGCP3 was chosen from the existing reported circRNA microarray data obtained from OS cell lines and normal bone cells. Subsequently, qRT-PCR was performed to evaluate the expression level of circTUBGCP3 in OS samples and cell lines. Functional assays were conducted to estimate the impact of circTUBGCP3 on human OS cells proliferation, vitality, survivability, and migration. Western blot, luciferase reporter and in vivo tumorigenesis assays were performed to analyze the signaling pathways underlying the interaction of circTUBGCP3, miR-30b, and Vimentin. Results: The data indicate that circTUBGCP3 may act as a sponge of miR-30b that further alters the expression of Vimentin, and promotes the proliferation and metastatic properties of OS cells. Conclusion: circTUBGCP3 serves as a tumor promoter in tumorigenesis by increasing the possibilities of OS initiation and proliferation.

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Section: Discussionmentioning

confidence: 99%

<p>Circular RNA circTUBGCP3 Is Up-Regulated and Promotes Cell Proliferation, Migration and Survivability via Sponge mir-30b in Osteosarcoma</p>

Teng

Huang

et al. 2020

OTT

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“…involved with eye morphological alterations(Du et al, 2015;Ergun et al, 2017;Li et al, 2019). Thus, this agnostic analysis of rare nonsense variants successfully identified candidate genes in A/M.…”

mentioning

confidence: 84%

“…and found evidence that each is involved in eye development(Bergman et al, 2012;Du et al, 2015;Ergun, Akay, Ergun, & Percin, 2017;Li, Jin, & Zhong, 2019;Meijer, 1993;Papakostas et al, 2018). For example, LRP5, NUMA1 and TUBGCP3 have been identified to be involved in retinal development and KRT31 to be…”

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confidence: 99%

Exome sequencing identifies genetic variants in anophthalmia and microphthalmia

Li,

Yang,

Wang

et al. 2022

American J of Med Genetics Pt A

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Anophthalmia and microphthalmia (A/M) are rare birth defects affecting up to 2 per 10,000 live births. These conditions are manifested by the absence of an eye or reduced eye volumes within the orbit leading to vision loss. Although clinical case series suggest a strong genetic component in A/M, few systematic investigations have been conducted on potential genetic contributions owing to low population prevalence. To overcome this challenge, we utilized DNA samples and data collected as part of the National Birth Defects Prevention Study (NBDPS). The NBDPS employed multi-center ascertainment of infants affected by A/M. We performed exome sequencing on 67 family trios and identified numerous genes affected by rare deleterious nonsense and missense variants in this cohort, including de novo variants. We identified 9 nonsense changes and 86 missense variants that are absent from the reference human population (Genome Aggregation Database), and we suggest that these are high priority candidate genes for A/M. We also performed literature curation, single cell transcriptome comparisons, and molecular pathway analysis on the candidate genes and performed protein structure modeling to determine the potential pathogenic variant consequences on PAX6 in this disease.

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“…Similar to lethality and abnormality effects study, all of the embryos were divided into four groups for this immunohistochemistry (IHC) analysis [ 59 ]. In this analysis, the protein expressions of DNA damages and apoptosis were investigated.…”

Section: Methodsmentioning

confidence: 99%

Radioprotective Effects of Kelulut Honey in Zebrafish Model

et al. 2021

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Large doses of ionizing radiation can damage human tissues. Therefore, there is a need to investigate the radiation effects as well as identify effective and non-toxic radioprotectors. This study evaluated the radioprotective effects of Kelulut honey (KH) from stingless bee (Trigona sp.) on zebrafish (Danio rerio) embryos. Viable zebrafish embryos at 24 hpf were dechorionated and divided into four groups, namely untreated and non-irradiated, untreated and irradiated, KH pre-treatment and amifostine pre-treatment. The embryos were first treated with KH (8 mg/mL) or amifostine (4 mM) before irradiation at doses of 11 Gy to 20 Gy using gamma ray source, caesium-137 (137Cs). Lethality and abnormality analysis were performed on all of the embryos in the study. Immunohistochemistry assay was also performed using selected proteins, namely γ-H2AX and caspase-3, to investigate DNA damages and incidences of apoptosis. KH was found to reduce coagulation effects at up to 20 Gy in the lethality analysis. The embryos developed combinations of abnormality, namely microphthalmia (M), body curvature and microphthalmia (BM), body curvature with microphthalmia and microcephaly (BMC), microphthalmia and pericardial oedema (MO), pericardial oedema (O), microphthalmia with microcephaly and pericardial oedema (MCO) and all of the abnormalities (AA). There were more abnormalities developed from 24 to 72 h (h) post-irradiation in all groups. At 96 h post-irradiation, KH was identified to reduce body curvature effect in the irradiated embryos (up to 16 Gy). γ-H2AX and caspase-3 intensities in the embryos pre-treated with KH were also found to be lower than the untreated group at gamma irradiation doses of 11 Gy to 20 Gy and 11 Gy to 19 Gy, respectively. KH was proven to increase the survival rate of zebrafish embryos and exhibited protection against organ-specific abnormality. KH was also found to possess cellular protective mechanism by reducing DNA damage and apoptosis proteins expression.

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Tubgcp3 Is Required for Retinal Progenitor Cell Proliferation During Zebrafish Development

Cited by 8 publications

References 49 publications

<p>Circular RNA circTUBGCP3 Is Up-Regulated and Promotes Cell Proliferation, Migration and Survivability via Sponge mir-30b in Osteosarcoma</p>

<p>Circular RNA circTUBGCP3 Is Up-Regulated and Promotes Cell Proliferation, Migration and Survivability via Sponge mir-30b in Osteosarcoma</p>

Exome sequencing identifies genetic variants in anophthalmia and microphthalmia

Radioprotective Effects of Kelulut Honey in Zebrafish Model

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