Tubular invaginations with caveolae and coated pits in the sinus endothelial cells of the rat spleen

Uehara, Kiyoko; Miyoshi, Masayuki

doi:10.1007/s004180050416

Cited by 12 publications

(7 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, TRPC1 was reported to be assembled in signaling complexes associated with caveolin-scaffolding lipid raft domains and to interact with one or more components of the complex, including IP 3 R (Lockwich et al 2000). The sinus endothelial cells have abundant caveolae containing caveolin-1, À2, and À3 (Uehara and Miyoshi 1999b, 1999c. The localization of TRPC1 in caveolae shown in this study is consistent with these results.…”

Section: Discussionsupporting

confidence: 93%

Localization of TRPC1 channel in the sinus endothelial cells of rat spleen

Uehara

2005

Histochem Cell Biol

View full text Add to dashboard Cite

The ultrastructural localization of transient receptor potential C1 (TRPC1) channels in the sinus endothelial cells of rat spleen was examined by confocal laser scanning and electron microscopy. In addition, the localization of the closely associated proteins and channels, VE-cadherin, calreticulin, inositol-1,4,5-trisphosphate receptors type 1 (IP3R1), and ryanodine receptor (RyR), was also examined. Immunofluorescence microscopy of tissue cryosections revealed TRPC1 channels to be localized within the cytoplasm, in the superficial layer of the apical and basal parts of the cells, and in the junctional area of the adjacent endothelial cells. The distribution of Ca2+-storing tubulovesicular structures within endothelial cells was established by using tissue sections treated with osmium ferricyanide. Electron microscopy revealed densely stained tubulovesicular structures closely apposed to the plasma membrane and that occasionally ran closely parallel to the plasma membrane and near the caveolae and junctional apparatus. Immunolocalization analysis at the electron microscopy level using immunogold bound to the secondary antibody confirmed that TRPC1 channels were localized in the plasma membrane, caveolae, and vesicular structures in the subplasmalemmal cytoplasm of sinus endothelial cells. Calreticulin was predominantly localized in endoplasmic reticulum. IP3R1 and RyR, considered to be type 3, were colocalized in endoplasmic reticulum in proximity to the plasma membrane and caveolae. Thus, TRPC1 channels in sinus endothelial cells of the spleen might play an important role in controlling blood cell passage through phenomena including cytoskeletal reorganization, cell retraction, and disassembly of adherens junctions.

show abstract

Section: Discussionsupporting

confidence: 93%

Localization of TRPC1 channel in the sinus endothelial cells of rat spleen

Uehara

2005

Histochem Cell Biol

View full text Add to dashboard Cite

show abstract

“…Furthermore, during the differentiation of primary cultured muscle cells and the development of mouse skeletal muscle in vivo, caveolin-3 was demonstrated to transiently localize in the transverse tubules (TT) with clusters of caveolae (Franzini-Armstrong 1991; Parton et al 1997). We have reported tubular invaginations with clusters of caveolae in sinus endothelial cells (Uehara and Miyoshi 1999b), and in this study we showed that the clusters of caveolae were labeled with anti-caveolin-3 and -1 antibodies. It is questionable whether or not the tubular invaginations with clusters of caveolae in sinus endothelial cells play a role similar to the TT with clusters in the skeletal muscle during its development in Ca 2+ regulation.…”

Section: Discussionmentioning

confidence: 78%

Localization of caveolin-3 in the sinus endothelial cells of the rat spleen

Uehara

Miyoshi

2002

Cell and Tissue Research

Self Cite

View full text Add to dashboard Cite

The localization of caveolins in the sinus endothelial cells of the rat spleen has been demonstrated by confocal laser scanning and electron microscopy. Caveolin-3, a muscle-specific caveolin, was detected by Western blot analysis and immunofluorescence microscopy of isolated sinus endothelial cells and tissue cryosections of the spleen. During the immunofluorescence microscopy of isolated endothelial cells, both caveolin-3 and caveolin-1 were found. In tissue cryosections of the spleen, caveolin-3, as well as caveolin-1 and -2, was present in the contours and cytoplasm of the cells. Immunogold electron microscopy of tissue cryosections revealed caveolin-3, -1, and -2 to be present in caveolae in the apical, lateral, and basal plasma membranes and some vesicular profiles in the cytoplasm of sinus endothelial cells. Furthermore, caveolin-3 was colocalized with caveolin-1 in the same caveolae in the apical, lateral, and basal plasma membranes. Stress fibers and tubulovesicular structures were situated in the vicinity of caveolae labeled with anti-caveolin-3, anti-caveolin-1, and anti-caveolin-2 antibodies. It is speculated that caveolae in sinus endothelial cells play an important role in the constriction of stress fibers.

show abstract

“…ATP-induced [Ca 2+ ](i) increase in sinus endothelial cells is likely to be initiated at the caveolae, the P2Y1 receptor of which is localized in the basal part of sinus endothelial cells. Although numerous caveolae are distributed throughout splenic sinus endothelial cells (Uehara and Miyoshi 1999c), P2Y1 receptor is localized in caveolae in the basal part of endothelial cells. Sinus endothelial cells are a crucial site for controlling blood cell passage through the splenic cord beneath endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

P2Y1, P2Y6, and P2Y12 receptors in rat splenic sinus endothelial cells: an immunohistochemical and ultrastructural study

Uehara¹,

Uehara²

2011

Histochem Cell Biol

View full text Add to dashboard Cite

Localization of three P2X and six P2Y receptors in sinus endothelial cells of the rat spleen was examined by immunofluorescent microscopy, and ultrastructural localization of the detected receptors was examined by immunogold electron microscopy. In immunofluorescent microscopy, labeling for anti-P2Y1, P2Y6, and P2Y12 receptors was detected in endothelial cells, but P2X1, P2X2, P2X4, P2Y2, P2Y4, and P2Y13 receptors was not detected. P2Y1 and P2Y12 receptors were prominently localized in the basal parts of endothelial cells. P2Y6 receptor was not only predominantly localized in the basal parts of endothelial cells, but also in the superficial layer. Triple immunofluorescent staining for a combination of two P2Y receptors and actin filaments showed that P2Y1, P2Y6, and P2Y12 receptors were individually localized in endothelial cells. Phospholipase C-β3, phospholipase C- γ2, and inositol-1,4,5-trisphosphate receptors, related to the release of the intracellular Ca(2+) from the endoplasmic reticulum, were also predominantly localized in the basal parts of endothelial cells. In immunogold electron microscopy, labeling for P2Y1, P2Y6, and P2Y12 receptors were predominantly localized in the basal part of endothelial cells and, in addition, in the junctional membrane, basal plasma membrane, and caveolae in the basal part of endothelial cells. Labeling for phospholipase C-β3 and phospholipase C-γ2 was dominantly localized in the basal parts and in close proximity to the plasma membranes of endothelial cells. The possible functional roles of these P2Y receptors in splenic sinus endothelial cells are discussed.

show abstract

Tubular invaginations with caveolae and coated pits in the sinus endothelial cells of the rat spleen

Cited by 12 publications

References 23 publications

Localization of TRPC1 channel in the sinus endothelial cells of rat spleen

Localization of TRPC1 channel in the sinus endothelial cells of rat spleen

Localization of caveolin-3 in the sinus endothelial cells of the rat spleen

P2Y1, P2Y6, and P2Y12 receptors in rat splenic sinus endothelial cells: an immunohistochemical and ultrastructural study

Contact Info

Product

Resources

About