Tubulation of liposomes via the interaction of supramolecular nanofibers

Abstract

We achieved tubulation of self-assembled lipid membranes, liposomes, via the interaction of supramolecular nanofibers, porphyrin J-aggregates. This structural change was reversible, and the deformation of the porphyrin J-aggregates caused reconstruction of the liposomes from the tubes. We discussed the tubulation mechanism and calculated the force provided by porphyrin J-aggregates for tubulation.

Novel Aggregation-Enhanced PEC Photosensitizer Based on Electrostatic Linkage of Ionic Liquid with Protoporphyrin IX for Ultrasensitive Detection of Molt-4 Cells

“…Clearly, there emerges a classical Soret peak at 363 nm for the PPIX aggregates (curve a), combined with a slight shoulder peak centered at 475 nm. After combination of the PPIX with [BMIm] + (curve b), a sharp peak appears at 378 nm without any shoulder peak, which displays orderly organization of the PPIX molecule in the presence of [BMIm] + , that is the “rigid” structure. , Further, the red-shifts in the absorption bands are instructive to the conjugated aromatic heterocyclic structure and disorderly stacking . According to the AIE theory, such a structure can obviously eliminate intramolecular movement and enhance photoelectron excitation efficiency, which is essential for the PEC reaction of organic molecular.…”

Mechanisms of Motor-Independent Membrane Remodeling Driven by Dynamic Microtubules

“…To conclude, our data show that the three different mechanisms of MT-driven membrane tube extension observed for the ER in cells can be supported by the formation of transient bonds between MTs and membranes and that a biased self-spreading of the lipid bilayer on MTs can account both for the sliding and TAC mechanisms. Whereas previous work has provided the proof of principle for membrane spreading on fibers [33,53], these studies did not aim to reconstitute molecular mechanisms that are operational in the cell. In contrast, the system used here recapitulated the dynamics and affinities found in naturally occurring MT-binding proteins and dynamic MTs.…”

Adsorption of tetrakis(4-sulfophenyl)porphyrin onto liposomal surfaces composed of neutral diacylphosphatidylcholine and release by cyclodextrin

“…The red shi was not because of the formation of onedimensional self-assembled structures because: (i) 3 cannot form self-aggregates under neutral conditions because protonation of 3 is essential for self-association, and (ii) the shi value is too small for self-aggregates because the Soret band of the porphyrin J-aggregates displayed a signicant red shi to 491 nm. [15][16][17][18] If sulfo groups of 3 interact with ammonio groups of 1 on the liposomal surface, the addition of cationic lipids in the liposome should facilitate the formation of strong interactions between anionic 3 and the liposomal surface via electrostatic interactions. [19][20][21] Therefore, a cationic lipid (2) was mixed with lipid 1 in the liposomes {[1] : [2] ¼ 7 : 3 (mol mol À1 )}.…”

“…1) interacts with the liposomal surface by formation of onedimensional self-assembled structures (J-aggregates) under acidic conditions. 15,16 In contrast, neutralization of the solution deformed the porphyrin J-aggregates, leading to release of 3 from the liposomal surface. 15,16 These interactions are important not only for development of novel functional materials by using liposomes, but also for internalization into cells by endocytosis as the rst step of intracellular uptake.…”

“…15,16 In contrast, neutralization of the solution deformed the porphyrin J-aggregates, leading to release of 3 from the liposomal surface. 15,16 These interactions are important not only for development of novel functional materials by using liposomes, but also for internalization into cells by endocytosis as the rst step of intracellular uptake. In this report, 3 was found to interact with liposomal surfaces composed of neutral lipids at high liposome concentrations without the formation of porphyrin J-aggregates (Scheme 1A).…”