Tubulin acetylation accompanies autophagy development induced by different abiotic stimuli in Arabidopsis thaliana

Abstract

Microtubules (MTs) play an important role in the regulation of autophagy development in yeast and animal as well as in plant cells. MTs participate in maturation and traffic of autophagosomes through their dynamic state changes and post-translational modifications of tubulin, namely acetylation. We subjected Arabidopsis thaliana seedlings to metabolic-, salt-, osmotic stresses as well as irradiation of ultraviolet B and investigated the involvement of plant MTs in the development of stress-induced autophagy via tubulin acetylation. For this purpose Arabidopsis thaliana line expressing autophagy-related protein 8 h (atg8h)-GFP was generated to investigate autophagy, applying the level of free GFP as an indicator of autophagy development. Using autophagosome confocal imaging and Western blot analysis of Atg8 post-translational lipidation and synchronous GFP release it was shown that all examined stressful stimuli led to pronounced development of autophagy, particularly in different root tissues. Moreover, autophagy development was accompanied by α-tubulin acetylation under all stressful conditions. Presented data indicate the possible role of the post-translational acetylation of α-tubulin in the mediation of plant stress-induced autophagy.

A Biostimulant Obtained from the Seaweed Ascophyllum nodosum Protects Arabidopsis thaliana from Severe Oxidative Stress

“…Overexpression of ATG8 (different genes including ATG8E) in Arabidopsis leads to an increase in the number of autophagosomes concomitant with an activated autophagy [84]. Similarly, experimental evidence was obtained to indicate that elevated expression of ATG8H increases autophagy during abiotic stress conditions [85]. Expression of both, ATG8E and ATG8H is repressed by SF (Table 1).…”

Autophagy development as an adaptive response to microgravity conditions in Arabidopsis thaliana

“…У той час, як реалізація процесу аутофагії у клітинах тварин є досить детально вивченою, зокрема, було з'ясовано роль аутофагії в онтогенезі клітин, їх відповіді на вплив стресових чинників та за формування вродженого імунітету, залучення аутофагії до реалізації нейродегенеративних та патологічних процесів [6][7][8], молекулярні механізми процесів аутофагії у рослин потребують більш глибоко дослідження. Так, зокрема, раніше нами було продемонстровано вплив ряду стресових абіотичних факторів (голодування, осмотичний і сольовий стресс та УФопромінення) на індукцію та розвиток аутофагії у проростках Arabidopsis thaliana [9].…”

A Journey Through Plant Cytoskeleton: Hot Spots in Signaling and Functioning

“…MTs participate in maturation and traffic of autophagosomes through their dynamic state changes and post‐translational modifications of tubulin, namely acetylation (Xie et al, ), but the role of this modification was not investigated yet during development of plant autophagy. Olenieva et al () used A. thaliana seedlings subjected to metabolic, salt, osmotic stresses as well as irradiation of UVB for investigation the involvement of plant MTs in the development of stress‐induced autophagy via tubulin acetylation. Using A. thaliana line expressing Atg8h‐GFP as marker of autophagosome formation (Ryabovol and Minibayeva, ; Kellner et al, ) it was demonstrated that all examined stressful stimuli led to pronounced development of autophagy, particularly in different root tissues.…”