Tubulin and FtsZ structures: functional and therapeutic implications

Desai, Arshad; Mitchison, Timothy J.

doi:10.1002/(sici)1521-1878(199807)20:7<523::aid-bies1>3.0.co;2-l

Cited by 21 publications

(18 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is well known that microtubules (MT) are formed in vitro by noncovalent polymerization of tubulin dimers (23,(32)(33)(34)(35). Formation of MT from tubulin solution can be followed by observing an increase in absorbance at 340 nm in the presence of GTP and tau (23,32,34,35).…”

Section: Resultsmentioning

confidence: 99%

Tau Phosphorylation at Serine 396 and Serine 404 by Human Recombinant Tau Protein Kinase II Inhibits Tau's Ability to Promote Microtubule Assembly

Evans¹,

Rank²,

Bhattacharya³

et al. 2000

Journal of Biological Chemistry

133

126

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Tau Phosphorylation at Serine 396 and Serine 404 by Human Recombinant Tau Protein Kinase II Inhibits Tau's Ability to Promote Microtubule Assembly

Evans¹,

Rank²,

Bhattacharya³

et al. 2000

Journal of Biological Chemistry

133

126

View full text Add to dashboard Cite

“…To assess their relative activity in the Thetis UI and LI, transcripts for two structural proteins were used as indicators. Actin and tubulin proteins from eukaryotes have only weak amino acid similarity compared with the prokaryotic cytoskeletal proteins (Desai and Mitchison, 1998). The abundances of transcripts were more than four times higher in the UI compared with the control site, whereas there was a significant drop of the abundances in the LI (Supplementary Figure S5).…”

Section: Eukaryotic Signaturesmentioning

confidence: 99%

Unveiling microbial activities along the halocline of Thetis, a deep-sea hypersaline anoxic basin

et al. 2014

View full text Add to dashboard Cite

Deep-sea hypersaline anoxic basins (DHABs) in the Eastern Mediterranean Sea are considered some of the most hostile environments on Earth. Little is known about the biochemical adaptations of microorganisms living in these habitats. This first metatranscriptome analysis of DHAB samples provides significant insights into shifts in metabolic activities of microorganisms as physicochemical conditions change from deep Mediterranean sea water to brine. The analysis of Thetis DHAB interface indicates that sulfate reduction occurs in both the upper (7.0-16.3% salinity) and lower (21.4-27.6%) halocline, but that expression of dissimilatory sulfate reductase is reduced in the more hypersaline lower halocline. High dark-carbon assimilation rates in the upper interface coincided with high abundance of transcripts for ribulose 1,5-bisphosphate carboxylase affiliated to sulfuroxidizing bacteria. In the lower interface, increased expression of genes associated with methane metabolism and osmoregulation is noted. In addition, in this layer, nitrogenase transcripts affiliated to uncultivated putative methanotrophic archaea were detected, implying nitrogen fixation in this anoxic habitat, and providing evidence of linked carbon, nitrogen and sulfur cycles.

show abstract

“…Albendazole and thiabendazole are known inhibitors of tubulin polymerization, and the only known prokaryotic mechanism analogous to tubulin polymerization is FtsZ assembly (Desai & Mitchison, 1998;Downing, 2000). Moreover, the three-dimensional structures of bacterial FtsZ orthologues show striking similarity to the structure of aand b-tubulin and both FtsZ and tubulin possess GTPase activity Nogales et al, 1998).…”

Section: Effect Of Ftsz Inhibition On Bacterial Viability and Cell Momentioning

confidence: 99%

Identification of cell cycle regulators in Mycobacterium tuberculosis by inhibition of septum formation and global transcriptional analysis

2006

View full text Add to dashboard Cite

In Mycobacterium tuberculosis the mechanism of septum formation and regulation of cell division remains undefined. In other bacterial species FtsZ polymerization and septum formation are influenced through protein interactions in addition to transcriptional regulation, and the combination of these provides tight regulation of this process. However, homologues of proteins known to affect FtsZ assembly have not been identified and substantiated in M. tuberculosis. This suggests that M. tuberculosis may possess unique processes for regulation of septum formation. To begin to address this poorly understood aspect of M. tuberculosis physiology, FtsZ inhibitors were used to block cell division and the effects on bacterial morphology and the transcriptional response were examined. Inhibition of septum formation prevented cell division and led to bacterial filamentation. Microarray-based transcriptional profiling allowed the evaluation of multiple metabolic processes in response to inhibition of septum formation and when coupled with bioinformatics provided a means to identify regulatory elements and other gene products that probably influence septum formation. INTRODUCTIONCell division at the molecular level has been well studied in a number of bacteria and it is recognized that septum formation is the first committed step of this event (Harry et al., 1999). In general, inhibition of bacterial division at the point of septum formation is characterized by normal chromosome replication and segregation that is not followed by cellular division leading to a filamentous phenotype . Septum formation is dependent on the assembly of FtsZ into the contractile Z-ring at the site of division (Den Blaauwen et al., 1999;Romberg & Levin, 2003) and additional proteins recruited to the septum site in a sequential fashion, resulting in constriction and cell division. In spite of this biochemical information, the current understanding of regulatory elements and the precise nature of the signals driving the coordination of cell division with other cell cycle processes is largely unknown, especially in Mycobacterium tuberculosis.Many of the proteins involved in cell division, such as FtsZ, are conserved across taxa, while others appear limited to one or a few bacterial groups Margolin, 2000). These include proteins known to interact with FtsZ and that modulate Z-ring assembly Romberg & Levin, 2003). The Z-ring is highly dynamic, and proper assembly of this structure and cell division are strongly influenced by direct interactions of FtsZ with ZipA, ZapA, FtsA, MinD, EzrA and SulA (Harry et al., 1999;Harry, 2001;Migocki et al., 2004;Romberg & Levin, 2003). These factors together with transcriptional regulators form a regulatory network that orchestrates the spatial and temporal control of cell division with other cell cycle processes. However, the underlying molecular mechanisms are only partially understood in model organisms and remain largely unknown for many unrelated organisms.Analysis of the M. tuberculosis genome sequence revealed t...

show abstract

Tubulin and FtsZ structures: functional and therapeutic implications

Cited by 21 publications

References 31 publications

Tau Phosphorylation at Serine 396 and Serine 404 by Human Recombinant Tau Protein Kinase II Inhibits Tau's Ability to Promote Microtubule Assembly

Tau Phosphorylation at Serine 396 and Serine 404 by Human Recombinant Tau Protein Kinase II Inhibits Tau's Ability to Promote Microtubule Assembly

Unveiling microbial activities along the halocline of Thetis, a deep-sea hypersaline anoxic basin

Identification of cell cycle regulators in Mycobacterium tuberculosis by inhibition of septum formation and global transcriptional analysis

Contact Info

Product

Resources

About