Tubulin Beta‐3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study

Abstract

Skin aging is a complex process, and a lot of efforts have been made to identify new and specific targets that could help to diagnose, prevent, and treat skin aging. Several studies concerning skin aging have analyzed the changes in gene expression, and very few investigations have been performed at the protein level. Moreover, none of these proteomic studies has used a global quantitative labeled proteomic offgel approach that allows a more accurate description of aging phenotype. We applied such an approach on human primary keratinocytes obtained from sun-nonexposed skin biopsies of young and elderly women. A total of 517 unique proteins were identified, and 58 proteins were significantly differentially expressed with 40 that were downregulated and 18 upregulated with aging. Gene ontology and pathway analysis performed on these 58 putative biomarkers of skin aging evidenced that these dysregulated proteins were mostly involved in metabolism and cellular processes such as cell cycle and signaling pathways. Change of expression of tubulin beta-3 chain was confirmed by western blot on samples originated from several donors. Thus, this study suggested the tubulin beta-3 chain has a promising biomarker in skin aging.

Proteomic profiling of senescent human diploid fibroblasts treated with gamma-tocotrienol

“…Microtubules are mainly consisted of α- and β-tubulin heterodimers [ 27 ]. Protein level of tubulin beta-3 chain is higher in keratinocyte cells established from elderly donors than young donors, indicates that it may be a biomarker for skin aging [ 28 ]. In this study, TUBB2A protein expression was upregulated in senescent cells compared to young cells.…”

A large scale proteome analysis of the gefitinib primary resistance overcome by KDAC inhibition in KRAS mutated adenocarcinoma cells overexpressing amphiregulin

“…A quantitative map of the proteome of mutant KRAS cells treated with KDAC inhibitors and/or gefitinib is a key step to gain a better understanding of the role of proteins in EGFR-TKI gefitinib resistance. As it is currently known that the treatment of gefitinib alone does not induce apoptosis in KRAS lung adenocarcinoma, in contrast to the combination of gefitinib with KDAC inhibitors [10][9] [11] (a view confirmed in our investigation in Figure S1), we performed our quantitative proteomic analysis based on iTRAQ labeling, as previously described, [18] on H358 cells treated with gefitinib alone or combined with KDAC inhibitors (NAM and TSA). Because proteins may have different roles according to their cellular localization (e.g.…”

“…These steps were performed as described previously. [17] , [18] Briefly, peptides were loaded on the 3100…”

Photoprotection of Cerium Oxide Nanoparticles against UVA radiation-induced Senescence of Human Skin Fibroblasts due to their Antioxidant Properties

“…Skin ageing is caused by environmental aggressors and the passage of time, and is one of the most common dermatologic concerns 1,2 . It is classified into two types—“intrinsic ageing” (attributed to the influence of genes and hormones) and “extrinsic ageing” (induced by environmental factors such as cigarette smoking, poor nutrition and solar radiation) 3,4 .…”