Tubulin is actively exported from the nucleus through the Exportin1/CRM1 pathway

Schwarzerová, Kateřina; Bellinvia, Erica; Martinek, Jan; Sikorová, Lenka; Dostal, V. A.; Libusová, Lenka; Bokvaj, Pavel; Fischer, Lukáš; Schmit, Anne‐Catherine; Nick, Peter

doi:10.1038/s41598-019-42056-6

Cited by 28 publications

(25 citation statements)

References 51 publications

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“…As a MTOC docking protein, CRM1 facilitates microtubule nucleation at the NPC periphery [70]. Several cell-death-related processes, such as autophagy and apoptosis, which are activated under stress, rely on the microtubule system [71,72]. Still, the biological implications of the nuclear retention of MTOC-related proteins, which has been reported in cancer cells, have remained unclear [73,74].…”

Section: Discussionmentioning

confidence: 99%

Disclosing the Interactome of Leukemogenic NUP98-HOXA9 and SET-NUP214 Fusion Proteins Using a Proteomic Approach

Mendes

Jühlen

Bousbata

et al. 2020

Cells

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The interaction of oncogenes with cellular proteins is a major determinant of cellular transformation. The NUP98-HOXA9 and SET-NUP214 chimeras result from recurrent chromosomal translocations in acute leukemia. Functionally, the two fusion proteins inhibit nuclear export and interact with epigenetic regulators. The full interactome of NUP98-HOXA9 and SET-NUP214 is currently unknown. We used proximity-dependent biotin identification (BioID) to study the landscape of the NUP98-HOXA9 and SET-NUP214 environments. Our results suggest that both fusion proteins interact with major regulators of RNA processing, with translation-associated proteins, and that both chimeras perturb the transcriptional program of the tumor suppressor p53. Other cellular processes appear to be distinctively affected by the particular fusion protein. NUP98-HOXA9 likely perturbs Wnt, MAPK, and estrogen receptor (ER) signaling pathways, as well as the cytoskeleton, the latter likely due to its interaction with the nuclear export receptor CRM1. Conversely, mitochondrial proteins and metabolic regulators are significantly overrepresented in the SET-NUP214 proximal interactome. Our study provides new clues on the mechanistic actions of nucleoporin fusion proteins and might be of particular relevance in the search for new druggable targets for the treatment of nucleoporin-related leukemia.

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Section: Discussionmentioning

confidence: 99%

Disclosing the Interactome of Leukemogenic NUP98-HOXA9 and SET-NUP214 Fusion Proteins Using a Proteomic Approach

Mendes

Jühlen

Bousbata

et al. 2020

Cells

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“…We used cytoplasmic nuclear separation technology to detect YAP expression in the nucleus. As tubulin protein expression has been reported in the nucleus ( Schwarzerová et al, 2019 ), we switched to a glycolysis-related enzyme, enolase 1, as a cytoplasmic protein marker. The expression of enolase 1 is not affected by serine deprivation ( Supplementary Figure 4A ).…”

Section: Resultsmentioning

confidence: 99%

Serine Metabolism Regulates YAP Activity Through USP7 in Colon Cancer

Zhao

et al. 2021

Front. Cell Dev. Biol.

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Metabolic reprogramming is a vital factor in the development of many types of cancer, including colon cancer. Serine metabolic reprogramming is a major feature of tumor metabolism. Yes-associated protein (YAP) participates in organ size control and tumorigenesis. However, the relationship between YAP and serine metabolism in colon cancer is unclear. In this study, RNA sequencing and metabolomics analyses indicated significant enrichment of the glycine, serine, and threonine metabolism pathways in serine starvation–resistant cells. Short-term serine deficiency inhibited YAP activation, whereas a prolonged response dephosphorylated YAP and promoted its activity. Mechanistically, USP7 increases YAP stability under increased serine conditions by regulating deubiquitination. Verteporfin (VP) effectively inhibited the proliferation of colon cancer cells and organoids and could even modulate serine metabolism by inhibiting USP7 expression. Clinically, YAP was significantly activated in colon tumor tissues and positively correlated with the expression of phosphoglycerate dehydrogenase (PHGDH) and USP7. Generally, our study uncovered the mechanism by which serine metabolism regulates YAP via USP7 and identified the crucial role of YAP in the regulation of cell proliferation and tumor growth; thus, VP may be a new treatment for colon cancer.

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“…The tubulin proteins belong to microtubule proteins, which play an important role in the synthesis of the cytoskeleton (He et al 2008). Microtubules of all eukaryotic cells are formed by α-and β-tubulin heterodimers (Schwarzerová et al 2019). Microtubules are core element of the cytoskeleton and act a pivotal function in cellular migration, mitosis, mechanical stress, cell polarity, intracellular transport, cell division, and cell morphogenesis (He et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Identification and Expression Analysis of Tubulin Gene Family in Upland Cotton

Chen

Zhao

et al. 2020

Preprint

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Background: Upland cotton (Gossypium hirsutum) is one of the important cash crops. Therefore, improving fiber quality of upland cotton, is one of the current important research directions. Cotton fibers are single-celled extensions of the seed epidermis, which is important pattern material of research cytoskeleton. Tubulin genes act an important role in the synthesis of the microtubules (MT), which is core element of the cytoskeleton. Some tubulin genes in cotton ovule and fiber development are were reported, while the systematic study of its family genes has not yet in cotton. Therefore, identification and expression analyses of G. hirsutum tubulin genes provide pivotal targets for molecular manipulation in cotton breeding.Result: In this study, we investigated all tubulin genes from seven varies plant species, and identified 98 tubulin genes in G. hirsutum. Phylogenetic analysis showed that tubulin family genes were classified into three subfamilies. The protein motifs and gene structure of α, β-tubulin genes are more conservative comparing with γ-tubulin genes. Most tubulin genes are located at the proximate ends of the chromosomes. Spatiotemporal expression pattern by transcriptome and qRT-PCR analysis revealed that 12 α-tubulin and 7 β-tubulin genes are specific expression in different stages of fiber development. However, Gh.A03G027200, Gh.D03G169300 and Gh.A11G258900 had clear express pattern at distinct stages of ﬁber development in J02508 and ZRI015.Conclusion: In this study, evolutionary analysis showed that the tubulin genes were divided into 3 clades. The genetic structures and molecular functions were highly conserved in different plants. Three candidate genes of Gh.A03G027200, Gh.D03G169300 and Gh.A11G258900 may play key roles during length and strength in fiber development with the expression analysis at different organizations.

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Tubulin is actively exported from the nucleus through the Exportin1/CRM1 pathway

Cited by 28 publications

References 51 publications

Disclosing the Interactome of Leukemogenic NUP98-HOXA9 and SET-NUP214 Fusion Proteins Using a Proteomic Approach

Disclosing the Interactome of Leukemogenic NUP98-HOXA9 and SET-NUP214 Fusion Proteins Using a Proteomic Approach

Serine Metabolism Regulates YAP Activity Through USP7 in Colon Cancer

Identification and Expression Analysis of Tubulin Gene Family in Upland Cotton

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