Tubuloids derived from human adult kidney and urine for personalized disease modeling

Schutgens, Frans; Rookmaaker, Maarten B.; Margaritis, Thanasis; Rios, Anne C.; Ammerlaan, Carola; Jansen, Jitske; Gijzen, Linda; Vormann, Marianne K.; Vonk, Annelotte M.; Viveen, Marco C.; Yengej, Fjodor Yousef; Derakhshan, Sepide; Groot, K.M. de Winter-de; Artegiani, Benedetta; Boxtel, Ruben van; Cuppen, Edwin; Hendrickx, Antoni P. A.; Heuvel‐Eibrink, Marry M. van den; Heitzer, Ellen; Lanz, Henriëtte L.; Beekman, Jeffrey M.; Murk, Jean-Luc; Masereeuw, Rosalinde; Holstege, Frank C.P.; Drost, Jarno; Verhaar, Marianne C.; Clevers, Hans

doi:10.1038/s41587-019-0048-8

Cited by 356 publications

(395 citation statements)

References 81 publications

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“…By providing R-spondin-1, epidermal growth factor (EGF) and Noggin, and embedment of the cells in an extracellular matrix-providing basement membranes extract, the Lgr5-expressing stem cells received the signalling cues necessary to self-renew, proliferate and form differentiated offspring, resembling the intestinal epithelium (Sato et al, 2009). Since then, organoid cultures have been established for a variety of human tissues, including lung (Hild & Jaffe, 2016;Tan et al, 2017;Sachs et al, 2019), colon (Sato et al, 2011), stomach (Bartfeld et al, 2015), liver , pancreas (Boj et al, 2015), prostate (Chua et al, 2014;Karthaus et al, 2014), kidney (Jun et al, 2018;Schutgens et al, 2019) and fallopian tube (Kessler et al, 2015). Moreover, organoid culture protocols have been established for patient-derived tumour tissue as well.…”

Section: Pdto Modelsmentioning

confidence: 99%

“…(Toolan, ; Taetle et al , ; Fu et al , ; Beckhove et al , ; Fichtner et al , ; Shultz et al , ; Wang et al , ; Cutz et al , ; Sato et al , , ; Hennessey et al , ; Gao et al , ; Karthaus et al , ; Boj et al , ; Lee et al , ; Li et al , , ; Sachs et al , ; Yan et al , ; Kopper et al , ; Schutgens et al , ).…”

Section: Introductionunclassified

“…Since then, organoid cultures have been established for a variety of human tissues, including lung (Hild & Jaffe, ; Tan et al , ; Sachs et al , ), colon (Sato et al , ), stomach (Bartfeld et al , ), liver (Huch et al , ), pancreas (Boj et al , ), prostate (Chua et al , ; Karthaus et al , ), kidney (Jun et al , ; Schutgens et al , ) and fallopian tube (Kessler et al , ). Moreover, organoid culture protocols have been established for patient‐derived tumour tissue as well.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, organoid culture protocols have been established for patient‐derived tumour tissue as well. Human tumour organoids have been generated from colon (Sato et al , ; van de Wetering et al , ), pancreas (Boj et al , ; Huang et al , ), prostate (Gao et al , ; Drost et al , ), breast (Sachs et al , ), gastric (Nanki et al , ; Yan et al , ), lung (Sachs et al , ), oesophageal (Li et al , ), bladder (Lee et al , ; Mullenders et al , ), ovarian (Kopper et al , ), kidney (Schutgens et al , ) and liver (Broutier et al , ; Li et al , ) tumour tissue (Fig ). An important feature of a number of these PDTOs is that they genetically and phenotypically mirror the tumour epithelium, including its intra‐tumour heterogeneity (Huang et al , ; van de Wetering et al , ; Nanki et al , ; Sachs et al , ; Yan et al , ).…”

Section: Introductionmentioning

confidence: 99%

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Xenograft and organoid model systems in cancer research

et al. 2019

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Patient‐derived tumour xenografts and tumour organoids have become important preclinical model systems for cancer research. Both models maintain key features from their parental tumours, such as genetic and phenotypic heterogeneity, which allows them to be used for a wide spectrum of applications. In contrast to patient‐derived xenografts, organoids can be established and expanded with high efficiency from primary patient material. On the other hand, xenografts retain tumour–stroma interactions, which are known to contribute to tumorigenesis. In this review, we discuss recent advances in patient‐derived tumour xenograft and tumour organoid model systems and compare their promises and challenges as preclinical models in cancer research.

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Section: Pdto Modelsmentioning

confidence: 99%

Section: Introductionunclassified

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Xenograft and organoid model systems in cancer research

et al. 2019

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“…Currently, organoids are used as models of different pathologies, including infectious diseases caused by viruses, bacteria, and protozoans [3][4][5]. Viral infection studies with organoid systems have included: norovirus, rotavirus, enteric adenovirus, and coronavirus invasion of intestinal organoids [3][4][5][6]; herpes simplex virus 1 [23] and cytomegalovirus [24] infection of cerebral organoids; Zika virus infection of cerebral [4,5] and human testicular organoids [25]; human airway organoids to model pathology and assess infectivity of emerging influenza [26], parainfluenza [27] and respiratory syncytial viruses [28]; BK virus infection in human kidney tubuloids [29]; and human liver organoids to study infection with hepatitis B and its related tumorigenesis [30].…”

Section: Organoids: What Whence and Where To Infection Biologymentioning

confidence: 99%

Organoids – New Models for Host–Helminth Interactions

Duque-Correa

Maizels

Grencis

et al. 2020

Trends in Parasitology

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Organoids are multicellular culture systems that replicate tissue architecture and function, and are increasingly used as models of viral, bacterial, and protozoan infections. Organoids have great potential to improve our current understanding of helminth interactions with their hosts and to replace or reduce the dependence on using animal models. In this review, we discuss the applicability of this technology to helminth infection research, including strategies of co-culture of helminths or their products with organoids and the challenges, advantages, and drawbacks of the use of organoids for these studies. We also explore how complementing organoid systems with other cell types and components may allow more complex models to be generated in the future to further investigate helminth-host interactions.

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Reconfigurable Microphysiological Systems for Modeling Innervation and Multitissue Interactions

et al. 2020

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Tissue‐engineered models continue to experience challenges in delivering structural specificity, nutrient delivery, and heterogenous cellular components, especially for organ‐systems that require functional inputs/outputs and have high metabolic requirements, such as the heart. While soft lithography has provided a means to recapitulate complex architectures in the dish, it is plagued with a number of prohibitive shortcomings. Here, concepts from microfluidics, tissue engineering, and layer‐by‐layer fabrication are applied to develop reconfigurable, inexpensive microphysiological systems that facilitate discrete, 3D cell compartmentalization, and improved nutrient transport. This fabrication technique includes the use of the meniscus pinning effect, photocrosslinkable hydrogels, and a commercially available laser engraver to cut flow paths. The approach is low cost and robust in capabilities to design complex, multilayered systems with the inclusion of instrumentation for real‐time manipulation or measures of cell function. In a demonstration of the technology, the hierarchal 3D microenvironment of the cardiac sympathetic nervous system is replicated. Beat rate and neurite ingrowth are assessed on‐chip and quantification demonstrates that sympathetic‐cardiac coculture increases spontaneous beat rate, while drug‐induced increases in beating lead to greater sympathetic innervation. Importantly, these methods may be applied to other organ‐systems and have promise for future applications in drug screening, discovery, and personal medicine.

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Tubuloids derived from human adult kidney and urine for personalized disease modeling

Cited by 356 publications

References 81 publications

Xenograft and organoid model systems in cancer research

Xenograft and organoid model systems in cancer research

Organoids – New Models for Host–Helminth Interactions

Reconfigurable Microphysiological Systems for Modeling Innervation and Multitissue Interactions

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