Tubulointerstitial Nephritis Antigen-Like 1 Is Expressed in the Uterus and Binds with Integrins in Decidualized Endometrium During Postimplantation in Mice1

Tajiri, Yumiko; Igarashi, Tadashi; Li, Dan; Mukai, Kuniaki; Suematsu, Makoto; Fukui, Emiko; Yoshizawa, Midori; Matsumoto, Hiromichi

doi:10.1095/biolreprod.109.080028

Cited by 26 publications

(33 citation statements)

References 27 publications

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“…Probes had specific activities of about 2 × 10 9 dpm/μg. Uterine tissues were snap-frozen using freezing solution (FREEZER, HOZAN, Osaka, Japan) as described previously [21,22]. Frozen sections (10 μm) were prepared with a cryostat, mounted onto poly-L-lysine-coated slides and fixed in cold 4% paraformaldehyde in phosphate-buffered saline.…”

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confidence: 99%

Differential Expression of the Motin Family in the Peri-implantation Mouse Uterus and Their Hormonal Regulation

Matsumoto

Fukui

Yoshizawa

et al. 2012

Journal of Reproduction and Development

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Abstract. Increased vascular permeability and angiogenesis are hallmarks of the implantation process in the uterus. Angiomotin (Amot), which is a vascular angiogenesis-related protein, belongs to the motin family. There are two other members of the motin family, angiomotin-like 1 and 2 (Amotl1 and 2), which are also thought to be involved with angiogenesis. In the present study, the distribution of motin mRNAs in the mouse uterus during the peri-implantation period was investigated by in situ hybridization. Amot and Amotl1 were expressed in the stromal cells on days 3 and 4; expressions of Amotl2 during the same period were low. During the postimplantation period, Amot and Amotl1 were expressed in secondary decidual cells, while Amotl2 expression fell to an undetectable level. We also examined hormonal regulation of motin expression by steroid hormone treatment in ovariectomized mice. We found that expression of Amot was induced by P 4 in stromal cells. Additionally, Amotl1 expression was upregulated by both P 4 and estrogen (E 2 ) in stromal cells, whereas E 2 increased this gene expression for only a limited time; after 12 h, expression dissipated. In contrast, P 4 regulated the expression of Amotl2 in stromal cells, while E 2 regulated its expression in luminal epithelium cells. Our results demonstrated that Amot, Amotl1, and Amotl2 were differentially expressed in uterine cells during the peri-implantation period, and that their expressions were differentially regulated by P 4 and E 2 . Key words: Angiomotin, Amotl1, Amotl2, Steroid hormone, Uterus (J. Reprod. Dev. 58: [649][650][651][652][653] 2012) S ynchronization of embryonic development and uterine differentiation to its receptive state is necessary to establish a successful pregnancy. Uterine receptivity for implantation lasts for a limited period of time in mice [1][2][3]. In this state, the uterine environment is able to support blastocyst growth, attachment and the subsequent events of implantation. The major hormones that specify uterine receptivity are the ovarian steroids progesterone (P 4 ) and estrogen. The uterus is pre-receptive on day 3 of pregnancy (day 1 = vaginal plug) and enters the receptive phase on day 4 under the influence of rising P 4 and a small amount of estrogen secretion on the morning of day 4 [4,5]. Because P 4 and estrogen primarily target the uterus to regulate its functions during the peri-implantation period, it is thought that these hormones also modulate angiogenesis and decidualization in the uterus during pregnancy [6][7][8].Angiogenesis physiologically occurs in the uterus during pregnancy [9,10]. Increased uterine vascular permeability and angiogenesis are two hallmarks of the implantation process. The proangiogenic factor Vegf (vascular endothelial growth factor) and its receptor Flk1 (vascular endothelial growth factor receptor-2) are primarily important for uterine vascular permeability and angiogenesis prior to and during the attachment phase of the implantation process [6,9]. Meanwhile, Vegf in complementati...

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Differential Expression of the Motin Family in the Peri-implantation Mouse Uterus and Their Hormonal Regulation

Matsumoto

Fukui

Yoshizawa

et al. 2012

Journal of Reproduction and Development

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“…Lipocalin-7 is a potent integrin ligand that has emerged as an important promoter of cell adhesion and spreading through interactions with ECM proteins and integrins (Tajiri et al, 2010). Brown et al (2010) demonstrated that lipocalin-7 is a positive regulator of angiogenesis that increases endothelial cell invasion, angiogenic cell …”

Section: Extracellular Integrin Ligandsmentioning

confidence: 99%

Proteins involved in focal adhesion signaling pathways are differentially regulated in experimental branch retinal vein occlusion

Cehofski¹,

Kruse

Kjærgaard

et al. 2015

Experimental Eye Research

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“…In particular, TINAGL1 is a novel component of the Reichert's membrane that interacts with laminin 1 [49]. In contrast, it associates with integrins a5 and b1 in the decidualized uterine endometrium [51].…”

Section: Extracellular Proteins and Tinagl1 In The Basement Membrane mentioning

confidence: 99%

Molecular and cellular events involved in the completion of blastocyst implantation

Matsumoto

Fukui

Yoshizawa

2015

Reprod Medicine & Biology

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Blastocyst implantation is an interactive process between the embryo and the uterus. The synchronization of embryonic development with uterine differentiation to a receptive state is essential for a successful pregnancy. The period of uterine receptivity for implantation is limited. Although implantation involves the interaction of numerous signaling molecules, our understanding of the hierarchical mechanisms that coordinate with the embryo-uterine dialogue is not yet sufficient to prevent infertility caused by implantation failure. This review highlights our knowledge on uterine receptivity and hormonal regulation of blastocyst implantation in mice. We also discuss the adhesion molecules, cross-linker proteins, extracellular proteins, and matricellular proteins involved in blastocyst implantation. Furthermore, our recent study reveals that selective proteolysis in an activated blastocyst is associated with the completion of blastocyst implantation after embryo transfer. A better understanding of uterine and blastocyst biology during the peri-implantation period would facilitate further development of reproductive technology.

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Tubulointerstitial Nephritis Antigen-Like 1 Is Expressed in the Uterus and Binds with Integrins in Decidualized Endometrium During Postimplantation in Mice1

Cited by 26 publications

References 27 publications

Differential Expression of the Motin Family in the Peri-implantation Mouse Uterus and Their Hormonal Regulation

Differential Expression of the Motin Family in the Peri-implantation Mouse Uterus and Their Hormonal Regulation

Proteins involved in focal adhesion signaling pathways are differentially regulated in experimental branch retinal vein occlusion

Molecular and cellular events involved in the completion of blastocyst implantation

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