Tuftelin 1 (TUFT1) Promotes the Proliferation and Migration of Renal Cell Carcinoma via PI3K/AKT Signaling Pathway

Lin, Hua; Zeng, Weifeng; Lei, Yuhang; Chen, Desheng; Nie, Zhen Gui

doi:10.3389/pore.2021.640936

Cited by 14 publications

(11 citation statements)

References 45 publications

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“…DEGs among different m7G subtypes were enriched in cancer-related pathways, such as the positive regulation of cell adhesion, the positive regulation of vasculature development, cell molecule adhesion, the PI3K–Akt signaling pathway, and the AKT–STAT signaling pathway. PI3K–Akt signaling pathway activation can promote the metastasis and progression of RCC ( Du et al, 2021 ; Lin et al, 2021 ; Zhu et al, 2020 ). We divided the patients into three gene types in accordance with the three m7G subtypes and found significant differences in OS and PFS among the subtypes.…”

Section: Discussionmentioning

confidence: 99%

m7G regulator-mediated molecular subtypes and tumor microenvironment in kidney renal clear cell carcinoma

Chen

Nie²,

Gao³

et al. 2022

Front. Pharmacol.

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Background: RNA methylation modification plays an important role in immune regulation. m7G RNA methylation is an emerging research hotspot in the RNA methylation field. However, its role in the tumor immune microenvironment of kidney renal clear cell carcinoma (KIRC) is still unclear.Methods: We analyzed the expression profiles of 29 m7G regulators in KIRC, integrated multiple datasets to identify a novel m7G regulator-mediated molecular subtype, and developed the m7G score. We evaluated the immune tumor microenvironments in m7G clusters and analyzed the correlation of the m7G score with immune cells and drug sensitivity. We tested the predictive power of the m7G score for prognosis of patients with KIRC and verified the predictive accuracy of the m7G score by using the GSE40912 and E-MTAB-1980 datasets. The genes used to develop the m7G score were verified by qRT-PCR. Finally, we experimentally analyzed the effects of WDR4 knockdown on KIRC proliferation, migration, invasion, and drug sensitivity.Results: We identified three m7G clusters. The expression of m7G regulators was higher in cluster C than in other clusters. m7G cluster C was related to immune activation, low tumor purity, good prognosis, and low m7G score. Cluster B was related to drug metabolism, high tumor purity, poor survival, and high m7G score. Cluster A was related to purine metabolism. The m7G score can well-predict the prognosis of patients with KIRC, and its prediction accuracy based on the m7G score nomogram was very high. Patients with high m7G scores were more sensitive to rapamycin, gefitinib, sunitinib, and vinblastine than other patients. Knocking down WDR4 can inhibit the proliferation, migration, and invasion of 786-0 and Caki-1 cells and increase sensitivity to sorafenib and sunitinib.Conclusion: We proposed a novel molecular subtype related to m7G modification and revealed the immune cell infiltration characteristics of different subtypes. The developed m7G score can well-predict the prognosis of patients with KIRC, and our research provides a basis for personalized treatment of patients with KIRC.

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Section: Discussionmentioning

confidence: 99%

m7G regulator-mediated molecular subtypes and tumor microenvironment in kidney renal clear cell carcinoma

Chen

Nie²,

Gao³

et al. 2022

Front. Pharmacol.

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“…TUFT1 is a well‐known carcinogen in multiple cancers, including hepatocellular carcinoma, 33 renal cell carcinoma (RCC), 34 and PC 14 . In this study, TUFT1 was highly expressed in PC.…”

Section: Discussionmentioning

confidence: 80%

“…32 In this study, miR-326-3p expression was reduced in PC cells, which was consistent with the findings of a previous study. 31 Combining bioinformatics analysis, luciferase assay, and RIP assay, we verified TUFT1 is a well-known carcinogen in multiple cancers, including hepatocellular carcinoma, 33 renal cell carcinoma (RCC), 34 and PC. 14 In this study, TUFT1 was highly expressed in PC.…”

Section: Discussionmentioning

confidence: 89%

LINC00960 regulates cell proliferation and glycolysis in pancreatic cancer through the miR‐326‐3p/TUFT1/AKT–mTOR axis

Zhou

Lei

Wang

et al. 2022

The Kaohsiung J of Med Scie

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Pancreatic cancer (PC) is a common malignant cancer characterized by high mortality and poor prognosis. LINC00690 was involved in the occurrence and progression of PC, but the underlying mechanisms require further investigation. The goal of this study was to figure out how LINC00960 mediates glycolysis in PC. LINC00960, miR-326-3p, and Tuftelin 1 (TUFT1) expression levels were detected in PC cell lines.LINC00960 and TUFT1 expression levels were increased in PC cells when compared with normal pancreatic cells, whereas miR-326-3p expression levels were decreased.The expression levels of LINC00690 affected glycolysis in PC, and inhibition of LINC00960 inhibited tumor growth in vivo. LINC00690 targeted and suppressed the expression of miR-326-3p. MiR-326-3p bound to TUFT1, and miR-326-3p inhibited AKT-mTOR pathway activation via TUFT1. In conclusion, the depletion of LINC00960 repressed cell proliferation and glycolysis in PC by mediating the miR-326-3p/TUFT1/AKT-mTOR axis. Thus, we present a novel mechanism underlying the progression of PC that suggests LINC00960 is a potential therapeutic target for this cancer.

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“…Our results revealed that kdelr2b , leap2 , krtcap2 , svep1 , s100a14 , colgalt1 , znf706 , mob1a , tmem200a , and bag2 were identified as hub genes of the PH trait-associated module. Although their functions in myogenesis have not been explored, many of these genes were reported to contribute to the cell proliferation process through different signaling pathways, such as the Akt-mTOR/GSK3β, PI3K/AKT, and ERK1/2 signaling pathways [ 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 ]. In addition, afmid , stip1 , xirp1 , tspan12 , mib2 , tekt4 , glg1 , stac3 , fam117a , and thrap3 were identified as hub genes of the DH trait-associated module.…”

Section: Discussionmentioning

confidence: 99%

Morphological and Molecular Responses of Lateolabrax maculatus Skeletal Muscle Cells to Different Temperatures

Zhang

Wen²,

Qi³

et al. 2022

IJMS

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Temperature strongly modulates muscle development and growth in ectothermic teleosts; however, the underlying mechanisms remain largely unknown. In this study, primary cultures of skeletal muscle cells of Lateolabrax maculatus were conducted and reared at different temperatures (21, 25, and 28 °C) in both the proliferation and differentiation stages. CCK-8, EdU, wound scratch and nuclear fusion index assays revealed that the proliferation, myogenic differentiation, and migration processes of skeletal muscle cells were significantly accelerated as the temperature raises. Based on the GO, GSEA, and WGCNA, higher temperature (28 °C) induced genes involved in HSF1 activation, DNA replication, and ECM organization processes at the proliferation stage, as well as HSF1 activation, calcium activity regulation, myogenic differentiation, and myoblast fusion, and sarcomere assembly processes at the differentiation stage. In contrast, lower temperature (21 °C) increased the expression levels of genes associated with DNA damage, DNA repair and apoptosis processes at the proliferation stage, and cytokine signaling and neutrophil degranulation processes at the differentiation stage. Additionally, we screened several hub genes regulating myogenesis processes. Our results could facilitate the understanding of the regulatory mechanism of temperature on fish skeletal muscle growth and further contribute to utilizing rational management strategies and promoting organism growth and development.

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Tuftelin 1 (TUFT1) Promotes the Proliferation and Migration of Renal Cell Carcinoma via PI3K/AKT Signaling Pathway

Cited by 14 publications

References 45 publications

m7G regulator-mediated molecular subtypes and tumor microenvironment in kidney renal clear cell carcinoma

m7G regulator-mediated molecular subtypes and tumor microenvironment in kidney renal clear cell carcinoma

LINC00960 regulates cell proliferation and glycolysis in pancreatic cancer through the miR‐326‐3p/TUFT1/AKT–mTOR axis

Morphological and Molecular Responses of Lateolabrax maculatus Skeletal Muscle Cells to Different Temperatures

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