Tumor angiogenesis and rectal carcinoma

Saclarides, Theodore J.; Speziale, Nicholas J.; Drab, E. A.; Szeluga, Debra J.; Rubin, David B.

doi:10.1007/bf02052599

Cited by 100 publications

(63 citation statements)

References 8 publications

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“…with deeper tissue invasion. This finding is comparable to previous reports which claim that the tumor spreading activity is fueled by angiogenesis (20,23,26) . CHOI et al (5) found a non-significant difference between high microvessel counts and deeper tumor invasion.…”

Section: Microvessel Count and Patient Survivalsupporting

confidence: 91%

Angiogenesis in advanced colorectal adenocarcinoma with special reference to tumoral invasion

Tarta

Teixeira

Tanaka

et al. 2002

Arq. Gastroenterol.

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-Background -Angiogenesis is a crucial step in tumor growth and progression. Its quantification by microvessel counting has a prognostic value in several types of malignancies and recently has been appraised in gastrointestinal tumors. Aim -To assess the prognostisc significance of microvessel quantification in colorectal carcinomas, studying its association with hematogenous metastases, survival and clinicopathological variables such as size, histologic differentiation and depth of tumoral invasion. Patients/Methods -Forty eight patients with colorectal adenocarcinoma were included in this study. Histologic sections of invasion tumoral margin (4 µm) were analyzed and endothellined microvessels were immunostained with monoclonal mouse Von Willebrand Factor (anti-FVIII). The microvessel count was performed from the identification of the area with increased microvessel density -hot spots -and results of the mean in five of these fields. Results -The cut-off microvessel count was 14 microvessels/0,785 mm 2 , which divided the sample into hypovascular and hypervascular groups. While 2/8 (25%) tumors with muscularis propria invasion were classified as hypervascular, 11/15 (73%) tumors with serosa or perivisceral fat were classified as hypervascular. However, a non-significant statistical association was found between the angiogenesis quantification, hematogenous metastases, survival and clinicopathological variables such as size and histologic differentiation of the tumor. Conclusions -The findings of significantly increase of microvessel count in conformity with tumoral invasion depth supports the hypothesis that tumor progression might be related to angiogenesis. Although angiogenesis is an important step in the tumoral growth and during the metastatization process, other factors can be implicated.

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Section: Microvessel Count and Patient Survivalsupporting

confidence: 91%

Angiogenesis in advanced colorectal adenocarcinoma with special reference to tumoral invasion

Tarta

Teixeira

Tanaka

et al. 2002

Arq. Gastroenterol.

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“…Microvessel density has been shown to be an independent parameter of the severity of tumour disease and a predictor of prognosis of breast cancer patients in most but not all studies addressing this issue (Weidner et al, 1991(Weidner et al, , 1992Bosari et al, 1992;Horak et al, 1992;Toi et al, 1993, Axelsson et al, 1995. A similar correlation has also been demonstrated with other human malignancies, including head and neck squamous cell carcinoma (Gasparini et al, 1993), lung adenocarcinoma (Yamazaki et al, 1994), rectal carcinoma (Saclarides et al, 1994), testicular germ cell tumours (Olivarez et al, 1994), prostate cancer , tumours of the oral cavity (Williams et al, 1994) and gastric carcinoma (Maeda et al, 1995 rie receptor in breast cancer P Salven et al higher when staining was performed using the clone 7E8 antibody (Table I). No specific staining was observed when the sections were incubated with antigen-blocked antibody instead of the primary antibodies, or with the peroxidaseconjugated secondary antibody only.…”

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confidence: 71%

Endothelial Tie growth factor receptor provides antigenic marker for assessment of breast cancer angiogenesis

Salvén

Joensuu²,

Heikkilä³

et al. 1996

Br J Cancer

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Summary Breast cancer prognosis has previously been linked to the degree of tumour vascularisation. In order to establish additional markers for tumour angiogenesis, we have used monoclonal antibodies against the endothelial Tie receptor tyrosine kinase to study the degree of vascularisation of breast carcinomas and the regulation of Tie expression in the vascular endothelial cells. Antibodies were used for Tie detection and the results were correlated with other prognostic markers. Of four monoclonal antibodies directed against different epitopes of the Tie extracellular domain, two reacted against Tie in unfixed histopathological sections of breast carcinomas. One of these antibodies (clone 7e8) was specific for the endothelial cells whereas the other (clone 10fl 1) also reacted with basement membranes and occasional carcinoma cells. When Tie expression was studied with the antibody clone 7e8, all 27 carcinomas, two in situ carcinomas, samples of histologically normal breast tissue (n = 16) or normal skin or lymph node tissue (n= 5) showed staining. Microvessel counts were higher in carcinomas (median 14; range 3 -27) than in fibrodenomas (median 10; range 5 -18) or histologically normal breast tissue (median 7; range 3 -15, P = 0.0006). A similar result was obtained using antibodies against the CD31 (PECAM) antigen. Microvessel counts in 7e8 staining were not significantly associated with primary tumour size, axillary nodal status, histological grade or staining for oestrogen receptor, progesterone receptor, Ki-67 proliferation marker or p53 oncoprotein.

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“…High MVD has been associated with a poorer outcome in some studies of CRC (Saclarides et al, 1994;Tanigawa et al, 1997). Our results concur with this, with high MVD primary cancers and liver metastases having a reduced disease-free survival.…”

Section: Discussionmentioning

confidence: 99%

“…In breast cancer (Uzzan et al, 2004), non-small cell lung carcinoma (Ushijima et al, 2001) and melanoma (Ribatti et al, 2003), a high MVD is associated with poor outcome. In CRC, a high MVD has been shown to predict disease recurrence and survival in some studies (Saclarides et al, 1994;Tanigawa et al, 1997), whereas other studies have shown an association between high MVD and improved survival (Prall et al, 2003) or have concluded that MVD failed to provide additional prognostic information (Cianchi et al, 2002).…”

mentioning

confidence: 99%

The influence of invasive growth pattern and microvessel density on prognosis in colorectal cancer and colorectal liver metastases

et al. 2007

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The nature of the invasive growth pattern and microvessel density (MVD) have been suggested to be predictors of prognosis in primary colorectal cancer (CRC) and colorectal liver metastases. The purpose of the present study was to determine whether these two histological features were interrelated and to assess their relative influence on disease recurrence and survival following surgical resection. Archival tissue was retrieved from 55 patients who had undergone surgical resection for primary CRC and matching liver metastases. The nature of the invasive margin was determined by haematoxylin and eosin (H&E) histochemistry. Microvessel density was visualised using immunohistochemical detection of CD31 antigen and quantified using image capture computer software. Clinical details and outcome data were retrieved by case note review and collated with invasive margin and MVD data in a statistical database. Primary CRCs with a pushing margin tended to form capsulated liver metastases (Po0.001) and had a significantly better disease-free survival than the infiltrative margin tumours (log rank P ¼ 0.01). Primary cancers with a high MVD tended to form high MVD liver metastases (P ¼ 0.007). Microvessel density was a significant predictor of disease recurrence in primary CRCs (P ¼ 0.006), but not liver metastases. These results suggest that primary CRCs and their liver metastases show common histological features. This may reflect common mechanisms underlying the tumour -host interaction.

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Tumor angiogenesis and rectal carcinoma

Abstract: Our results suggest that the growth of rectal cancer is dependent on the ingrowth of new blood vessels, and that increased vascularity promotes dissemination and adversely affects survival.

Cited by 100 publications

References 8 publications

Angiogenesis in advanced colorectal adenocarcinoma with special reference to tumoral invasion

Angiogenesis in advanced colorectal adenocarcinoma with special reference to tumoral invasion

Endothelial Tie growth factor receptor provides antigenic marker for assessment of breast cancer angiogenesis

The influence of invasive growth pattern and microvessel density on prognosis in colorectal cancer and colorectal liver metastases

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