Tumor angiogenesis as a predictor of recurrence in stage Ib squamous cell carcinoma of the cervix

Dinh, Tung Van; Hannigan, Edward V.; Smith, Edward; Hove, Maxwell G.M.; Chopra, Vimlarani; To, Tonia

doi:10.1016/0029-7844(96)00039-7

Cited by 44 publications

(30 citation statements)

References 11 publications

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“…Preliminary studies performed in our laboratory have shown that many tumour vessels are inadequately stained by using antibodies against factor VIII or CD31 antigens. Similar observations have been reported by others studying the vascularization of squamous cell carcinoma of the uterine cervix (Kainz et al, 1995;Wiggins et al, 1995;Dinh et al, 1996). The problem of varied staining was avoided in the present work by assessing the vascular density at a high magnification (x 410) without the use of specific endothelial stains.…”

Section: Discussionsupporting

confidence: 87%

Tumour hypoxia and vascular density as predictors of metastasis in squamous cell carcinoma of the uterine cervix

Sundfør

Lyng²,

Rofstad³

1998

Br J Cancer

169

106

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Summary Some clinical studies involving several histological types of cancer have suggested that high vascular density in the primary tumour promotes metastasis. Other studies have suggested that a high incidence of metastases is associated with low oxygen tension in the primary tumour. The purpose of the study reported here was to search for correlations between incidence of metastases and oxygen tension or vascular density in the same population of patients. Thirty-eight consecutive patients with squamous cell carcinoma of the uterine cervix were included in a prospective study. Pelvic, iliac and retroperitoneal lymph node metastases were detected by magnetic resonance imaging at the time of initial diagnosis. Oxygen tension was measured polarographically using the Eppendorf P02 Histograph 6650. Vascular density was determined by histological examination of tumour biopsies. The primary tumours of the patients with metastases (n = 19) were more poorly oxygenated than those of the patients without metastases (n = 19). Thus, the fractions of the P02 readings resulting in values below 5 mmHg and 10 mmHg were significantly higher for the former group of patients than for the latter (P = 0.03 and 0.02 respectively). In contrast, the vascular density of the primary tumour was not significantly different for the two groups of patients. The present study suggests that a high incidence of metastases in squamous cell carcinoma of the uterine cervix is associated with poor oxygenation of the primary tumour and not with a high vascular density.

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Section: Discussionsupporting

confidence: 87%

Tumour hypoxia and vascular density as predictors of metastasis in squamous cell carcinoma of the uterine cervix

Sundfør

Lyng²,

Rofstad³

1998

Br J Cancer

169

106

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“…In three studies early recurrence was significantly related with high vessel density (Schlenger et al, 1995;Bremer et al, 1996;Dinh et al, 1996). In our study we found a similar, but not significant, association.…”

Section: Discussionsupporting

confidence: 78%

“…Based on a review of the literature we chose JC70, an anti-CD31 antibody, as marker for the microvessels as this endothelial marker is the most sensitive and specific of all and it does not react with lymphatic endothelium (Parums et al, 1990;Horak et al, 1992;Kuzu et al, 1992). However, of the seven previously published studies about invasive cervical cancer and angiogenesis, six used factor VIII (Kainz et al, 1995;Rutgers et al, 1995;Schlenger et al, 1995;Wiggens et al, 1995;Dinh et al, 1996;Abulafia et al, 1996), one Ulex europeaus lectin I (Bremer et al, 1996) and none CD31 as endothelial marker.…”

Section: Discussionmentioning

confidence: 99%

“…In five out of the seven studies the vessel counting was performed manually (Kainz et al, 1995;Rutgers et al, 1995;Wiggens et al, 1995;Abulafia et al, 1996;Dinh et al, 1996). In the remaining two studies, the quantification of the tumour vascularity was performed by a computer.…”

Section: Discussionmentioning

confidence: 99%

“…In the published studies the amount of fields counted varies from unknown to 15, the magnification varies from 100 to 400 and the field size is only mentioned in three studies: 0.216 mm' (Abulafia et al, 1996), 0.739 mm2 (Dinh et al, 1996) and 0.74 mm2 (Kainz et al, 1995). According to Horak et al (1992) the magnification and/or field size influenced only the amount of vessels counted but not the results.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Quantification and prognostic relevance of angiogenic parameters in invasive cervical cancer

Tjalma

Marck²,

Weyler³

et al. 1998

Br J Cancer

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Summary Tumour stromal neovascularization was investigated in 114 invasive and 20 in situ carcinomas of the uterine cervix by staining representative sections with the specific endothelial marker anti CD31 (clone JC/70A, isotope IgG1). A digital image analyser was used to measure the immunoreactivity. The following parameters were determined in the 'hot spots': vessel counts, vessel perimeter and endothelial stained area (expressed per mm2). The results were correlated with clinical and histopathological data. There was no significant relationship between the histopathological findings (tumour histology, tumour differentiation, FIGO stage, presence of lymph node metastasis or lymphovascular space involvement) and the median vessel count. In a univariate analysis all angiogenesis parameters had prognostic value: a higher vascularity was associated with worse prognosis (P < 0.05). Multiple regression analysis showed that vascular permeation (P < 0.001) and the median vessel count (P = 0.005) were the most important prognostic indicators. In the future these criteria may be used for selection of patients for anti-angiogenesis therapy.

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Tumor angiogenesis as a prognostic factor in ovarian carcinoma

Schoell

Pieber

Reich

et al. 1997

Cancer

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Tumor angiogenesis as a predictor of recurrence in stage Ib squamous cell carcinoma of the cervix

Abstract: High microvessel count in tumors may be used to predict recurrence in stage Ib squamous cell carcinoma of the cervix.

Cited by 44 publications

References 11 publications

Tumour hypoxia and vascular density as predictors of metastasis in squamous cell carcinoma of the uterine cervix

Tumour hypoxia and vascular density as predictors of metastasis in squamous cell carcinoma of the uterine cervix

Quantification and prognostic relevance of angiogenic parameters in invasive cervical cancer

Tumor angiogenesis as a prognostic factor in ovarian carcinoma

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