Tumor Cell-Derived Exosomal circ-PRKCI Promotes Proliferation of Renal Cell Carcinoma via Regulating miR-545-3p/CCND1 Axis

Qian, Yiguan; Li, Yang; Xu, Luwei; Chen, Ke; Liu, Ning; Yang, Xiao; Lei, Qian; Li, Rongfei; Zhou, Changcheng; Xu, Zheng; Jia, Ru; Ge, Yu‐Zheng

doi:10.3390/cancers15010123

Cited by 13 publications

(9 citation statements)

References 32 publications

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“…Furthermore, CCND1 is a downstream protein of the p53, Wnt, and PI3K/AKT/mTOR pathways. [ 29 ] In this study, our results showed that the protein expression of CCND1 was downregulated by wogonin and miR‐27b‐5p mimic. YWHAZ could upregulated CCND1 protein, similar with the effect of miR‐27b‐5p inhibitor.…”

Section: Discussionmentioning

confidence: 60%

Wogonin inhibits hepatoma cell proliferation by targeting miR‐27b‐5p/YWHAZ axis

Ma,

Wang,

Wang

et al. 2023

J Biochem & Molecular Tox

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Wogonin (5,7‐dihydroxy‐8‐methoxyflavone), a natural flavonoid compound in herbal plants, can suppress growth in hepatocellular carcinoma (HCC). However, the microRNA (miRNA) expression profiles that are influenced by wogonin have not been thoroughly described. To explore the novel miRNAs and the biological mechanism underlying the effect of wogonin on HCC cells. The effect of wogonin on Huh7 cell growth was assessed both in vitro and in vivo. The expression profiles of miRNAs were obtained by small RNA sequencing. Luciferase reporter experiment and bioinformatics analysis were conducted to determine whether tyrosine 3‐monooxygenase/tryptophan 5‐monooxygenase activation protein zeta (YWHAZ) can bind to miR‐27b‐5p. Effects of the ectopic expression of YWHAZ and miR‐27b‐5p on Huh7 cells proliferation and apoptosis were evaluated. Furthermore, the cell cycle, apoptosis and multiple signaling pathway‐related molecules were detected by Western blot analysis. Wogonin substantially inhibited the growth of Huh7 cells both in vitro and in vivo. Seventy miRNAs exhibited greater than twofold changes in wogonin‐treated cells. Upregulation of miR‐27b‐5p inhibited Huh7 cell proliferation, and the anticancer effect of wogonin was reversed after miR‐27b‐5p knockdown. miR‐27b‐5p directly targeted YWHAZ in HCC cells. The proliferation‐inhibiting effect of miR‐27b‐5p was revoked by YWHAZ overexpression. Meanwhile, inhibition of HCC growth was achieved by downregulating YWHAZ. Wogonin exerted antitumor activity through multiple signaling molecules, such as focal adhesion kinase, protein kinase B, mammalian target of rapamycin and molecules related to apoptosis and cell cycle by upregulating miR‐27b‐5p and downregulating YWHAZ. Our findings suggest that miR‐27b‐5p/YWHAZ axis contributes to the inhibitory effect of wogonin in HCC by targeting related genes and multiple signaling pathways.

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Section: Discussionmentioning

confidence: 60%

Wogonin inhibits hepatoma cell proliferation by targeting miR‐27b‐5p/YWHAZ axis

Ma,

Wang,

Wang

et al. 2023

J Biochem & Molecular Tox

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“… 18 Exosomes contain many mediators that can transmit signals between cells, such as circRNAs, long non-coding RNAs (lncRNAs), miRNAs, mRNA, DNA, proteins, and lipids. 19 CircRNAs in exosomes are related to tumor proliferation, 19 , 20 , 21 , 22 , 23 prognosis, 24 and drug resistance, 25 and have the potential to be used as tumor markers. 26 …”

Section: Introductionmentioning

confidence: 99%

CircZCCHC2 decreases pirarubicin sensitivity and promotes triple-negative breast cancer development via the miR-1200/TPR axis

Zhang,

Wei,

Xie

et al. 2024

iScience

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“…TAM-secreted exosomal circ_0020256 promotes the proliferation and progression of cholangiocarcinoma by modulating the miR-432-5p/E2F3 axis [49]. In renal cell carcinoma (RCC), tumor-derived exosomal circ-PPKCI increases tumor cell proliferation via the miR-545-3p/CCND1 signaling pathway [50]. In HCC, adipocyte-derived exosomal circ-DB promotes tumor growth by suppressing miR-34a and activating the USP7/ Cyclin A2 signaling pathway [51].…”

Section: Exosomal Circrnas and Proliferation Of Cancer Cellsmentioning

confidence: 99%

Recent advances of exosomal circRNAs in cancer and their potential clinical applications

Yi,

Yue,

Liu

et al. 2023

J Transl Med

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Circular RNA (circRNA) is a type of non-coding RNA that forms a covalently closed, uninterrupted loop. The expression of circRNA differs among cell types and tissues, and various circRNAs are aberrantly expressed in a variety of diseases, including cancer. Aberrantly expressed circRNAs contribute to disease progression by acting as microRNA sponges, functional protein sponges, or novel templates for protein translation. Recent studies have shown that circRNAs are enriched in exosomes. Exosomes are spherical bilayer vesicles released by cells into extracellular spaces that mediate intercellular communication by delivering cargoes. These cargoes include metabolites, proteins, lipids, and RNA molecules. Exosome-mediated cell-cell or cell-microenvironment communications influence the progression of carcinogenesis by regulating cell proliferation, angiogenesis, metastasis as well as immune escape. In this review, we summarize the current knowledge about exosomal circRNAs in cancers and discuss their specific functions in tumorigenesis. Additionally, we discuss the potential value of exosomal circRNAs as diagnostic biomarkers and the potential applications of exosomal circRNA-based cancer therapy.

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Tumor Cell-Derived Exosomal circ-PRKCI Promotes Proliferation of Renal Cell Carcinoma via Regulating miR-545-3p/CCND1 Axis

Cited by 13 publications

References 32 publications

Wogonin inhibits hepatoma cell proliferation by targeting miR‐27b‐5p/YWHAZ axis

Wogonin inhibits hepatoma cell proliferation by targeting miR‐27b‐5p/YWHAZ axis

CircZCCHC2 decreases pirarubicin sensitivity and promotes triple-negative breast cancer development via the miR-1200/TPR axis

Recent advances of exosomal circRNAs in cancer and their potential clinical applications

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