Tumor cells from ultrasonic aspirations of glioblastomas migrate and form spheres with radial outgrowth

Abstract

Studies of primary cells from malignant brain tumors such as glioblastomas are limited by the small size of surgically resected specimens. However, glioblastomas are also frequently debulked via ultrasonic aspiration. In this study, we examined the functional competence and growth of their aspirated cells. Cells from minced tissue and aspirations were comparable in migration, formation of pseudopodia, development of cellular spheres with radial outgrowth, and neuroectodermal features. Cultures were maintained for more than six weeks without fibroblastic overgrowth. Our observations show that ultrasonically aspirated specimens contain cells useful for studies of tumor migration and growth of tumorspheres.

Identification of ATP citrate lyase as a positive regulator of glycolytic function in glioblastomas

“…In contrast, the levels of HSP90 in the primary cell pseudopodia were much lower, only 7% of the unmigrated cells. Strong positive staining of the pseudopodial lysate on this immunoblot for glial fibrillary acidic protein has been previously shown (16). Loading was approximately equalized by GAPDH.…”

“…Our method has been described. 16 Briefly, after sufficient tissue for a diagnosis was obtained, the solid region of a glioblastoma was debulked with an ultrasonic suction aspirator (Integra Neurosciences, Plainsboro, NJ) that generated a tumor cell suspension in saline and blood. The specimen was examined on smears to verify purity of the tumor sample.…”

Glioma Surgical Aspirate: A Viable Source of Tumor Tissue for Experimental Research

“…Previous studies have made use of tumor obtained using the CUSA: the tissue fragments were used for diagnostic procedures [3], cell culture [4,5,6] and for detection of oncogene amplifications [7]. This work extends these findings and validates the use of tumor tissue derived from the CUSA as an abundant and viable source of cells for analysing HGG heterogeneity using multi-parameter flow cytometry.…”

Glioma Spheroids Obtained via Ultrasonic Aspiration Are Viable and Express Stem Cell Markers

“…The number of spheroids analyzed was as follows: GFAP, n = 121 to 276; vWF, n = 154 to 338; Ki-67, n = 73 to 135; CD133, n = 169 to 413; Bmi-1, n = 141 to 278; nestin, n = 150 to 277; Sox2, n = 152 to 413; and Olig2, n = 102 to 426. tissue removed by ultrasonic aspiration of glioblastoma cells has been performed previously. 10 In this study, cell suspensions were obtained by ultrasonic aspiration from 3 patients. The results showed that these cells were able to migrate and form spheres similar to removed biopsy tissue.…”

“…However, different studies suggest that ultrasonic aspiration yields viable tumor cells and tumor tissue suitable for both culturing and diagnostics. [9][10][11][12][13][14][15][16] To the best of our knowledge, only 1 study has been performed with glioblastoma and showed that secondary, but not organotypic primary, glioma spheroids could be obtained from the aspirated cell suspensions. 10 In the present study, we hypothesized that tissue removed by ultrasonic aspiration could be used for establishing viable organotypic multicellular spheroids (OMSs), which are an increasingly important research tool.…”